PMC

Comparison of Rbf sequence and AraC family consensus sequence. The helix-turn-helix (HTH) motifs are indicated. Colons and periods indicate identical and similar residues, respectively.

Biofilm formation of NCTC8325-4, its isogenic rbf mutant, and a complemented strain on a glass side (A) and a polystyrene microtiter plate (B).

SDS-PAGE of whole-cell extracts from 8325-4(pLI50) (lane 1), rbf mutant CYL1097(pLI50) (lane 2), and CYL1097(pYL8565) (lane 3). Samples were run on a 10% gel. The arrow indicates the 190-kDa protein. Lane M, standards.

Effect of the rbf mutation on biofilm formation of a clinical isolate. The wild-type strain, its rbf mutant, and a complemented strain were tested for polystyrene binding in wells of a microtiter plate. The results represent the averages of three independent experiments. Error bars indicate the standard error of the mean. OD₄₉₀, optical density at 490 nm.

Biofilm formation in response to NaCl. The bacterial strains were tested for the effect of NaCl on biofilm formation as described in Materials and Methods. Results represent the averages of at least three independent experiments. Error bars indicate the standard error of the mean. OD₄₉₀, optical density at 490 nm.

Biofilm formation in response to glucose. The bacterial strains were tested for the effect of glucose on biofilm formation as described in Materials and Methods. Results represent the averages of at least three independent experiments. Error bars indicate the standard error of the mean. OD₄₉₀, optical density at 490 nm.