Gpr179 Immunohistochemistry on Retinal Sections of Wild-Type Mice
GPR179 signal (green) in the OPL and ILM double labeled with other retinal markers (red) were detected in wild-type mice by confocal microscopy. The scale bar represents 20 μm. The following abbreviations are used: ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; GCL, ganglion cell layer; and ILM, inner limiting membrane. Gpr179 did not colocalize with the presynaptic marker Basoon (A), the ON-bipolar cell markers Goα (B) and PKCα (C), or with the ganglion cell labeled with calretinin (D). Some cells in the upper part of INL were surrounded by specific Gpr179 staining (arrow in A). The shape and the localization might indicate that these cells represent horizontal cells. Bipolar cell dendrites, stained with PKCα seem to surround Gpr179 (arrow in C). Calretinin antibody labeled specifically the ganglion cells, and their dendrites did not colocalize with Gpr179 (arrow in D). Instead, it seems that Gpr179 is highly concentrated in Müller cell endfeet (D); similar results have been previously shown for a potassium channel Kir4.1.