PMC

Seroconversion to IgG anti-HEV in inoculated and control pigs. Panel A, pigs inoculated with a liver homogenate negative for HEV as negative controls (group 1); Panel B, pigs inoculated with a pool of two HEV-positive pig liver homogenate as positive controls (group 2); Panels C, D and E, each with a pool of two HEV-positive liver homogenates incubated at 56°C for 1 hr, stir-fried at 191°C for 5 min or boiled in water for 5 min (groups 3, 4 and 5, respectively). The ELISA cutoff OD value is 0.30.