Htz1 Prefers Promoters
(A and B) Htz1 strongly prefers promoters genome-wide.
(C and D) H2A weakly prefers nonpromoters. IGRs were assigned to one of three promoter-type classes (see text). Htz1 or H2A occupancy in tagged (A and C) or untagged control strains (B and D). Htz1 enrichment (log2 median ratio, x axis) versus the percent of IGRs in each promoter class (y axis). Strains for H2A ChIP: YBC2200 and YBC1894.
(E) Htz1 prefers promoters at individual genes. ChIP enrichment was determined by qPCR (note: RRP43 and RBK1 are divergent). Values are the average of three independent ChIPs with qPCR determination performed twice. Error bars: SD. The primer sets used for each amplicon are listed in ; format, (GeneID: promoter, ORF): YOR285W: D, F; YDL218W: I, K; YNL092W: O, Q; PRP12: U, V; YDC1: Y, AA; NUP159: AC, AE; MRK1: AF, AG; YNL116W: AH, AI; RIM11: AJ, AK; RRP43: AM, AL; RBK1: AM, AN. Values are normalized to an amplicon within iYMR325W, using primer set A.