PMC

Kaplan-Meier analyses of tumor doubling times.

Human (A) and mouse (B) VEGFR2 levels in LS174T tumors expressed as the percent change relative to untreated control xenografts.

Body weight changes after radiotherapy (mean±std dev). Values are corrected for tumor size and normalized to weights on the day of the first treatment.

Axial CT scan with tumor and bolus indicated (A). Tumor is within the 90% isodose line. Note the sharp dose fall-off medially and sparing of all non-tumor anatomy in the coronal reconstruction (B).

Expression of human and mouse VEGFR2 in LS174T xenografts 21 days after the first radiation dose. Untreated control mice (NT) were killed on day 15. A. Western immunoblot analyses of tumor lysates probed for human VEGFR2. β-Actin is the internal marker of the protein load. B. ELISA analyses of mouse VEGFR2 in the same lysates.

A. Tumor growth curves of LS174T xenografts implanted subcutaneously in athymic mice after radiotherapy. Values (mean±sem) are normalized to tumor volume on the day of the first treatment. B. Average tumor weights for all subjects in a given treatment group determined during necropsy. Solid line represents average tumor weights in untreated mice. Dotted lines are ±sem. C. Average tumor weights in mice with the initial tumor size of <0.4 g. D. Average tumor weights with the initial tumor size of >0.4 g (mean±sem).

Expression of VEGF and OPN in LS174T tumors collected at necropsy 21 days after treatment. A. Relationship between T_D and host (■) and tumor (●) VEGF. Mouse VEGF levels are directly proportional to T_D. B. Examples of normalized growth curves for individual LS174T xenografts either irradiated with 20 Gy dose at 1.2 Gy/min (thick lines) or left untreated (thin line). Several examples are shown to better illustrate strong relationship between mVEGF and tumor response. Numbers to the right of each line represent pg mVEGF/mg total protein in tumor lysates. C. Correlation between host OPN and host VEGF levels in tumor.