PMC

Methods of aeration that were assessed for maximum OTRs as shown in Table and Fig. . The solid lines inside the wells in the upper two panels represent the culture surface when the orbital shaker is at the outer right side of its rotating movement (as determined by photographic analysis). The dashed lines represent the culture surface when the orbital shaker is at the outer left side of its rotating movement.

Growth curves of P. putida CA-3 on a glucose mineral medium (750 μl) in deep-well microtiter plates incubated under various conditions: orbital shaking, 2.5-cm diameter, 300 rpm (⧫ and ◊); orbital shaking, 2.5-cm diameter, 375 rpm (■ and □); and orbital shaking, 5-cm diameter, 300 rpm (▴ and ▵). The dashed lines denote the slopes resulting from linear regression of the data points represented with solid symbols in the linear, oxygen-limited part of the growth curve. These slopes were used to calculate the OTRs given in Table .

System for the retrieval and growth of a bacterial strain collection in microtiter plate format. The spring-loaded replicator is used for the parallel sampling of up to 96 stock cultures frozen in glycerol; the surfaces of all stock cultures are contacted by the pins even though the surfaces are not exactly at the same level. The sampled cells are allowed to grow on an agar plate for several days. The colonies formed are then transferred to a square-well microtiter plate filled with liquid medium, covered as shown, and incubated on an orbital shaker.