PVX infection in Arabidopsis ago mutants. A, Symptoms induced by PVX UK3 infection on wild type and ago1-27, ago2-1, ago3-2, ago4-2, ago5, ago6-3, ago7, ago8-1, ago9, and ago10 Arabidopsis mutants. Three leaves per plants were rub inoculated with sap made from PVX UK3-infected tobacco (PVX UK3). Photographs were taken 4 weeks after infection. B, Immunoblot analysis of PVX CP accumulation in inoculated leaves. Total protein extracts were prepared from inoculated leaves 5 d after inoculation and subjected to SDS-PAGE followed by anti-CP immunoblotting. Coomassie staining of the same extracts is shown to demonstrate equal loading. Each lane corresponds to a pool of the three inoculated leaves from a single plant. This result was obtained consistently in five experiments including at least three replicates for each genotype.