PMC

Representative 1D ¹H NMR spectra of C) Control and following administration of cisplatin (D1) 24 hours (D2) 48 hours post-dosing (D3) 72 hours post-dosing.

PC scores plot showing the changes from control over a 72 hour period following administration of cisplatin (●) Control animals (Δ) 24 hours after dosing (■) 48 hours after dosing (+) 72 hours after dosing.

3D PCA scores plot of NMR data of day two urine indicating the effects of the PPARα ligand (WS) on cisplatin-induced ARF. (●) Control animals (■) Animals fed WY (Δ) Animals administered cisplatin following feeding with WY (+) Animals administered cisplatin.

. Effect of cisplatin on glucose levels in serum, urine, and kidney tissue. Mice were administered saline (control) or cisplatin (20 mg/kg body weight) by a single intraperitoneal injection. Glucose levels were measured at day1, day2, day3 after cisplatin injection in serum, urine, and kidney tissue homogenates as described in Methods. Bars correspond to means ± SE of at least 6 independent experiments under each condition. *P < 0.05, **P < 0.005 compared with control by unpaired student’s t-test.
. Effect of cisplatin and fibrate(WY) on glucose levels. Wild-type mice were fed with either a regular or WY-containing diet and then were given saline (control and WY groups) or cisplatin (cisplatin and cisplatin+WY groups). Glucose levels were measured at day 3 after cisplatin injection in serum, urine, and kidney tissue homogenates as described in Methods Results are expressed as the means ± SE. †P < 0.05compared to cisplatin, *P < 0.05, **P < 0.005 compared with control by unpaired Student’s t-test.

. Effect of cisplatin on NEFA levels in serum, urine and kidney tissue. Mice were administered saline (control) or cisplatin (20 mg/kg body weight) by a single intraperitoneal injection. NEFA levels were measured at day1, day2, day3 after cisplatin injection in serum, urine, and kidney tissue homogenates as described in Methods. Bars correspond to means ± SE of at least 6 independent experiments under each condition. *p < 0.05, compared with control by unpaired student’s t-test.
Effect of cisplatin and fibrate (WY) on NEFA levels. Wild-type mice were fed with either a regular or WY-containing diet and then were given saline (control and WY groups) or cisplatin (cisplatin and cisplatin + WY groups). NEFA levels were measured at day 3 after cisplatin injection in serum, urine, and kidney tissue homogenates as described in Methods Results are expressed as the means ± SE. †P < 0.05 compared to cisplatin, *P < 0.05, compared with control by unpaired Student’s t-test.

. Effect of cisplatin on TG levels in serum, urine and kidney tissue. Mice were administered saline (control) or cisplatin (20 mg/kg body weight) by a single intraperitoneal injection. TG levels were measured in serum, urine and kidney tissues at day1, day2, day3 after cisplatin injection as described in Methods. Bars correspond to means ± SE of at least 6 independent experiments under each condition. **p < 0.005 compared with control by unpaired student’s t-test.
B. Effect of cisplatin and fibrate (WY) on TG levels. Wild-type mice were fed with either a regular diet or WY-containing diet and then were given saline (control and WY groups) or cisplatin (cisplatin and cisplatin+WY groups). TG levels were measured in serum, and kidney tissue homogenates at day 3 after cisplatin injection as described in Methods. Results are expressed as the means ± SE. †P < 0.05 compared to cisplatin, *P < 0.05, **P < 0.005 compared with control by unpaired Student’s t-test.