PMC

Histogram showing the frequency of relative fold-change in probe sets with significant differences in transcript abundance between resistant (R) and sensitive (S) selection lines, pooled over sexes. The vertical dashed red lines demarcate two-fold changes in transcript abundance.

MET of lines containing P-element insertions in candidate genes. The white bar denotes the Canton S B co-isogenic control line; grey bars indicate lines with MET not significantly different from the control; blue bars indicate lines significantly sensitive to alcohol vapor to compare with the control (p < 0.05); and orange bars indicate lines significantly resistant than the control (p < 0.05). Error bars indicate standard errors.

Phenotypic response to selection for alcohol sensitivity. (a) MET for selection lines. Resistant lines are shown as orange squares, control lines as grey triangles, and sensitive lines as blue circles. Solid lines and shapes represent replicate 1; dashed lines and open shapes denote replicate 2. (b) Regressions of cumulative response on cumulative selection differential for divergence between resistant and sensitive selection lines. The blue line and squares represent replicate 1; the orange line and circles denote replicate 2.

Correlated phenotypic responses to selection. Lines with the same letter are not significantly different from one another at p < 0.05. Resistant lines are colored orange, control lines grey, and sensitive lines blue. Solid lines and bars represent replicate 1; dashed bars and lines denote replicate 2. (a) Locomotor reactivity; (b) climbing behavior; (c) aggression behavior; (d) starvation resistance; (e) olfactory avoidance behavior. Error bars indicate standard errors.