PMC

Distribution of DACH2 expression in fallopian tubes and ovarian cancer. Bar charts visualizing the staining distribution of DACH2 in (A) fallopian tubes and (B) ovarian cancer, and (C) in serous vs non-serous carcinoma. NS = nuclear score, e.g. a multiplier of fraction (0-3) and intensity (0-3) of staining.

Immunohistochemical images of DACH2 staining in fallopian tubes and ovarian cancer. Images (20× magnification) representing immunohistochemical expression of DACH2 in (A, B) fallopian tubes, and EOC ranging from (C) negative, (D) weak intensity in few cells, (E) weak intensity in majority of cells, (F) moderate to strong intensity in majority of cells, (G) strong intensity in majority of cells and (H) strong intensity in all tumour cells.

Kaplan-Meier estimates of ovarian cancer specific and overall survival in all patients according to DACH2 expression. Kaplan Meier analysis of ovarian cancer specific and overall survival in strata of low and high DACH2 expression in (A, C) all patients, and (B, D) serous carcinoma. The categories of staining were determined according to the nuclear score (NS), e.g. a multiplier of fraction and intensity, whereby low expression = NS < = 3 and high expression = NS > 3.

Expression of DACH2 in the cisplatin-sensitive A2780 ovarian cancer cell line compared to the cisplatin-resistant cell line A2780-Cp70 and validation of the specificity of the DACH2 antibody in A2780 ovarian cancer cells. Substantially higher DACH2 protein expression was seen in the cisplatin-resistant A2780-Cp70 cell line compared to its parental cisplatin-sensitive A2780 cell line by A) immunocytochemical staining and (B) immunoblotting, showing a major band at 62 kDa corresponding to the expected molecular weight of the DACH2 protein. (C) Relative mRNA expression was also higher in the A2780-Cp70 cells compared to A2780 cells as shown by qRT-PCR analysis. Data shown are mean ± SD of a representative experiment of two independent experiments performed in triplicate. (D) DACH2 protein expression was significantly decreased after transfection with siRNA against DACH2 in A2780-Cp70 cells as shown by immunocytochemistry 72 hrs post-transfection.