PMC

Genomic organisation of the AAV and basic AAV vector design.

The genomic organisation of MoMLV and the basic retroviral vector design.

Features of adenovirus particle. A.) Structural features of Ad. B.) Genomic organisation of wild type Ad. Reproduced with modifications from original diagram provided kindly by Dr Simon Waddington.

Schematic representation of the human LDLR gene, mRNA and protein, A, B and C, respectively. UTR, untranslated region of the mRNA transcript. Reproduced with modifications from[].

Schematic representation of the improved retrovirus three plasmid co-transfection system. Viral genome is segregated into three expression plasmids; the vector genome, the Gag/Pol expressing plasmid, and the envelope expressing plasmid. For generation of viral particles, these plasmids are co-transfected into the HEK 293 producer cells, and virus is released into the supernatant.

Retrovirus virion, viral genome and RNA transcript. A.) Schematic diagram of virion structure. B.) The genome organisation of an oncoretrovirus provirus DNA; locations of all genes and the LTR are indicated. C.) Viral RNA transcripts. The full-length transcript serves as the RNA genome and as a messenger RNA for Gag and Gag/Pol polyproteins. The Env is translated from the spliced transcript.