Sequence specificity for miR-373. (A) Mutations to four of the first or last base pairs in miR-373 resulted in miR-373–5MM and miR-373–3MM, respectively. The mutated bases are shown in blue. (B) PC-3 cells were transfected at 50 nM each indicated miRNA duplex. Expression of E-cadherin, CSDC2, and GAPDH were assessed by standard RT-PCR. GAPDH served as a loading control. (C) dsRNAs targeting divergent sites in either the E-cadherin or CSDC2 promoter specifically induce the expression of only the target gene. PC-3 cells were transfected with dsControl, miR-373, dsEcad-215, or dsCSDC2–670 for 72 h, as indicated. E-cadherin, CSDC2, and GAPDH mRNA expression levels were determined by standard RT-PCR.