PMC

The morphology of the first two rosette leaves of wild-type (Col) and mutant plants.

The phenotype of zip-1, sgs3-11, rdr6-11, hst-1, and sqn-1, and plants homozygous for combinations of these mutations (13-day-old plants).

The effect of zip-1, sgs3-11, and rdr6-11 on posttranscriptional silencing of the L1 (35S::GUS) transgene. zip-1 does not interfere with the silencing of L1, but sgs3-11 and rdr6-11 prevent silencing in all fully expanded rosette leaves.

The phenotype of wild-type (Col) and mutant plants. (A) First leaf with abaxial trichomes. (B) Total number of leaves in the rosette. (C) Time to production of the first open flower. (D) Number of seeds per silique; 25-35 plants were measured for each genotype. Error bars indicate standard error of the mean.

The morphology of wild-type Columbia (Col), zip-1, sgs3-11, and rdr6-11. (A) Thirteen-day-old plants. The first few leaves of mutant plants are elongated and curl downward. (B) Flowers. Mutant plants have variable seed sets because stamens frequently fail to contact the stigma. (C) Scanning electron micrographs of carpels with one valve and seeds removed. Mutant carpels have a split septum and produce stigmatic tissue in the middle of the septum at the apical end of the carpels.

The effect of zip-1, sgs3-11, and rdr6-11 on the accumulation of mRNAs, miRNAs, and siRNAs. (A) mRNA of ARF3/ETTIN, ARF4, and SPL3 accumulates in zip, sgs3-11, and rdr6-11. Total RNA from 2-week-old rosettes of plants grown in short days was hybridized with probes to the indicated genes. Actin was used as a loading control. (B) SPL3 mRNA is elevated in zip-1 at 15 and 18 d after planting. Blot of total RNA from shoot apices of wild-type and zip-1 rosettes hybridized with a probe to SPL3. 18S ribosomal RNA was used as a loading control. (C) The accumulation of miRNAs in zip-1, sgs3-11, and rdr6-11. Blots of low-molecular-weight RNA from 2-week-old mutant and wild-type plants hybridized with probes complementary to the functional strand of the indicated miRNAs. (D) The effect of zip-1, sgs3-11, and rdr6-11 on the level of endogenous sRNAs. Blots of low-molecular-weight RNA from 2-week-old mutant and wild-type plants hybridized with probes to the indicated sRNAs. U6 was used as a loading control. The intensity of each signal was measured using NIH Image; the ratio of this signal relative to wild-type is indicated below each image.

SGS3 and RDR6 repress gene expression by promoting the production of trans-acting siRNAs. (A) Genes affected by sgs3-11 and rdr6-11, but not by zip-1. Total RNA from 2-week-old rosettes of plants grown in short days hybridized with probes to the indicated genes. Actin was used as a loading control. The intensity of each signal was measured using NIH Image; the ratio of this signal relative to wild-type is indicated below each image. (B) The genomic structure of At5g18040 and the location and sequence of the cognate site of sRNA255, sRNA752 and sRNA289, and sRNA850. UTRs are black, the coding region is blue, and the sRNA255 cognate sequence is red. Sequencing of nine 5′ RACE clones revealed that At5g18040 is cleaved in the middle of this cognate site. (C) 5′RACE products from Col, sgs3-11, and rdr6-11 2-week-old rosettes. This reaction gives one major product in Col (arrow-head) that is missing in sgs3-11 and rdr6-11. 5′RACE of SCL6-IV, which is cleaved by miR171, was used as a control. (D) sgs3-11, rdr6-11, and dcl1-7 block the production of sRNA255-like RNAs. Blot of low-molecular-weight RNA from 2-week-old plants hybridized with probes complementary to the indicated sRNAs. (E) sRNA255-like RNAs are found in three clusters on two different chromosomes. Arrows indicate the 5′-to-3′ orientation of the sRNA sequence. Red arrows are in direct orientation and blue arrows are in reverse orientation to the genomic DNA sequence.