PMC

Mixed skeletal muscle protein fractional synthesis rate (FSR) during basal postabsorptive conditions and during the hyperinsulinemic-hyperaminoacidemic-euglycemic clamp procedure in men and women. Data are means ± SE. ANOVA revealed a significant main effect for feeding (P = 0.003) but no effect of sex (P = 0.49) and no interaction (P = 0.93).

Average concentrations (arbitrary units) and representative blots (top: protein of interest; bottom: GADPH) of phosphorylated p70s6k^Thr389 (A), mTOR^Ser2448 (B), Akt^Ser473 (C), and Akt^Thr308 (D) during basal postabsorptive conditions and during the hyperinsulinemic-hyperaminoacidemic-euglycemic clamp procedure in men and women. Values are means ± SE.*ANOVA revealed a significant main effect for amino acid/insulin infusion (P ' 0.05) but no effect of sex and no interaction.

Average concentrations (arbitrary units) and representative blots (top: protein of interest; bottom: GADPH) of phosphorylated Erk 1/Erk 2 MAPK^Tyr202/204 (top) and eukaryotic elongation factor 2 (eEF2^Thr56; bottom) in muscle of men and women during basal postabsorptive conditions and during the hyperinsulinemic-hyperaminoacidemic-euglycemic clamp procedure. Values are means ± SE. ANOVA revealed a trend for a main effect of sex (Erk 1/Erk 2 MAPK, P = 0.051; eEF2, P = 0.11) but no effect of amino acid/insulin infusion and no interaction.