The cardioprotective effects of parstatin are dependent on NOS, sGC, and KATP channels. Inhibition of NOS (L-NMA, 100 µM), sGC (ODQ, 10 µM), or KATP channel (Glib, 1 µM). Isolated hearts were perfused with the inhibitors with or without parstatin (1 µM) before 30 min regional ischaemia and 180 min reperfusion. (A) Infarct size. (B) Recovery of LVDP. Data are mean ± SD, n = 6/group. *P < 0.05, treated vs. control. LVDP, left ventricular developed pressure.