(A) RT-PCR for Atp2c2, Mist1 or β-actin in RNA extracts from pancreas (Pan), testes (Tes), salivary glands (SG), seminal vesicle (SV) and liver (Liv). No RT (−) was added to the negative control. (B–I) IF for SPCA2 on salivary glands (B, C) and stomach (D–I) sections from WT (B, D, F, H) and Mist1−/− (C, E, G, I) mice. Arrows in B indicates basal localization of SPCA2 in salivary gland acini. Arrowheads in B and C indicate SPCA2 expression in duct cells of the salivary gland, which are not positive for MIST1. Arrowheads in D and E indicate SPCA2+ cells at the surface of epithelial crypts in both strains of mice while arrows indicate SPCA2 positive cells with the epithelial crypts only in WT stomach sections. Images F–I are higher magnifications of D and E to better reveal SPCA2+ cells. All panels identify nuclei through DAPI staining expect H and I. Scale bar = 50 μM.