A, Dot-blot hybridization assay of mRNA from different tissues using the probes of 4Ig-B7-H3. Samples of PBMCs, PAM, heart, liver, spleen, lung, kidney, brain, lymph nodes, small intestine, tonsil were loaded from dot 1 to 11, respectively, with positive control (dot 12) and negative control (dot 13). The upper and lower panels were B7-H3 and β-actin, respectively. B, Analysis of the porcine B7-H3 isoforms. The normalized PCR products of PBMCs, PAM, heart, liver, spleen, lung, kidney, brain, lymph nodes, small intestine, tonsil were loaded from lane 2 to 12 with DNA ladder marker (lane 1). The upper and lower panels were the PCR products of B7-H3 and β-actin, respectively.