Volume 65(Pt 10); October 1, 2009
Editorial
Structural Communications
Structures of two truncated phage-tail hyaluronate lyases from Streptococcus pyogenes serotype M1
The crystal structures of truncated forms of the Streptococcus pyogenes phage-encoded hyaluronate lyases HylP2 and HylP3 have been determined by molecular replacement to 1.6 and 1.9 Å resolution, respectively.
PMCID: PMC2765878
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Use of thallium to identify monovalent cation binding sites in GroEL
Monovalent cation-binding sites in GroEL were identified utilizing the anomalous scattering signal from Tl+.
PMCID: PMC2765879
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Structure of Mycobacterium tuberculosis Rv2714, a representative of a duplicated gene family in Actinobacteria
The crystal structure of Rv2714, a protein of unknown function from M. tuberculosis, has been determined at 2.6 Å resolution using single-wavelength anomalous diffraction methods.
PMCID: PMC2765880
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New structural insights and molecular-modelling studies of 4-methyl-5-β-hydroxyethylthiazole kinase from Pyrococcus horikoshii OT3 (PhThiK)
The first crystal structure of 4-methyl-5-β-hydroxyethylthiazole kinase from an archaeon (P. horikoshii OT3) has been determined at 1.85 Å resolution. Comparative analyses of sequences and structures and modelling studies are presented.
PMCID: PMC2765881
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The structure of Staphylococcus aureus phosphopantetheine adenylyltransferase in complex with 3′-phosphoadenosine 5′-phosphosulfate reveals a new ligand-binding mode
The crystal structure of S. aureus phosphopantetheine adenylyltransferase in complex with 3′-phosphoadenosine 5′-phosphosulfate has been determined and reveals a new ligand-binding mode.
PMCID: PMC2765882
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High-resolution structure of human carbonic anhydrase II complexed with acetazolamide reveals insights into inhibitor drug design
The crystal structure of human carbonic anhydrase II (CA II) complexed with acetazolamide (AZM) has been determined at 1.1 Å resolution. The co-binding of AZM and glycerol in the active site demonstrate that an isozyme specific CA inhibitor may be developed.
PMCID: PMC2765883
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Crystallization Communications
Expression, purification and preliminary X-ray analysis of the Neisseria meningitidis outer membrane protein PorB
The major outer membrane protein PorB from N. meningitidis was crystallized in three crystal forms; the best X-ray diffraction data were collected to 2.3 Å resolution.
PMCID: PMC2765884
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Purification and crystallization of a putative transcriptional regulator of the benzoate oxidation pathway in Burkholderia xenovorans LB400
The X-ray diffraction and preliminary phasing of the putative transcriptional regulator Bxe_C0898 from B. xenovorans LB400 are reported.
PMCID: PMC2765885
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Expression, purification, crystallization and preliminary X-ray analysis of para-nitrophenol 4-monooxygenase from Pseudomonas putida DLL-E4
The crystallization and preliminary crystallographic analysis of a para-nitrophenol 4-monooxygenase PnpA from Pseudomonas putida DLL-E4 are presented.
PMCID: PMC2765886
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Crystallization and heavy-atom derivatization of StHsp14.0, a small heat-shock protein from Sulfolobus tokodaii
A variant of StHsp14.0, a small heat-shock protein from S. tokodaii, has been crystallized. Heavy-atom derivative crystals could be prepared by the cocrystallization method.
PMCID: PMC2765887
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Crystallization and preliminary X-ray diffraction analysis of crotoxin B from Crotalus durissus collilineatus venom
Crotoxin B is a basic phospholipase A2 found in the venom of C. durissus collilineatus and is one of the subunits which constitute the crotoxin. Here, we present the crystallization, X-ray diffraction data collection and molecular-replacement solution of the dimeric complex formed by two crotoxin B isoforms.
PMCID: PMC2765888
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Crystallization and preliminary X-ray analysis of mannosyl-3-phosphoglycerate synthase from Thermus thermophilus HB27
Mannosyl-3-phosphoglycerate synthase (MpgS) is a key enzyme in the biosynthesis of MG. Here, the purification, crystallization and preliminary crystallographic characterization of apo MpgS from Thermus thermophilus HB27 are reported.
PMCID: PMC2765889
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Crystallization and preliminary X-ray analysis of ZHE1, a hatching enzyme from the zebrafish Danio rerio
The hatching enzyme of zebrafish, ZHE1, was expressed, purified and crystallized using the hanging-drop vapour-diffusion method. The crystal belonged to space group P212121 and diffracted X-rays to a resolution of 1.14 Å.
PMCID: PMC2765890
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Crystallization and preliminary X-ray diffraction characterization of RpfF, a key DSF synthase from Stenotrophomonas maltophilia
Crystals of recombinant RpfF from S. maltophilia are tetragonal, space group P41212 or P43212, with unit-cell parameters a = 148.51, c = 122.82 Å, and diffract to 2.25 Å resolution.
PMCID: PMC2765891
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Crystallization and preliminary X-ray analysis of a d-Ala:d-Ser ligase associated with VanG-type vancomycin resistance
The VanG d-alanine:d-serine ligase was crystallized in complex with ADP and diffraction data were collected at 2.35 Å resolution.
PMCID: PMC2765892
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Purification, crystallization and preliminary X-ray diffraction studies of parakeet (Psittacula krameri) haemoglobin
Parakeet (Psittacula krameri) haemoglobin has been purified and crystallized under low salt buffered conditions. Preliminary analysis of the crystal that belonged to monoclinic system (C2) is reported.
PMCID: PMC2765893
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Crystallization and crystal-packing studies of Chlorella virus deoxyuridine triphosphatase
Algal Chlorella virus IL-3A deoxyuridine triphosphatase and its Glu81Ser plus Thr84Arg-mutated derivative, Mu-22, were crystallized using the hanging-drop vapor-diffusion method. Glycyl-seryl-tagged dUTPases yielded cubic and hexagonal crystals for IL-3A and Mu-22, respectively.
PMCID: PMC2765894
Expression, purification and preliminary crystallographic analysis of Pseudomonas aeruginosa RocR protein
P. aeruginosa RocR, an EAL-domain protein, has been expressed in E. coli, purified and crystallized. An X-ray diffraction data set was collected to a resolution of 2.50 Å.
PMCID: PMC2765895
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Crystallization and preliminary X-ray crystallographic studies of O-methyltransferase from Anabaena PCC 7120
The O-methyltransferase (OMT) from the Anabaena PCC 7120 has been overexpressed in a soluble form in E. coli, purified and crystallized. The crystals belonged to space group C2221 and diffracted to 2.4 Å resolution.
PMCID: PMC2765896
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Towards the structure of the C-terminal part of the S-layer protein SbsC
Various N-terminally truncated forms of the S-layer protein SbsC were crystallized and the form rSbsC(755–1099), which was obtained by unintentional in situ proteolysis, was used for preliminary structure determination.
PMCID: PMC2765897
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A novel noncovalent complex of chorismate mutase and DAHP synthase from Mycobacterium tuberculosis: protein purification, crystallization and X-ray diffraction analysis
Two shikimate-pathway enzymes from M. tuberculosis, the intracellular chorismate mutase (MtCM) and DAHP synthase (MtDS), were produced recombinantly and purified. MtCM was crystallized alone and in complex with MtDS and analyzed by X-ray diffraction.
PMCID: PMC2765898
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Preliminary X-ray crystallographic analysis of a nitric oxide-inducible lactate dehydrogenase from Staphylococcus aureus
A nitric oxide-inducible lactate dehydrogenase from S. aureus, Sa-LDH-1, has been cloned, expressed, purified and crystallized in space group C2, with unit-cell parameters a = 131.4, b = 74.4, c = 103.2 Å, β = 133.4°.
PMCID: PMC2765899
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Crystallization and preliminary X-ray diffraction characterization of an essential protein from Xanthomonas campestris that contains a noncanonical PilZ signature motif yet is critical for pathogenicity
An essential protein from the plant pathogen X. campestris pv. campestris (Xcc) that contains a noncanonical PilZ signature motif yet is critical for Xcc pathogenicity has been overexpressed in E. coli, purified and crystallized. The crystals diffracted to a resolution of 2.1 Å.
PMCID: PMC2765900
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Crystallization and preliminary X-ray diffraction analysis of the middle domain of Paip1
The crystallization of the putative MIF4G domain of Paip1 is described. The crystals belonged to the monoclinic space group P21 and diffracted X-rays to beyond 2.2 Å resolution.
PMCID: PMC2765901
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Cloning, purification and preliminary X-ray crystallographic analysis of a hypothetical protein, MJ0754, from Methanococcus jannaschii DSM 2661
In this study, two forms of MJ0754 from the archaeon M. jannaschii were overexpressed and crystallized. The crystal of MJ0754 belonged to the hexagonal space group P61 and diffracted to 3.1 Å resolution, while the crystal of MJ0754t belonged to the orthogonal space group C2221 and diffracted to 1.3 Å resolution using synchrotron radiation.
PMCID: PMC2765902
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Laboratory Communications
How to get the magic triangle and the MAD triangle into your protein crystal
The handling of the phasing tools I3C and B3C is described, emphasizing practical aspects such as the preparation of solutions and incorporation of the compounds into protein crystals.
PMCID: PMC2765903
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