PMC

Core aliphatic and indolic GLS pathways in Arabidopsis thaliana. The enzyme that catalyzes each reaction is found to the side of the arrow. *Metabolizes short- and long-chain aliphatic GLS. ^Metabolizes long-chain aliphatic GLS. The aliphatic GLS pathway is bolded and is the focus of subsequent analyses. Adapted from figure 1 in and used with permission from Elsevier.

qRT-PCR comparison of HET with WT genotypes of the different insertion lines. UBQ10 was used as the reference gene to normalize the GLS gene (abbreviations on horizontal axis) of interest in each RNA extraction. All comparison between the expression of HET (dark gray bars) and WT (light gray bars) are significantly different except GSTF11 and GSTU20. The vertical hashes indicate standard error bars. Asterisks above each pair of bars show relative expression ratio of less than one, as judged by appropriate contrasts in ANOVA.