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Volume 52(3);  March 2014

Articles from this journal are usually available in PMC after a 6-month delay (embargo). Some articles may be available sooner.


Editorial Board
J Clin Microbiol. Mar 2014; 52(3): A1. doi: 10.1128/JCM.masthead.52-3

Photo Quiz

Photo Quiz: A 47-Year-Old Female with General Fatigue, Fever, and Respiratory Symptoms
Dorothy Bilbo, Erik Munson
J Clin Microbiol. Mar 2014; 52(3): 711. doi: 10.1128/JCM.03462-12
Answer to March 2014 Photo Quiz
Dorothy Bilbo, Erik Munson
J Clin Microbiol. Mar 2014; 52(3): 1026. doi: 10.1128/JCM.03463-12


Detection of Intestinal Protozoa in the Clinical Laboratory
Ian H. McHardy, Max Wu, Robyn Shimizu-Cohen, Marc Roger Couturier, Romney M. Humphries
J Clin Microbiol. Mar 2014; 52(3): 712–720. doi: 10.1128/JCM.02877-13


Assessment of New Culture Method for Detection of Borrelia Species from Serum of Lyme Disease Patients
Barbara J. B. Johnson, Mark A. Pilgard, Theresa M. Russell
J Clin Microbiol. Mar 2014; 52(3): 721–724. doi: 10.1128/JCM.01674-13
Spectrum of Pneumococcal Serotype 11A Variants Results from Incomplete Loss of Capsule O-Acetylation
Juan J. Calix, Allison M. Brady, Victor Y. Du, Jamil S. Saad, Moon H. Nahm
J Clin Microbiol. Mar 2014; 52(3): 758–765. doi: 10.1128/JCM.02695-13
Automated versus Manual Sample Inoculations in Routine Clinical Microbiology: a Performance Evaluation of the Fully Automated InoqulA Instrument
P. Froment, H. Marchandin, P. Vande Perre, B. Lamy
J Clin Microbiol. Mar 2014; 52(3): 796–802. doi: 10.1128/JCM.02341-13
Microbial Biofilms on Needleless Connectors for Central Venous Catheters: Comparison of Standard and Silver-Coated Devices Collected from Patients in an Acute Care Hospital
Elizabeth Perez, Margaret Williams, Jesse T. Jacob, Mary Dent Reyes, Sheri Chernetsky Tejedor, James P. Steinberg, Lori Rowe, Satishkumar Ranganathan Ganakammal, Shankar Changayil, M. Ryan Weil, Rodney M. Donlan
J Clin Microbiol. Mar 2014; 52(3): 823–831. doi: 10.1128/JCM.02220-13
Controlled Clinical Comparison of BacT/Alert FA Plus and FN Plus Blood Culture Media with BacT/Alert FA and FN Blood Culture Media
T. J. Kirn, S. Mirrett, L. B. Reller, M. P. Weinstein
J Clin Microbiol. Mar 2014; 52(3): 839–843. doi: 10.1128/JCM.03063-13
Detection and Characterization of Mycoplasma pneumoniae during an Outbreak of Respiratory Illness at a University
Jessica L. Waller, Maureen H. Diaz, Brianna L. Petrone, Alvaro J. Benitez, Bernard J. Wolff, Laura Edison, Melissa Tobin-D'Angelo, Ashley Moore, Audrey Martyn, Hope Dishman, Cherie L. Drenzek, Kim Turner, Lauri A. Hicks, Jonas M. Winchell
J Clin Microbiol. Mar 2014; 52(3): 849–853. doi: 10.1128/JCM.02810-13
Identification and Clinical Significance of Helcococcus species, with Description of Helcococcus seattlensis sp. nov. from a Patient with Urosepsis
Siu-Kei Chow, Jill E. Clarridge, III
J Clin Microbiol. Mar 2014; 52(3): 854–858. doi: 10.1128/JCM.03076-13
Urine Is Not Sterile: Use of Enhanced Urine Culture Techniques To Detect Resident Bacterial Flora in the Adult Female Bladder
Evann E. Hilt, Kathleen McKinley, Meghan M. Pearce, Amy B. Rosenfeld, Michael J. Zilliox, Elizabeth R. Mueller, Linda Brubaker, Xiaowu Gai, Alan J. Wolfe, Paul C. Schreckenberger
J Clin Microbiol. Mar 2014; 52(3): 871–876. doi: 10.1128/JCM.02876-13
Fluoroquinolone Susceptibility Testing of Salmonella enterica: Detection of Acquired Resistance and Selection of Zone Diameter Breakpoints for Levofloxacin and Ofloxacin
Maria Sjölund-Karlsson, Rebecca L. Howie, John A. Crump, Jean M. Whichard
J Clin Microbiol. Mar 2014; 52(3): 877–884. doi: 10.1128/JCM.02679-13
Evaluation of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Rapid Detection of β-Lactam Resistance in Enterobacteriaceae Derived from Blood Cultures
Jette Sophia Jung, Christina Popp, Katrin Sparbier, Christoph Lange, Markus Kostrzewa, Soeren Schubert
J Clin Microbiol. Mar 2014; 52(3): 924–930. doi: 10.1128/JCM.02691-13
Identification, Genotypic Relation, and Clinical Features of Colistin-Resistant Isolates of Acinetobacter Genomic Species 13BJ/14TU from Bloodstreams of Patients in a University Hospital
Seung Yeob Lee, Jong Hee Shin, Kyung Hwa Park, Ju Hee Kim, Myung Geun Shin, Soon Pal Suh, Dong Wook Ryang, Soo Hyun Kim
J Clin Microbiol. Mar 2014; 52(3): 931–939. doi: 10.1128/JCM.02868-13
Comparison of Illumigene, Simplexa, and AmpliVue Clostridium difficile Molecular Assays for Diagnosis of C. difficile Infection
E. Deak, S. A. Miller, R. M. Humphries
J Clin Microbiol. Mar 2014; 52(3): 960–963. doi: 10.1128/JCM.02354-13
The Seventh Pandemic Vibrio cholerae O1 El Tor Isolate in China Has Undergone Genetic Shifts
Ping Zhang, Fengjuan Li, Weili Liang, Jie Li, Biao Kan, Duochun Wang
J Clin Microbiol. Mar 2014; 52(3): 964–967. doi: 10.1128/JCM.03121-13
Intermediate Susceptibility to Ciprofloxacin among Salmonella enterica Serovar Typhi Isolates in Lima, Peru
Coralith García, Veerle Lejon, Gertrudis Horna, Lizeth Astocondor, Raymond Vanhoof, Sophie Bertrand, Jan Jacobs
J Clin Microbiol. Mar 2014; 52(3): 968–970. doi: 10.1128/JCM.02663-13
Evaluation of Two Commercial Real-Time PCR Assays for Detection of Mycoplasma genitalium in Urogenital Specimens
Chloé Le Roy, Sabine Pereyre, Cécile Bébéar
J Clin Microbiol. Mar 2014; 52(3): 971–973. doi: 10.1128/JCM.02567-13
Novel Real-Time PCR Assays Using TaqMan Minor Groove Binder Probes for Identification of Fecal Carriage of Streptococcus bovis/Streptococcus equinus Complex from Rectal Swab Specimens
Paulo Guilherme Markus Lopes, Vlademir Vicente Cantarelli, Grasiela Agnes, Ane Micheli Costabeber, Pedro Alves d'Azevedo
J Clin Microbiol. Mar 2014; 52(3): 974–976. doi: 10.1128/JCM.03253-13
Assessment of Microbiological Diagnostic Procedures for Respiratory Specimens from Cystic Fibrosis Patients in German Laboratories by Use of a Questionnaire
Laura Häfner, Georg Peters, Barbara C. Kahl
J Clin Microbiol. Mar 2014; 52(3): 977–979. doi: 10.1128/JCM.02866-13
Effect of the Initial Specimen Diversion Technique on Blood Culture Contamination Rates
Khalifa Binkhamis, Kevin Forward
J Clin Microbiol. Mar 2014; 52(3): 980–981. doi: 10.1128/JCM.02773-13
Rapid Ertapenem Susceptibility Testing and Klebsiella pneumoniae Carbapenemase Phenotype Detection in Klebsiella pneumoniae Isolates by Use of Automated Microscopy of Immobilized Live Bacterial Cells
Carey-Ann D. Burnham, Rachel A. Frobel, Monica L. Herrera, Brian L. Wickes
J Clin Microbiol. Mar 2014; 52(3): 982–986. doi: 10.1128/JCM.03255-13
Rapid Resistome Fingerprinting and Clonal Lineage Profiling of Carbapenem-Resistant Klebsiella pneumoniae Isolates by Targeted Next-Generation Sequencing
Fabio Arena, P. Alexander Rolfe, Graeme Doran, Viola Conte, Sarah Gruszka, Thomas Clarke, Yemi Adesokan, Tommaso Giani, Gian Maria Rossolini
J Clin Microbiol. Mar 2014; 52(3): 987–990. doi: 10.1128/JCM.03247-13
Evaluation of the New Brilliance GBS Chromogenic Medium for Screening of Streptococcus agalactiae Vaginal Colonization in Pregnant Women
Paul O. Verhoeven, Pauline Noyel, Julie Bonneau, Anne Carricajo, Nathalie Fonsale, Alain Ros, Bruno Pozzetto, Florence Grattard
J Clin Microbiol. Mar 2014; 52(3): 991–993. doi: 10.1128/JCM.02926-13

Chlamydiology and Rickettsiology

Sabine Dittrich, Josée Castonguay-Vanier, Catrin E. Moore, Narongchai Thongyoo, Paul N. Newton, Daniel H. Paris
J Clin Microbiol. Mar 2014; 52(3): 832–838. doi: 10.1128/JCM.02786-13

Mycobacteriology and Aerobic Actinomycetes

Predicting Extensively Drug-Resistant Mycobacterium tuberculosis Phenotypes with Genetic Mutations
Timothy C. Rodwell, Faramarz Valafar, James Douglas, Lishi Qian, Richard S. Garfein, Ashu Chawla, Jessica Torres, Victoria Zadorozhny, Min Soo Kim, Matt Hoshide, Donald Catanzaro, Lynn Jackson, Grace Lin, Edward Desmond, Camilla Rodrigues, Kathy Eisenach, Thomas C. Victor, Nazir Ismail, Valeru Crudu, Maria Tarcela Gler, Antonino Catanzaro
J Clin Microbiol. Mar 2014; 52(3): 781–789. doi: 10.1128/JCM.02701-13
Evaluation of the AID TB Resistance Line Probe Assay for Rapid Detection of Genetic Alterations Associated with Drug Resistance in Mycobacterium tuberculosis Strains
C. Ritter, K. Lucke, F. A. Sirgel, R. W. Warren, P. D. van Helden, E. C. Böttger, G. V. Bloemberg
J Clin Microbiol. Mar 2014; 52(3): 940–946. doi: 10.1128/JCM.02597-13


Development of a Luminex-Based Multiplex Assay for Detection of Mutations Conferring Resistance to Echinocandins in Candida glabrata
Cau D. Pham, Carol B. Bolden, Randall J. Kuykendall, Shawn R. Lockhart
J Clin Microbiol. Mar 2014; 52(3): 790–795. doi: 10.1128/JCM.03378-13
Impact of New Antifungal Breakpoints on Antifungal Resistance in Candida Species
Annette W. Fothergill, Deanna A. Sutton, Dora I. McCarthy, Nathan P. Wiederhold
J Clin Microbiol. Mar 2014; 52(3): 994–997. doi: 10.1128/JCM.03044-13


Development of Two Antibody Detection Enzyme-Linked Immunosorbent Assays for Serodiagnosis of Human Chronic Fascioliasis
Kimberly Cabán-Hernández, José F. Gaudier, Caleb Ruiz-Jiménez, Ana M. Espino
J Clin Microbiol. Mar 2014; 52(3): 766–772. doi: 10.1128/JCM.02875-13
Detection of Trichomonas vaginalis DNA by Use of Self-Obtained Vaginal Swabs with the BD ProbeTec Qx Assay on the BD Viper System
Barbara Van Der Pol, James A. Williams, Stephanie N. Taylor, Catherine L. Cammarata, Charles A. Rivers, Barbara A. Body, Melinda Nye, Deanna Fuller, Jane R. Schwebke, Mathilda Barnes, Charlotte A. Gaydos
J Clin Microbiol. Mar 2014; 52(3): 885–889. doi: 10.1128/JCM.02966-13


Incorporation of a Proteotyping Approach Using Mass Spectrometry for Surveillance of Influenza Virus in Cell-Cultured Strains
Neil D. Fernandes, Kevin M. Downard
J Clin Microbiol. Mar 2014; 52(3): 725–735. doi: 10.1128/JCM.02315-13
Impact of Proficiency Testing Program for Laboratories Conducting Early Diagnosis of HIV-1 Infection in Infants in Low- to Middle-Income Countries
Albert Garcia, Shambavi Subbarao, Guoqing Zhang, Linda Parsons, John Nkengasong, Chin-Yih Ou, Dennis Ellenberger
J Clin Microbiol. Mar 2014; 52(3): 773–780. doi: 10.1128/JCM.03097-13
PhyloFlu, a DNA Microarray for Determining the Phylogenetic Origin of Influenza A Virus Gene Segments and the Genomic Fingerprint of Viral Strains
Luis F. Paulin, María de los D. Soto-Del Río, Iván Sánchez, Jesús Hernández, Rosa M. Gutiérrez-Ríos, Irma López-Martínez, Rosa M. Wong-Chew, Aurora Parissi-Crivelli, P. Isa, Susana López, Carlos F. Arias
J Clin Microbiol. Mar 2014; 52(3): 803–813. doi: 10.1128/JCM.03134-13
Diagnostic Potential and Antigenic Properties of Recombinant Tick-Borne Encephalitis Virus Subviral Particles Expressed in Mammalian Cells from Semliki Forest Virus Replicons
Lev Levanov, Suvi Kuivanen, Andrey Matveev, Sathyamangalam Swaminathan, Anu Jääskeläinen-Hakala, Olli Vapalahti
J Clin Microbiol. Mar 2014; 52(3): 814–822. doi: 10.1128/JCM.02488-13
Limited HIV-1 Superinfection in Seroconverters from the CAPRISA 004 Microbicide Trial
Andrew D. Redd, Caroline E. Mullis, Sarah K. Wendel, Daniel Sheward, Craig Martens, Daniel Bruno, Lise Werner, Nigel J. Garrett, Quarraisha Abdool Karim, Carolyn Williamson, Stephen F. Porcella, Thomas C. Quinn, Salim S. Abdool Karim
J Clin Microbiol. Mar 2014; 52(3): 844–848. doi: 10.1128/JCM.03143-13
Clinical Validation of the HPV-Risk Assay, a Novel Real-Time PCR Assay for Detection of High-Risk Human Papillomavirus DNA by Targeting the E7 Region
A. T. Hesselink, J. Berkhof, M. L. van der Salm, A. P. van Splunter, T. H. Geelen, F. J. van Kemenade, M. G. B. Bleeker, D. A. M. Heideman
J Clin Microbiol. Mar 2014; 52(3): 890–896. doi: 10.1128/JCM.03195-13
Ferdaus Hassan, Ashley Nguyen, Ashley Formanek, James J. Bell, Rangaraj Selvarangan
J Clin Microbiol. Mar 2014; 52(3): 906–910. doi: 10.1128/JCM.02484-13
Identification of Past and Recent Parvovirus B19 Infection in Immunocompetent Individuals by Quantitative PCR and Enzyme Immunoassays: a Dual-Laboratory Study
Peter A. C. Maple, Lea Hedman, Pravesh Dhanilall, Kalle Kantola, Visa Nurmi, Maria Söderlund-Venermo, Kevin E. Brown, Klaus Hedman
J Clin Microbiol. Mar 2014; 52(3): 947–956. doi: 10.1128/JCM.02613-13
Characterization of a Novel Melt Curve by Use of the Roche LightCycler HSV 1/2 Analyte-Specific Reagent Real-Time PCR Assay: Frequencies of This Novel (Low) Melt Curve and Commonly Encountered (Intermediate) Melt Curves
Bobby L. Boyanton, Jr., Amro Almradi, Mark J. Espy, Anne E. Prada, John P. Gibson, Bobbi S. Pritt
J Clin Microbiol. Mar 2014; 52(3): 957–959. doi: 10.1128/JCM.03062-13

Clinical Veterinary Microbiology

Pork Meat as a Potential Source of Salmonella enterica subsp. arizonae Infection in Humans
Grammato Evangelopoulou, Spyridon Kritas, Alexander Govaris, Angeliki R. Burriel
J Clin Microbiol. Mar 2014; 52(3): 741–744. doi: 10.1128/JCM.02933-13
High-Throughput Direct Fecal PCR Assay for Detection of Mycobacterium avium subsp. paratuberculosis in Sheep and Cattle
Karren M. Plain, Ian B. Marsh, Anna M. Waldron, Francesca Galea, Ann-Michele Whittington, Vanessa F. Saunders, Douglas J. Begg, Kumudika de Silva, Auriol C. Purdie, Richard J. Whittington
J Clin Microbiol. Mar 2014; 52(3): 745–757. doi: 10.1128/JCM.03233-13


Comparison of the Cerebrospinal Fluid (CSF) Toluidine Red Unheated Serum Test and the CSF Rapid Plasma Reagin Test with the CSF Venereal Disease Research Laboratory Test for Diagnosis of Neurosyphilis among HIV-Negative Syphilis Patients in China
Lin Zhu, Xin Gu, Rui-Rui Peng, Cuini Wang, Zixiao Gao, Pingyu Zhou, Ying Gao, Mei Shi, Zhifang Guan, Arlene C. Seña
J Clin Microbiol. Mar 2014; 52(3): 736–740. doi: 10.1128/JCM.02522-13
Panton-Valentine Leukocidin-Positive Staphylococcus aureus in Ireland from 2002 to 2011: 21 Clones, Frequent Importation of Clones, Temporal Shifts of Predominant Methicillin-Resistant S. aureus Clones, and Increasing Multiresistance
Anna C. Shore, Sarah C. Tecklenborg, Gráinne I. Brennan, Ralf Ehricht, Stefan Monecke, David C. Coleman
J Clin Microbiol. Mar 2014; 52(3): 859–870. doi: 10.1128/JCM.02799-13
Molecular Epidemiology of Enterococcal Bacteremia in Australia
Geoffrey W. Coombs, Julie C. Pearson, Denise A. Daley, Tam Le, Owen J. Robinson, Thomas Gottlieb, Benjamin P. Howden, Paul D. R. Johnson, Catherine M. Bennett, Timothy P. Stinear, John D. Turnidge
J Clin Microbiol. Mar 2014; 52(3): 897–905. doi: 10.1128/JCM.03286-13
High Frequency of Acinetobacter soli among Acinetobacter Isolates Causing Bacteremia at a Tertiary Hospital in Japan
Shiro Endo, Hisakazu Yano, Hajime Kanamori, Shinya Inomata, Tetsuji Aoyagi, Masumitsu Hatta, Yoshiaki Gu, Koichi Tokuda, Miho Kitagawa, Mitsuo Kaku
J Clin Microbiol. Mar 2014; 52(3): 911–915. doi: 10.1128/JCM.03009-13
Real-Time PCR Threshold Cycle Cutoffs Help To Identify Agents Causing Acute Childhood Diarrhea in Zanzibar
Kristina Elfving, Maria Andersson, Mwinyi I. Msellem, Christina Welinder-Olsson, Max Petzold, Anders Björkman, Birger Trollfors, Andreas Mårtensson, Magnus Lindh
J Clin Microbiol. Mar 2014; 52(3): 916–923. doi: 10.1128/JCM.02697-13

Case Reports

Report of the 13th Vancomycin-Resistant Staphylococcus aureus Isolate from the United States
Brandi M. Limbago, Alexander J. Kallen, Wenming Zhu, Paula Eggers, Linda K. McDougal, Valerie S. Albrecht
J Clin Microbiol. Mar 2014; 52(3): 998–1002. doi: 10.1128/JCM.02187-13
Hemolytic Uremic Syndrome following Infection with O111 Shiga Toxin-Producing Escherichia coli Revealed through Molecular Diagnostics
Darwin J. Operario, Shannon Moonah, Eric Houpt
J Clin Microbiol. Mar 2014; 52(3): 1003–1005. doi: 10.1128/JCM.02855-13
Fatal Subacute Myocarditis Associated with Human Bocavirus 2 in a 13-Month-Old Child
Amélie Brebion, Philippe Vanlieferinghen, Pierre Déchelotte, Morgane Boutry, Hélène Peigue-Lafeuille, Cécile Henquell
J Clin Microbiol. Mar 2014; 52(3): 1006–1008. doi: 10.1128/JCM.03013-13
Bacteremia Caused by Comamonas kerstersii in a Patient with Diverticulosis
Onya Opota, Barbara Ney, Giorgio Zanetti, Katia Jaton, Gilbert Greub, Guy Prod'hom
J Clin Microbiol. Mar 2014; 52(3): 1009–1012. doi: 10.1128/JCM.02942-13
Three Cases of Postoperative Septic Arthritis Caused by Mycobacterium conceptionense in the Shoulder Joints of Immunocompetent Patients
Keun Hwa Lee, Sang Taek Heo, Sung-Wook Choi, Da Hee Park, Young Ree Kim, Seung Jin Yoo
J Clin Microbiol. Mar 2014; 52(3): 1013–1015. doi: 10.1128/JCM.02652-13
Isavuconazole Treatment of a Patient with Disseminated Mucormycosis
Driele Peixoto, Lisa S. Gagne, Sarah P. Hammond, Erin T. Gilmore, Amy C. Joyce, Robert J. Soiffer, Francisco M. Marty
J Clin Microbiol. Mar 2014; 52(3): 1016–1019. doi: 10.1128/JCM.03176-13

Letters to the Editor

Haitian Variant tcpA in Vibrio cholerae O1 El Tor Strains in Kolkata, India
Priyanka Ghosh, Arindam Naha, Surajit Basak, Santanu Ghosh, T. Ramamurthy, Hemanta Koley, Ranjan K. Nandy, Sumio Shinoda, Haruo Watanabe, Asish K. Mukhopadhyay
J Clin Microbiol. Mar 2014; 52(3): 1020–1021. doi: 10.1128/JCM.03042-13
Absence of Pleocytosis Alone Is Insufficient To Exclude Encephalitis Caused by Herpes Simplex Virus in Children
Fiona Muttalib, Jesse Papenburg
J Clin Microbiol. Mar 2014; 52(3): 1022. doi: 10.1128/JCM.03262-13
Reply to “Absence of Pleocytosis Alone Is Insufficient To Exclude Encephalitis Caused by Herpes Simplex Virus in Children”
Paula López Roa, Roberto Alonso, Viviana de Egea, Patricia Muñoz, Emilio Bouza
J Clin Microbiol. Mar 2014; 52(3): 1023. doi: 10.1128/JCM.03321-13

Author Correction

Davide Abate, Alda Saldan, Carlo Mengoli, Marta Fiscon, Cristina Silvestre, Loredana Fallico, Marta Peracchi, Lucrezia Furian, Riccardo Cusinato, Luciana Bonfante, Barbara Rossi, Francesco Marchini, Dino Sgarabotto, Paolo Rigotti, Giorgio Palù
J Clin Microbiol. Mar 2014; 52(3): 1025. doi: 10.1128/JCM.00110-14


Answer to January 2014 Photo Quiz
Ben A. Zaniello, Geoffrey S. Gottlieb
J Clin Microbiol. Mar 2014; 52(3): 1024. doi: 10.1128/JCM.03576-13

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)