EBNALP does not effect EBNA2 association with enhancer or promoter DNA, and EBNA2 does not effect EBNALP association with enhancer or promoter DNA. (A) ChIP analysis from stable cell lines expressing vehicle control (BJ), EBNALP (LP), EBNA2 (E2), or EBNA2 and EBNALP (E2/LP) of enhancer and promoter regions of the EBNA2-regulated gene hes1, as indicated on top of each panel, using monoclonal EBNA2 antibody. As negative controls, IgG ChIP (Ctrl) on the E2 cell line and E2 ChIP on BJ cell line were performed. Columns represent average percentage DNA relative to input detected in duplicate qPCR reactions. Error bars represent SEM of at least three independent experiments. (B) As in A, but qPCRs were performed on the cd21 enhancer and promoter. (C and D) As in A and B, but using EBNALP antibodies. Control reactions using anti EBNALP antibodies were performed on the LP cell line.