Activation of Sp1 transcription potential by E1AF. (A to D) PcDNA3.0 and/or E1AF and/or Sp1 expression vector was transiently cotransfected into SHG44 cells with a pSp1-Luc, pmSp1-Luc, CDK2-Luc, mCDK2-Luc, hSR-Luc, mhSR-Luc, AP2γ-Luc, or mAP2γ-Luc construct. The luciferase activity was determined as described in Materials and Methods. (E) PcDNA3.0 and/or E1AF and/or Sp1 expression vectors were transiently cotransfected into SHG44 cells with GalT V-Luc, M(Sp1), or M(EBS). The luciferase activity was determined as described in Materials and Methods. (F) PcDNA3.0 and/or E1AF(R397/400K) and/or Sp1 expression vector was transiently cotransfected into SHG44 cells with a pSp1-Luc, CDK2-Luc, hSR-Luc, AP2γ-Luc, or GalT V-Luc construct. The luciferase activity was determined as previously described.