|
|
Other Issues: Journal List
|
previous
|
next
|
latest
|
archive
Correspondence Effects of research tool patents on biotechnology innovation in a developing country: A case study of South Korea Kyung-Nam Kang, Tae-Kyu Ryu, and Yoon-Sik Lee PMCID: PMC2682799 Research article Genetic load and transgenic mitigating genes in transgenic Brassica rapa (field mustard) × Brassica napus (oilseed rape) hybrid populations Christy W Rose, Reginald J Millwood, Hong S Moon, Murali R Rao, Matthew D Halfhill, Paul L Raymer, Suzanne I Warwick, Hani Al-Ahmad, Jonathan Gressel, and C Neal Stewart, Jr PMCID: PMC2780409 Software Gene Composer: database software for protein construct design, codon engineering, and gene synthesis Don Lorimer, Amy Raymond, John Walchli, Mark Mixon, Adrienne Barrow, Ellen Wallace, Rena Grice, Alex Burgin, and Lance Stewart PMCID: PMC2681465 Research Articles Reporter gene-expressing bone marrow-derived stromal cells are immune-tolerated following implantation in the central nervous system of syngeneic immunocompetent mice Irene Bergwerf, Nathalie De Vocht, Bart Tambuyzer, Jacob Verschueren, Kristien Reekmans, Jasmijn Daans, Abdelilah Ibrahimi, Viggo Van Tendeloo, Shyama Chatterjee, Herman Goossens, Philippe G Jorens, Veerle Baekelandt, Dirk Ysebaert, Eric Van Marck, Zwi N Berneman, Annemie Van Der Linden, and Peter Ponsaerts PMCID: PMC2630974 Albumin fibrillization induces apoptosis via integrin/FAK/Akt pathway Chun-Yung Huang, Chi-Ming Liang, Chiao-Li Chu, and Shu-Mei Liang PMCID: PMC2630307 Gene therapy by electroporation for the treatment of chronic renal failure in companion animals Patricia A Brown, Angela M Bodles-Brakhop, Melissa A Pope, and Ruxandra Draghia-Akli PMCID: PMC2663557 Silica-based cationic bilayers as immunoadjuvants Nilton Lincopan, Mariana RA Santana, Eliana Faquim-Mauro, Maria Helena B da Costa, and Ana M Carmona-Ribeiro PMCID: PMC2647919 A compact phage display human scFv library for selection of antibodies to a wide variety of antigens Potjamas Pansri, Nanthnit Jaruseranee, Kuntalee Rangnoi, Peter Kristensen, and Montarop Yamabhai PMCID: PMC2642811 Detection of genetically modified organisms (GMOs) using isothermal amplification of target DNA sequences David Lee, Maurizio La Mura, Theo R Allnutt, and Wayne Powell PMCID: PMC2656497 In vitro drug release behavior from a novel thermosensitive composite hydrogel based on Pluronic f127 and poly(ethylene glycol)-poly(ε-caprolactone)-poly(ethylene glycol) copolymer Chang Yang Gong, Shuai Shi, Peng Wei Dong, Xiu Ling Zheng, Shao Zhi Fu, Gang Guo, Jing Liang Yang, Yu Quan Wei, and Zhi Yong Qian PMCID: PMC2654890 Isolation of osteogenic progenitors from human amniotic fluid using a single step culture protocol Ivana Antonucci, Irene Iezzi, Elisena Morizio, Filiberto Mastrangelo, Andrea Pantalone, Monica Mattioli-Belmonte, Antonio Gigante, Vincenzo Salini, Giuseppe Calabrese, Stefano Tetè, Giandomenico Palka, and Liborio Stuppia PMCID: PMC2654889 Noninvasive technique for measurement of heartbeat regularity in zebrafish (Danio rerio) embryos Po Kwok Chan, Chun Chi Lin, and Shuk Han Cheng PMCID: PMC2664803 Improving the functional expression of a Bacillus licheniformis laccase by random and site-directed mutagenesis Katja Koschorreck, Rolf D Schmid, and Vlada B Urlacher PMCID: PMC2653023 Incorporating double copies of a chromatin insulator into lentiviral vectors results in less viral integrants Troels T Nielsen, Johan Jakobsson, Nina Rosenqvist, and Cecilia Lundberg PMCID: PMC2651870 Addition of a sequence from α2-antiplasmin transforms human serum albumin into a blood clot component that speeds clot lysis William P Sheffield, Louise J Eltringham-Smith, Sharon Gataiance, and Varsha Bhakta PMCID: PMC2654442 The properties of bioengineered chondrocyte sheets for cartilage regeneration Genya Mitani, Masato Sato, Jeong IK Lee, Nagatoshi Kaneshiro, Miya Ishihara, Naoshi Ota, Mami Kokubo, Hideaki Sakai, Tetsutaro Kikuchi, and Joji Mochida PMCID: PMC2662823 A novel method for producing target cells and assessing cytotoxic T lymphocyte activity in outbred hosts Francesca Bonci, Elisa Zabogli, Francesca Conti, Antonio Merico, Giulia Freer, Mauro Bendinelli, and Mauro Pistello PMCID: PMC2662824 Chromato-panning: an efficient new mode of identifying suitable ligands from phage display libraries Wim Noppe, Fatima Plieva, Igor Yu Galaev, Hans Pottel, Hans Deckmyn, and Bo Mattiasson PMCID: PMC2667499 Viral and murine interleukin-10 are correctly processed and retain their biological activity when produced in tobacco Luisa Bortesi, Marzia Rossato, Flora Schuster, Nicole Raven, Johannes Stadlmann, Linda Avesani, Alberto Falorni, Flavia Bazzoni, Ralph Bock, Stefan Schillberg, and Mario Pezzotti PMCID: PMC2667500 Adenoviral vectors for highly selective gene expression in central serotonergic neurons reveal quantal characteristics of serotonin release in the rat brain Kheira Benzekhroufa, Beihui Liu, Feige Tang, Anja G Teschemacher, and Sergey Kasparov PMCID: PMC2672940 Practical and reliable FRET/FLIM pair of fluorescent proteins Dmitry Shcherbo, Ekaterina A Souslova, Joachim Goedhart, Tatyana V Chepurnykh, Anna Gaintzeva, Irina I Shemiakina, Theodorus WJ Gadella, Sergey Lukyanov, and Dmitriy M Chudakov PMCID: PMC2678114 Accumulation and processing of a recombinant protein designed as a cleavable fusion to the endogenous Rubisco LSU protein in Chlamydomonas chloroplast Machiko Muto, Ryan E Henry, and Stephen P Mayfield PMCID: PMC2671499 Folding machineries displayed on a cation-exchanger for the concerted refolding of cysteine- or proline-rich proteins Dae-Hee Lee, Sung-Gun Kim, Dae-Hyuk Kweon, and Jin-Ho Seo PMCID: PMC2676282 AC133+ progenitor cells as gene delivery vehicle and cellular probe in subcutaneous tumor models: a preliminary study Ali M Rad, ASM Iskander, Branislava Janic, Robert A Knight, Ali S Arbab, and Hamid Soltanian-Zadeh PMCID: PMC2669076 Vaccination with recombinant Boophilus annulatus Bm86 ortholog protein, Ba86, protects cattle against B. annulatus and B. microplus infestations Mario Canales, Consuelo Almazán, Victoria Naranjo, Frans Jongejan, and José de la Fuente PMCID: PMC2667501 PhiC31 integrase induces a DNA damage response and chromosomal rearrangements in human adult fibroblasts Jian Liu, Tina Skjørringe, Torben Gjetting, and Thomas G Jensen PMCID: PMC2682486 Noncytotoxic orange and red/green derivatives of DsRed-Express2 for whole-cell labeling Rita L Strack, Dibyendu Bhattacharyya, Benjamin S Glick, and Robert J Keenan PMCID: PMC2678115 Optimization of codon composition and regulatory elements for expression of human insulin like growth factor-1 in transgenic chloroplasts and evaluation of structural identity and function Henry Daniell, Gricel Ruiz, Bela Denes, Laurence Sandberg, and William Langridge PMCID: PMC2678116 Collagen matrices from sponge to nano: new perspectives for tissue engineering of skeletal muscle Justus P Beier, Dorothee Klumpp, Markus Rudisile, Roland Dersch, Joachim H Wendorff, Oliver Bleiziffer, Andreas Arkudas, Elias Polykandriotis, Raymund E Horch, and Ulrich Kneser PMCID: PMC2674407 A comparison of synthetic oligodeoxynucleotides, DNA fragments and AAV-1 for targeted episomal and chromosomal gene repair Xavier Leclerc, Olivier Danos, Daniel Scherman, and Antoine Kichler PMCID: PMC2676283 Combined protein construct and synthetic gene engineering for heterologous protein expression and crystallization using Gene Composer Amy Raymond, Scott Lovell, Don Lorimer, John Walchli, Mark Mixon, Ellen Wallace, Kaitlin Thompkins, Kimberly Archer, Alex Burgin, and Lance Stewart PMCID: PMC2680836 Acetylation of PAMAM dendrimers for cellular delivery of siRNA Carolyn L Waite, Sarah M Sparks, Kathryn E Uhrich, and Charles M Roth PMCID: PMC2679008 Efficient CPP-mediated Cre protein delivery to developing and adult CNS tissues Yorick Gitton, Lorenzo Tibaldi, Edmond Dupont, Giovanni Levi, and Alain Joliot PMCID: PMC2680837 A suite of recombinant luminescent bacterial strains for the quantification of bioavailable heavy metals and toxicity testing Angela Ivask, Taisia Rõlova, and Anne Kahru PMCID: PMC2685376 The CD-loop of PAI-2 (SERPINB2) is redundant in the targeting, inhibition and clearance of cell surface uPA activity Blake J Cochran, Lakshitha P Gunawardhana, Kara L Vine, Jodi A Lee, Sergei Lobov, and Marie Ranson PMCID: PMC2693429 Luciferase activity under direct ligand-dependent control of a muscarinic acetylcholine receptor Doreen Thor, Diana Le Duc, Rainer Strotmann, and Torsten Schöneberg PMCID: PMC2689208 Peroxicretion: a novel secretion pathway in the eukaryotic cell Cees MJ Sagt, Peter J ten Haaft, Ingeborg M Minneboo, Miranda P Hartog, Robbert A Damveld, Jan Metske van der Laan, Michiel Akeroyd, Thibaut J Wenzel, Francisca A Luesken, Marten Veenhuis, Ida van der Klei, and Johannes H de Winde PMCID: PMC2693430 A microarray-based method for the parallel analysis of genotypes and expression profiles of wood-forming tissues in Eucalyptus grandis Eugenia Barros, Carol-Ann van Staden, and Sabine Lezar PMCID: PMC2698882 High-titer preparation of Bombyx mori nucleopolyhedrovirus (BmNPV) displaying recombinant protein in silkworm larvae by size exclusion chromatography and its characterization Tatsuya Kato, Suganthi Lavender Manoha, Shigeyasu Tanaka, and Enoch Y Park PMCID: PMC2703641 Expression of endo-1, 4-beta-xylanase from Trichoderma reesei in Pichia pastoris and functional characterization of the produced enzyme Jun He, Bing Yu, Keying Zhang, Xuemei Ding, and Daiwen Chen PMCID: PMC2702311 Novel sequential ChIP and simplified basic ChIP protocols for promoter co-occupancy and target gene identification in human embryonic stem cells Ricardo B Medeiros, Kate J Papenfuss, Brian Hoium, Kristen Coley, Joy Jadrich, Saik-Kia Goh, Anuratha Elayaperumal, Julio E Herrera, Ernesto Resnik, and Hsiao-Tzu Ni PMCID: PMC2709612 Isolation of a human-like antibody fragment (scFv) that neutralizes ricin biological activity Thibaut Pelat, Michael Hust, Martha Hale, Marie-Paule Lefranc, Stefan Dübel, and Philippe Thullier PMCID: PMC2716335 A further insight into the biosorption mechanism of Pt(IV) by infrared spectrometry Zhongyu Lin, Ru Xue, Yiwen Ye, Jianhong Zheng, and Zhenling Xu PMCID: PMC2728714 Production of N-acetyl-D-neuraminic acid using two sequential enzymes overexpressed as double-tagged fusion proteins Tzu-Hsien Wang, Ying-Yin Chen, Hsin-Hung Pan, Feng-Pao Wang, Chung-Hsien Cheng, and Wen-Chien Lee PMCID: PMC2722590 A novel Poly(ε-caprolactone)-Pluronic-Poly(ε-caprolactone) grafted Polyethyleneimine(PCFC-g-PEI), Part 1, synthesis, cytotoxicity, and in vitro transfection study Shuai Shi, QingFa Guo, Bing Kan, ShaoZhi Fu, XiuHong Wang, ChangYang Gong, HongXin Deng, Feng Luo, Xia Zhao, YuQuan Wei, and ZhiYong Qian PMCID: PMC2717081 Gamma-phage lysin PlyG sequence-based synthetic peptides coupled with Qdot-nanocrystals are useful for developing detection methods for Bacillus anthracis by using its surrogates, B. anthracis-Sterne and B. cereus-4342 Shilpakala Sainathrao, Ketha V Krishna Mohan, and Chintamani Atreya PMCID: PMC2722591 Evaluation of cross-linked aggregates from purified Bacillus subtilis levansucrase mutants for transfructosylation reactions Maria Elena Ortiz-Soto, Enrique Rudiño-Piñera, Maria Elena Rodriguez-Alegria, and Agustin Lopez Munguia PMCID: PMC2724506 Efficient production of human bivalent and trivalent anti-MUC1 Fab-scFv antibodies in Pichia pastoris Steve Schoonooghe, Vladimir Kaigorodov, Monika Zawisza, Caroline Dumolyn, Jurgen Haustraete, Johan Grooten, and Nico Mertens PMCID: PMC2736937 Archaeosomes made of Halorubrum tebenquichense total polar lipids: a new source of adjuvancy Raul O Gonzalez, Leticia H Higa, Romina A Cutrullis, Marcos Bilen, Irma Morelli, Diana I Roncaglia, Ricardo S Corral, Maria Jose Morilla, Patricia B Petray, and Eder L Romero PMCID: PMC2739508 Transduction of the MPG-tagged fusion protein into mammalian cells and oocytes depends on amiloride-sensitive endocytic pathway So-Jung Kwon, Kyuyong Han, Suhyun Jung, Jong-Eun Lee, Seongsoon Park, Yong-Pil Cheon, and Hyunjung Jade Lim PMCID: PMC2748071 Directed evolution of a filamentous fungus for thermotolerance Eudes de Crecy, Stefan Jaronski, Benjamin Lyons, Thomas J Lyons, and Nemat O Keyhani PMCID: PMC2741439 Evaluation of the impact of single nucleotide polymorphisms and primer mismatches on quantitative PCR Brian Boyle, Nancy Dallaire, and John MacKay PMCID: PMC2741440 cMyc increases cell number through uncoupling of cell division from cell size in CHO cells Darrin Kuystermans and Mohamed Al-Rubeai PMCID: PMC2749834 New stably transfected bioluminescent cells expressing FLAG epitope-tagged estrogen receptors to study their chromatin recruitment Eric Badia, Aurélie Escande, Patrick Balaguer, Raphaël Métivier, and Vincent Cavailles PMCID: PMC2749030 A comparative study of recombinant and native frutalin binding to human prostate tissues Carla Oliveira, José A Teixeira, Fernando Schmitt, and Lucília Domingues PMCID: PMC2754448 Antibody expressing pea seeds as fodder for prevention of gastrointestinal parasitic infections in chickens Jana Zimmermann, Isolde Saalbach, Doreen Jahn, Martin Giersberg, Sigrun Haehnel, Julia Wedel, Jeanette Macek, Karen Zoufal, Gerhard Glünder, Dieter Falkenburg, and Sergey M Kipriyanov PMCID: PMC2755478 Integrating human stem cell expansion and neuronal differentiation in bioreactors Margarida Serra, Catarina Brito, Eunice M Costa, Marcos FQ Sousa, and Paula M Alves PMCID: PMC2759925 Thermochemotherapy effect of nanosized As2O3/Fe3O4 complex on experimental mouse tumors and its influence on the expression of CD44v6, VEGF-C and MMP-9 Yiqun Du, Dongsheng Zhang, Hui Liu, and Rensheng Lai PMCID: PMC2765420 Reduction of liver macrophage transduction by pseudotyping lentiviral vectors with a fusion envelope from Autographa californica GP64 and Sendai virus F2 domain David M Markusic, Niek P van Til, Johan K Hiralall, Ronald PJ Oude Elferink, and Jurgen Seppen PMCID: PMC2762966 Refinement of lentiviral vector for improved RNA processing and reduced rates of self inactivation repair Rachel M Koldej and Donald S Anson PMCID: PMC2765960 Regulated expression of a transgene introduced on an oriP/EBNA-1 PAC shuttle vector into human cells Hanne A Askautrud, Elisabet Gjernes, Gro L Størvold, Mona M Lindeberg, Jim Thorsen, Hans Prydz, and Eirik Frengen PMCID: PMC2770504 Novel immortalized human fetal liver cell line, cBAL111, has the potential to differentiate into functional hepatocytes Tanja Deurholt, Niek P van Til, Aniska A Chhatta, Lysbeth ten Bloemendaal, Ruth Schwartlander, Catherine Payne, John N Plevris, Igor M Sauer, Robert AFM Chamuleau, Ronald PJ Oude Elferink, Jurgen Seppen, and Ruurdtje Hoekstra PMCID: PMC2770505 Methodology Articles Bacterial artificial chromosomes improve recombinant protein production in mammalian cells Leander Blaas, Monica Musteanu, Robert Eferl, Anton Bauer, and Emilio Casanova PMCID: PMC2653024 Simplified production and concentration of HIV-1-based lentiviral vectors using HYPERFlask vessels and anion exchange membrane chromatography Robert H Kutner, Sharon Puthli, Michael P Marino, and Jakob Reiser PMCID: PMC2649911 A multi-Fc-species system for recombinant antibody production Sandrine Moutel, Ahmed El Marjou, Ole Vielemeyer, Clément Nizak, Philippe Benaroch, Stefan Dübel, and Franck Perez PMCID: PMC2654441 Real-time quantification of wild-type contaminants in glyphosate tolerant soybean Elena Battistini and Enrico Noli PMCID: PMC2656496 An optimised system for refolding of human glucose 6-phosphate dehydrogenase Xiao-Tao Wang and Paul C Engel PMCID: PMC2660318 A BAC-bacterial recombination method to generate physically linked multiple gene reporter DNA constructs Peter Maye, Mary Louise Stover, Yaling Liu, David W Rowe, Shiaoching Gong, and Alexander C Lichtler PMCID: PMC2662825 Simultaneous detection of mRNA and protein stem cell markers in live cells Won Jong Rhee and Gang Bao PMCID: PMC2682800 A modified and automated version of the 'Fluorimetric Detection of Alkaline DNA Unwinding' method to quantify formation and repair of DNA strand breaks María Moreno-Villanueva, Ragen Pfeiffer, Thilo Sindlinger, Alan Leake, Marcus Müller, Thomas BL Kirkwood, and Alexander Bürkle PMCID: PMC2679009 Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteins Antoine W Caron, Claire Nicolas, Bruno Gaillet, Ismaïla Ba, Maxime Pinard, Alain Garnier, Bernard Massie, and Rénald Gilbert PMCID: PMC2689207 RNAi in the cereal weevil Sitophilus spp: Systemic gene knockdown in the bacteriome tissue Agnès Vallier, Carole Vincent-Monégat, Anne Laurençon, and Abdelaziz Heddi PMCID: PMC2687439 Fluorescent labeling of NASBA amplified tmRNA molecules for microarray applications Ott Scheler, Barry Glynn, Sven Parkel, Priit Palta, Kadri Toome, Lauris Kaplinski, Maido Remm, Majella Maher, and Ants Kurg PMCID: PMC2685129 Generation of an external guide sequence library for a reverse genetic screen in Caenorhabditis elegans Qitao Yan, Rui Zhao, Wenlin Zheng, Changxin Yin, Bao Zhang, and Wenli Ma PMCID: PMC2696436 Expanding the multicolor capabilities of basic confocal microscopes by employing red and near-infrared quantum dot conjugates Lara M Kingeter and Brian C Schaefer PMCID: PMC2695445 Cost-effective HRMA pre-sequence typing of clone libraries; application to phage display selection Barry A Pepers, Menno H Schut, Rolf HAM Vossen, Gert-Jan B van Ommen, Johan T den Dunnen, and Willeke MC van Roon-Mom PMCID: PMC2694173 Integrated application of uniform design and least-squares support vector machines to transfection optimization Jin-Shui Pan, Mei-Zhu Hong, Qi-Feng Zhou, Jia-Yan Cai, Hua-Zhen Wang, Lin-Kai Luo, De-Qiang Yang, Jing Dong, Hua-Xiu Shi, and Jian-Lin Ren PMCID: PMC2701423 Construction of siRNA/miRNA expression vectors based on a one-step PCR process Jun Xu, Jie Qiong Zeng, Gang Wan, Gui Bin Hu, Hong Yan, and Li Xin Ma PMCID: PMC2700792 Interfering ribonucleic acids that suppress expression of multiple unrelated genes Toby Passioura, Mary M Gozar, Amber Goodchild, Andrew King, Greg M Arndt, Michael Poidinger, Donald J Birkett, and Laurent P Rivory PMCID: PMC2706242 Cell-free protein synthesis energized by slowly-metabolized maltodextrin Yiran Wang and Y-H Percival Zhang PMCID: PMC2716334 Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies Oded Edelheit, Aaron Hanukoglu, and Israel Hanukoglu PMCID: PMC2711942 Stable nuclear transformation of Gonium pectorale Kai Lerche and Armin Hallmann PMCID: PMC2720962 Efficient, chemoselective synthesis of immunomicelles using single-domain antibodies with a C-terminal thioester Sanne WA Reulen, Ingrid van Baal, Jos MH Raats, and Maarten Merkx PMCID: PMC2719619 Efficient generation of long-distance conditional alleles using recombineering and a dual selection strategy in replicate plates David Voehringer, Davina Wu, Hong-Erh Liang, and Richard M Locksley PMCID: PMC2724507 High quality protein microarray using in situ protein purification Keehwan Kwon, Carissa Grose, Rembert Pieper, Gagan A Pandya, Robert D Fleischmann, and Scott N Peterson PMCID: PMC2746808 Monodispersity of recombinant Cre recombinase correlates with its effectiveness in vivo Paola Capasso, Marisa Aliprandi, Giuseppe Ossolengo, Frank Edenhofer, and Ario de Marco PMCID: PMC2755479 A combination of transposable elements and magnetic cell sorting provides a very efficient transgenesis system for chicken primary erythroid progenitors Camila Mejia-Pous, José Viñuelas, Claudine Faure, Joanna Koszela, Koichi Kawakami, Yoshiko Takahashi, and Olivier Gandrillon PMCID: PMC2753566 MALS: an efficient strategy for multiple site-directed mutagenesis employing a combination of DNA amplification, ligation and suppression PCR Alexey A Fushan and Dennis T Drayna PMCID: PMC2759926 Characterization of unknown genetic modifications using high throughput sequencing and computational subtraction Torstein Tengs, Haibo Zhang, Arne Holst-Jensen, Jon Bohlin, Melinka A Butenko, Anja Bråthen Kristoffersen, Hilde-Gunn Opsahl Sorteberg, and Knut G Berdal PMCID: PMC2764706 Authentication scheme for routine verification of genetically similar laboratory colonies: a trial with Anopheles gambiae Elien E Wilkins, Paula L Marcet, Alice C Sutcliffe, and Paul I Howell PMCID: PMC2772846 Other Issues: Journal List
|
previous
|
next
|
latest
|
archive
|
