PMC

Increase in reoviral titers was observed in all 13 patients in whom baseline and post-treatment samples were available; titers reached their peak at 1 month following treatment initiation in the majority of patients.

Extensive necrosis of a resected metastatic melanoma lesion in a Reolysin^® treated patient. A sheet of viable melanoma cells is seen in the lower right with abrupt transition to the necrotic area at left. Ghost outlines of necrotic melanoma cells are seen in the upper cell.

Expression of reoviral proteins in post-treatment metastatic melanoma biopsies, as demonstrated with the Nuance system. (a) Hematoxylin and eosin (H&E) stain, (b) reovirus stain (fluorescent red), (c) tubulin stain (fluorescent green), (d) cells coexpressing both targets are fluorescent yellow, (e) higher magnification of an area of double positive cells indicative of productive reovirus infection (blue color, hematoxylin counterstain), (f) coexpression of reovirus (fluorescent red) with p38 (fluorescent green) in tumor cells results in fluorescent yellow.