PMC

The LOD profiling of the flowering time QTL on A02 in population DH38 under different experimental conditions. (This figure is available in colour at JXB online.)

The BrFLC2 primers used in this study. (a) Genomic structure of the A. thaliana FLC gene copied from . E, exon; the exon size is indicated in the white boxes. (b) FLC2^e4-7: FLC2-specific primers in exon 4 and exon 7 (). (c) FLC2^e1-4: FLC2-specific primers in exon 1 and exon 4 (). (d) FLC2^e3/4–e5/6: FLC2-specific primers in the junction of exon 3–4 and exon 5–6. (e) FLC2^e3-4: FLC2 intron 3 primers in exon 3 and exon 4.

Semi-quantitative RT-PCR using BrFLC2-specific primers (FLC2^e4-7 and FLC2^e1-4) in pooled samples (fifth leaf stage; first and second leaves) of early and late pools of DH lines vernalized for 0, 2, 5, 11, 21, and 31 d. (A) FLC2^e4-7; (B) FLC2^e1-4; (C) control gene GAPDH; (D) control gene GAPDH. E, early pool; L, late pool; M, 1 kb ladder.

Effect of vernalization (0, 2, 5, 11, 21, and 31 d) on flowering time in pools of selected DH38 lines (five lines in the early pool, five lines in the late pool) and parents yellow sarson (YS-143) and pakchoi (PC-175). Flowering time is expressed as ‘days to flowering’ from the transplanting date to flower opening. The average flowering times of five DH lines with three plants per pool are presented and the average flowering times of three parental plants are given. (This figure is available in colour at JXB online.)

BrFLC2 expression (fold change, 2ⁿ) in early and late pools of DH lines during development from seedling to seventh leaf stage plants for the different vernalization treatments (0, 2, 5, 11, 21, and 31 d). Fold change (2ⁿ) was obtained by the ΔΔC_T method () by using a calibrator sample from the fifth leaf stage (third and fourth leaves) of the late pool of DH lines without vernalization. s, seedling stage; c, cotyledons of plants in the third leaf stage; 3rd1st, first and second leaves together of a plant in the third leaf stage; 5th3rd, third and fourth leaves together of a plant in the fifth leaf stage; 5th1st, first and second leaves together of plant in the fifth leaf stage; 7th3rd, third and fourth leaves together of a plant in the seventh leaf stage; 7th1st, first and second leaves together of a plant in the seventh leaf stage. (This figure is available in colour at JXB online.)