PMC

Pressor (A) and cardioaccelerator (B) responses to static contraction before, immediately after retrograde injection of glibenclamide (0.1 mg/kg) into the right femoral artery (Glib) and after subsequent retrograde injection of tempol (10 mg; Glib + Tempol) in rats whose left femoral arteries were ligated. Pressor (C) and cardioaccelerator (D) responses to tendon stretch, before, immediately after retrograde injection of glibenclamide (0.1 mg/kg), and after subsequent retrograde injection of tempol (10 mg) in rats whose femoral arteries were ligated. Values inside bars represent baseline means ± SE.

Pressor (A) and cardioaccelerator (B) responses to static contraction before (filled bars) and after (open bars) retrograde injection of tempol (10 mg) into the right femoral artery of rats whose left femoral arteries were ligated. Pressor (C) and cardioaccelerator (D) responses to tendon stretch before (filled bars) and after (open bars) intra-arterial injection of 10 mg of tempol in rats whose femoral arteries were ligated. Values inside bars represent baseline means ± SE. *P < 0.05, significant differences between means before and after tempol.

Pressor (A) and cardioaccelerator (B) responses to static contraction before, immediately after retrograde injection of iberiotoxin (IBTX; 20–40 μg/kg) into the right femoral artery, and after subsequent retrograde injection of tempol (10 mg; IBTX + Tempol) in rats whose left femoral arteries were ligated. Pressor (C) and cardioaccelerator (D) responses to tendon stretch before, immediately after retrograde injection of IBTX (20–40 μg/kg) into the right femoral artery and after subsequent retrograde injection of tempol (10 mg; IBTX + Tempol) in rats whose left femoral arteries were ligated. Values inside bars represent baseline means ± SE. *P < 0.05, significant difference between means for IBTX + Tempol and either before or after IBTX.

Pressor and cardioaccelerator responses to static contraction before (A) and after (B) retrograde injection of tempol (10 mg) into the right femoral artery of a rat whose left femoral artery was ligated 72 h before the start of the experiment. Note that the contraction-induced increases in arterial blood pressure (ABP) and heart rate (HR) were attenuated by tempol.

Individual Western blots (A) and summary data (B) showing that ligation of the left femoral artery 72 h before removing the left L4 and L5 dorsal root ganglia increased the pore-forming proteins (K_ir6.1 and K_ir6.2) of the K_ATP channel, but had no effect on the proteins comprising the BK_Ca channel (K_Ca1.1). *Significant difference between dorsal root ganglia taken from rats whose left femoral arteries were freely perfused (FP) and dorsal root ganglia taken from rats whose left femoral arteries were ligated (Lig).