PMC

Hierarchical clustering of cumulus cell samples (columns) and the 132 most significant probes (rows). Upregulated genes are marked in red, downregulated genes are marked in green. CC_GV (blue), cumulus cells from immature oocyte at germinal vesicle stage; CC_MII (pink), cumulus cells from mature oocyte.

Results were expressed as means ± SEM of relative expression to the reference gene RPL19. CC_B+, cumulus cells from mature oocyte yielding a blastocyst at day 5/6 of in vitro culture once fertilised; CC_B-, cumulus cells from mature oocyte which stopped developing at the embryo stage at day 5/6 of in vitro culture once fertilised; *, significant difference (p<0.05).

Hierarchical clustering of cumulus cell samples (columns) and the 354 most significant probes (rows). Upregulated genes are marked in red, downregulated genes are marked in green. CC_B- (blue), cumulus cells from mature oocyte which arrested at the embryo stage at day 5/6 of in vitro culture once fertilised; CC_B+ (red), cumulus cells from mature oocyte yielding a blastocyst at day 5/6 of in vitro culture once fertilised.

Patients were separated into two main groups: microarray and qPCR. The variability group was composed of patients who had one CC_B+ and at least one CC_B-. The pregnancy group was composed of CC_B+ transferred from patients included in the variability group. CC_B+, cumulus cells from a mature oocyte yielding a blastocyst at day 5/6 of in vitro culture once fertilised; CC_B-, cumulus cells from mature oocyte which stopped developing at the embryo stage at day 5/6 of in vitro culture once fertilised; CC_GV, cumulus cells from immature oocyte at germinal vesicle stage; P+, pregnancy; P-, no pregnancy.

Measurements of the 308 genes discriminating CC_B- and CC_B+ were extracted from different datasets: our study (A, B), GSE4260 (C), GSE18559 (D), GSE21005 (E) and GSE9526 (F). They were subjected to hierarchical clustering after log transformation and median centering of genes. The different types of sample are shown as coloured squares. The quality of the separation was measured by Fisher’s exact test on the main branch. CC_GV, cumulus cells from immature oocyte at germinal vesicle stage; CC_MII, cumulus cells from mature oocyte; CC_B+, cumulus cells from mature oocyte yielding a blastocyst at day 5/6 of in vitro culture once fertilised; CC_B-, cumulus cells from mature oocyte which stopped developing at the embryo stage at day 5/6 of in vitro culture once fertilised.