PMC

The three plots show the normalized intensities in polar coordinate and the cluster definition from BeadStudio for NECS subjects. The clear separation suggests that the genotype calls are robust.

The four plots display the pattern of LD captured by the SNPs associated with exceptional longevity in ADARB1 (chromosome 21) using data from the NECS, SICS, AJCS and JCS. The intensity of red represents the strength of LD measured by D′.

Posterior densities of the ORs for the 3 SNPs in ADARB1 with rare alleles and moderate effects in the data aggregated from NECS, SICS and JCS. Significant associations would results in posterior densities not overlapping 1 and definite evidence of either an OR<1 or an OR>1, while all of the three densities have heavy tails and do not provide definite evidence against the null hypothesis of no association.

The four plots display the pattern of LD captured by the SNPs associated with exceptional longevity in ADARB2 (chromosome 10) in the NECS, SICS, AJCS and JCS data. The intensity of red cells represents the strength of LD measured by D′. The LD pattern in the NECS, SICS and AJCS subjects are very similar but differ substantially from the pattern of LD in the JCS subjects in which two SNPs become almost monomorphic (rs884949 and rs2387653). Highlighted in red are the SNPs that replicate the results in the AJCS and JCS subjects.

The two barplots show the age related trend of allele frequencies of SNPs rs17294019 (ADARB2, SNP # 98 in ) and rs3788157 (ADARB1, SNP # 135 in ) in the NECS (n = 1,023). The frequencies of the common allele for both SNPs were stratified in the age groups 90–99; 100–105, 106 and higher. Trends of allele frequencies for increasing age groups are consistent with a strong correlation between genotype and phenotype that results in substantial enrichment of protective alleles in older subjects.

a) Lifespan using mutant strains for adr-1;adr-2 in the context of dsRNA mediated gene inactivation of daf-2. Synchronized worms at the larval stage 4 (L4) were sterilized with FudR and allowed to feed on bacterial lawns that contained dsRNA for daf-2. Note: adr-1; adr-2 double mutant (red solid), adr-1; adr-2 double mutant with dsRNA for daf-2 (red hatched), N2 wild type (blue solid), N2 with dsRNA for daf-2 (blue hatched). Note decline in lifespan due to adr-1; adr-2 compared with N2 wildtype. Also note increases in lifespan of both N2 and adr-1; adr-2 in the presence of dsRNA for daf-2. The 50% survival time in the adr-1; adr-2 mutant animals was 10 days (95% limits 9 and 12 days) compared with 20 days (95% limits 18 and 20 days) for N2 wild-type control worms. RNAi to daf-2 increases lifespan to 34 days (95% limits 32 and 40 days), compared with 20 days for the wild type (N2 worms fed empty vector (RNAi)). daf-2 gene inactivation, in the background of the adr-1 and adr-2 null mutations also restored lifespan to 18 days (95% limits 16 and 20 days), compared with 10 days for the adr-1;adr-2 double mutant strain. b) Lifespan using mutant strains for adr-1;adr-2 (solid red), N2 wildtype (solid blue), rde-1 (grey hatched), adr-1; adr-2; rde-1 (grey solid) demonstrate declines in lifespan using mutant strains and full rescue of lifespan in an RNAi defective (rde-1) background. The adr-1; adr-2 mutant was again about half as long lived as wild-type (median survival time 9 days for adr-1;adr-2 strain (95% limits 9 and 11 days), and median survival time 21 days (95% limits 18 and 21 days), for N2 wild-type worms. The survival distribution of the triple mutant adr-1;adr-2; rde-1 is median lifespan 21 days (95% limits 18 and 21 days), which is significantly different from adr-1;adr-2, with a median lifespan of 9 days (95% limits 9 and 11 days). The lifespan of rde-1 was modestly reduced compared with the wild-type N2, as was reported previously²⁹. Inset boxes displays 50% survival (days) for each condition and demonstrates that daf-2 gene inactivation increases lifespan, in both wild type and in adr-1;adr-2 mutant strains (a) and that RNAi knockout (rde-1) restores lifespan.