PMC

Arsenic species and temporal profiles over three days from nine patients

A chromatogram obtained from HPLC–ICPMS analysis of an H₂O₂-treated chromatographic fraction containing MMA^III. Peak identities and HPLC–ICPMS conditions are the same as those for Fig. 

Chromatograms of saliva samples and the same samples spiked with authentic arsenic standards. Peak identities and high-performance liquid chromatography (HPLC)–ICPMS conditions were the same as those for Fig. . A chromatograms were obtained from a saliva sample (solid trace) and the same sample spiked with authentic MMA^III (dashed trace). B chromatograms were obtained from a saliva sample (solid trace) and the same sample spiked with MMMTA^V (dashed trace)

Temporal profiles showing the percentages of methylated arsenicals in the saliva of APL patient 4 (a) and patient 6 (b). The procedures for arsenic administration were the same as described for Fig. . Triangles MMA^V, circles DMA^V, diamonds sum of methylated arsenic

Chromatogram obtained from HPLC–electrospray ionization tandem mass spectrometry (ESI-MS/MS) analysis of synthesized MMMTA^V standard. Anion-exchange separation was performed using a Hamilton PRP-X100 column and a mobile phase comprising 50 % methanol and 5 mM ammonium formate (pH 6). ESI-MS/MS was performed in multiple reaction monitoring mode. Three characteristic ion transitions, 155/107, 155/121, and 155/137, of MMMTA^V were monitored simultaneously

Temporal profiles showing the concentrations of arsenic species in the saliva of APL patients. The patients received an intravenous infusion of a 10 mg dose of arsenic trioxide daily. The arrows indicate the time at which the infusion started. Collection of saliva samples from patient 4 (a) lasted 72 h with three arsenic (As^III) administrations of long infusion duration (about 18 h), and saliva samples from patient 6 (b) were collected for 52 h with two administrations of short infusion duration (below 4 h). Squares As^III, circles DMA^V, upright triangles MMA^V, inverted triangles As^V, diamonds sum of arsenic species

Typical chromatograms of arsenic standards (A) and saliva samples (B) from acute promyelocytic leukemia (APL) patients. Separation was achieved using a reversed-phase ODS-3 column, and a mobile phase comprising 5 mM tetrabutylammonium, 3 mM malonic acid, and 5 % methanol (pH 5.65). Arsenic was detected at m/z 75 using an Agilent 7500ce inductively coupled plasma mass spectrometry (ICPMS) instrument with an octopole reaction system. Peaks 1–8 correspond to As^III, monomethylarsonous acid (MMA^III), dimethylarsinic acid (DMA^V), monomethylarsonic acid (MMA^V), dimethylmonothioarsonic acid, dimethylarsinous acid (DMA^III), As^V, and monomethylmonothioarsonic acid (MMMTA^V), respectively