PMC

Impact of genomic gains of 3p11-p12 on survival of patients with DLBCL. Kaplan-Meier survival estimates of patients with DLBCL with genomic gains of 3p11-p12 in comparison to their stratification into survival quartiles based on the gene-expression-based outcome predictor model alone (P = .029). Q indicates quartile.

Chromosomal imbalances influence the lymph node, proliferation, T-cell, and MHC class II gene-expression signatures. In each of the 4 panels, DLBCL cases are ordered according to their average expression of the respective signature genes (the case with the lowest expression appears on the left end of the spectrum). Cases with the chromosomal abnormalities shown on the right are marked with a yellow bar. Correlations with a P value less than .05 are shown. If more than one cytoband in one chromosomal arm showed a P value less than .05, the cytoband with the lowest P value is displayed.

Influence of chromosomal gains and amplifications on locus-specific gene-expression levels. Changes in gene-expression levels are depicted for each gene (averaged in each cohort) with regard to the locus-specific genetic status (wild-type vs gain vs amplification). Genes are ordered according to their chromosomal position shown on the right. Gene-locus information was obtained from the website for Genes On Sequence Map (Homo sapiens built 33). For genes with more than one microarray element on the Lymphochip, the average expression from different clones was calculated. The black bar on the left indicates the minimally gained region in all cases. The comparison of the expression levels was performed using the ANOVA test. Genes with significant differences (P < .01) are highlighted in red.

Summary of chromosomal imbalances detected in 224 cases of untreated de novo DLBCL classified by gene-expression profiling. Red bars on the left side of the ideogram indicate losses of chromosomal material; green bars on the right side indicate gains of chromosomal material; thick green bars indicate chromosomal gains exceeding the cut-off value of 1.5 in a large chromosomal region; solid dots indicate high-level DNA amplifications. Each bar represents a chromosomal region gained or lost in a single sample. (A) GCB-DLBCL (n = 87); (B) ABC-DLBCL (n = 77); (C) PMBCL (n = 19); and (D) bar diagram indicating the frequencies of chromosomal imbalances that distinguish between ABC-DLBCL, GCB-DLBCL, and PMBCL (for statistical details see “Patients, materials, and methods”). All differences were statistically significant at P < .05, with the exception of 12q12 gains (P = .059).