(A) The percentage of CD45+ immune cells among total live cells present in the pancreas was quantified using flow cytometry. Data from 3 independent experiments are shown using control (N = 9), Ptf1a-Cre; KrasG12D (N = 8) and Ptf1a-Cre; KrasG12D; Stat3f/f (N = 5) mice.
(B) Relative expression of inflammatory mediators (IL-6, IL-11, IL-1β, Cox-2, ICAM1, Tnfα, and CCL2) in pancreata as measured by qPCR from control (N = 5), Ptf1a-Cre; KrasG12D (N = 8), and Ptf1a-Cre; KrasG12D; Stat3f/f (N = 8) mice one day after caerulein injection. Means ± SD.
(C) Relative expression of inflammatory mediators and Stat3 target genes (IL-6, IL-11, and Cox-2) in cultured primary acini isolated from wild-type and Ptf1a-Cre; Stat3f/f mice 20 hours after treatment with BSA or 10nM caerulein. Means ± SD (N = 3). * p < 0.05, ** p < 0.01. (Ptf1a-Cre; Kras, Ptf1a-Cre; Kras; Stat3, and Ptf1a-Cre; Stat3 represent Ptf1a-Cre; KrasG12D, Ptf1a-Cre; KrasG12D, and Ptf1a-Cre; KrasG12D; Stat3f/f mice, respectively).
See also .