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Volume 10;  2010
Research articles
Characterization of proton production and consumption associated with microbial metabolism
Karthikeyan Srinivasan and Radhakrishnan Mahadevan
BMC Biotechnol. 2010; 10: 2. Published online 2010 January 20. doi: 10.1186/1472-6750-10-2.
PMCID: PMC2831035
Senescent vs. non-senescent cells in the human annulus in vivo: Cell harvest with laser capture microdissection and gene expression studies with microarray analysis
Helen E Gruber, Gretchen L Hoelscher, Jane A Ingram, Natalia Zinchenko, and Edward N Hanley, Jr
BMC Biotechnol. 2010; 10: 5. Published online 2010 January 28. doi: 10.1186/1472-6750-10-5.
PMCID: PMC2828399
Heterologous expression of leader-less pga gene in Pichia pastoris: intracellular production of prokaryotic enzyme
Helena Marešová, Zdena Marková, Renáta Valešová, Jan Sklenář, and Pavel Kyslík
BMC Biotechnol. 2010; 10: 7. Published online 2010 February 3. doi: 10.1186/1472-6750-10-7.
PMCID: PMC2845550
Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA
Kristin S Gunnarsen, Elin Lunde, Per E Kristiansen, Bjarne Bogen, Inger Sandlie, and Geir Å Løset
BMC Biotechnol. 2010; 10: 8. Published online 2010 February 3. doi: 10.1186/1472-6750-10-8.
PMCID: PMC2834602
Comparative transfection of DNA into primary and transformed mammalian cells from different lineages
Rosalie Maurisse, David De Semir, Hamid Emamekhoo, Babak Bedayat, Alireza Abdolmohammadi, Hooman Parsi, and Dieter C Gruenert
BMC Biotechnol. 2010; 10: 9. Published online 2010 February 8. doi: 10.1186/1472-6750-10-9.
PMCID: PMC2830169
High-throughput detection of mutations responsible for childhood hearing loss using resequencing microarrays
Prachi Kothiyal, Stephanie Cox, Jonathan Ebert, Ammar Husami, Margaret A Kenna, John H Greinwald, Bruce J Aronow, and Heidi L Rehm
BMC Biotechnol. 2010; 10: 10. Published online 2010 February 10. doi: 10.1186/1472-6750-10-10.
PMCID: PMC2841091
Protein microarray: sensitive and effective immunodetection for drug residues
Li Zhong, Wei Zhang, Cindy Zer, Kun Ge, Xu Gao, and Kemp H Kernstine
BMC Biotechnol. 2010; 10: 12. Published online 2010 February 16. doi: 10.1186/1472-6750-10-12.
PMCID: PMC2827365
Broad spectrum microarray for fingerprint-based bacterial species identification
Frédérique Pasquer, Cosima Pelludat, Brion Duffy, and Jürg E Frey
BMC Biotechnol. 2010; 10: 13. Published online 2010 February 17. doi: 10.1186/1472-6750-10-13.
PMCID: PMC2830168
High-level expression and purification of soluble recombinant FGF21 protein by SUMO fusion in Escherichia coli
Huiyan Wang, Yechen Xiao, Lianjun Fu, Hongxin Zhao, Yaofang Zhang, Xiaoshan Wan, Yuxia Qin, Yadong Huang, Hongchang Gao, and Xiaokun Li
BMC Biotechnol. 2010; 10: 14. Published online 2010 February 17. doi: 10.1186/1472-6750-10-14.
PMCID: PMC2831817
Characterization of a molecular switch system that regulates gene expression in mammalian cells through a small molecule
Jennifer L Taylor, Priyanka Rohatgi, H Trent Spencer, Donald F Doyle, and Bahareh Azizi
BMC Biotechnol. 2010; 10: 15. Published online 2010 February 18. doi: 10.1186/1472-6750-10-15.
PMCID: PMC2831033
PhiC31 recombination system demonstrates heritable germinal transmission of site-specific excision from the Arabidopsis genome
James G Thomson, Ronald Chan, Roger Thilmony, Yuan-Yeu Yau, and David W Ow
BMC Biotechnol. 2010; 10: 17. Published online 2010 February 23. doi: 10.1186/1472-6750-10-17.
PMCID: PMC2837860
Heterologous expression of a tannic acid-inducible laccase3 of Cryphonectria parasitica in Saccharomyces cerevisiae
Jung-Mi Kim, Seung-Moon Park, and Dae-Hyuk Kim
BMC Biotechnol. 2010; 10: 18. Published online 2010 February 24. doi: 10.1186/1472-6750-10-18.
PMCID: PMC2839966
Anti-tumor therapy with macroencapsulated endostatin producer cells
Danielle B Rodrigues, Roger Chammas, Natália V Malavasi, Patrícia LN da Costa, Rosa M Chura-Chambi, Keli N Balduino, and Ligia Morganti
BMC Biotechnol. 2010; 10: 19. Published online 2010 March 2. doi: 10.1186/1472-6750-10-19.
PMCID: PMC2845092
pEPito: a significantly improved non-viral episomal expression vector for mammalian cells
Rudolf Haase, Orestis Argyros, Suet-Ping Wong, Richard P Harbottle, Hans J Lipps, Manfred Ogris, Terese Magnusson, Maria G Vizoso Pinto, Jürgen Haas, and Armin Baiker
BMC Biotechnol. 2010; 10: 20. Published online 2010 March 15. doi: 10.1186/1472-6750-10-20.
PMCID: PMC2847955
Yeast functional screen to identify genetic determinants capable of conferring abiotic stress tolerance in Jatropha curcas
Nalini Eswaran, Sriram Parameswaran, Balaji Sathram, Bhagyam Anantharaman, Raja Krishna Kumar G, and Sudhakar Johnson Tangirala
BMC Biotechnol. 2010; 10: 23. Published online 2010 March 20. doi: 10.1186/1472-6750-10-23.
PMCID: PMC2851662
Phenotypic analysis of images of zebrafish treated with Alzheimer's γ-secretase inhibitors
Dilyara Arslanova, Ting Yang, Xiaoyin Xu, Stephen T Wong, Corinne E Augelli-Szafran, and Weiming Xia
BMC Biotechnol. 2010; 10: 24. Published online 2010 March 22. doi: 10.1186/1472-6750-10-24.
PMCID: PMC2851663
A proteomic study of cMyc improvement of CHO culture
Darrin Kuystermans, Michael J Dunn, and Mohamed Al-Rubeai
BMC Biotechnol. 2010; 10: 25. Published online 2010 March 22. doi: 10.1186/1472-6750-10-25.
PMCID: PMC2859402
Use of the piggyBac transposon to create HIV-1 gag transgenic insect cell lines for continuous VLP production
Alisson G Lynch, Fiona Tanzer, Malcolm J Fraser, Enid G Shephard, Anna-Lise Williamson, and Edward P Rybicki
BMC Biotechnol. 2010; 10: 30. Published online 2010 March 31. doi: 10.1186/1472-6750-10-30.
PMCID: PMC2853493
Generation of anti-TLR2 intrabody mediating inhibition of macrophage surface TLR2 expression and TLR2-driven cell activation
Carsten J Kirschning, Stefan Dreher, Björn Maaß, Sylvia Fichte, Jutta Schade, Mario Köster, Andreas Noack, Werner Lindenmaier, Hermann Wagner, and Thomas Böldicke
BMC Biotechnol. 2010; 10: 31. Published online 2010 April 13. doi: 10.1186/1472-6750-10-31.
PMCID: PMC2873280
Production of recombinant cholesterol oxidase containing covalently bound FAD in Escherichia coli
Federica Volontè, Loredano Pollegioni, Gianluca Molla, Luca Frattini, Flavia Marinelli, and Luciano Piubelli
BMC Biotechnol. 2010; 10: 33. Published online 2010 April 21. doi: 10.1186/1472-6750-10-33.
PMCID: PMC2890692
Detection of tmRNA molecules on microarrays at low temperatures using helper oligonucleotides
Lauris Kaplinski, Ott Scheler, Sven Parkel, Priit Palta, Kadri Toome, Ants Kurg, and Maido Remm
BMC Biotechnol. 2010; 10: 34. Published online 2010 April 28. doi: 10.1186/1472-6750-10-34.
PMCID: PMC2873282
Heterogeneous detection of circulating tumor cells in patients with colorectal cancer by immunomagnetic enrichment using different EpCAM-specific antibodies
Dalibor Antolovic, Luis Galindo, Anina Carstens, Nuh Rahbari, Markus W Büchler, Jürgen Weitz, and Moritz Koch
BMC Biotechnol. 2010; 10: 35. Published online 2010 April 28. doi: 10.1186/1472-6750-10-35.
PMCID: PMC2868015
Degradation or excretion of quantum dots in mouse embryonic stem cells
Qing Meng Pi, Wen Jie Zhang, Guang Dong Zhou, Wei Liu, and Yilin Cao
BMC Biotechnol. 2010; 10: 36. Published online 2010 May 6. doi: 10.1186/1472-6750-10-36.
PMCID: PMC2876065
Exploitation of the interaction of measles virus fusogenic envelope proteins with the surface receptor CD46 on human cells for microcell-mediated chromosome transfer
Motonobu Katoh, Yasuhiro Kazuki, Kanako Kazuki, Naoyo Kajitani, Masato Takiguchi, Yuji Nakayama, Takafumi Nakamura, and Mitsuo Oshimura
BMC Biotechnol. 2010; 10: 37. Published online 2010 May 6. doi: 10.1186/1472-6750-10-37.
PMCID: PMC2874513
Microporation is a valuable transfection method for efficient gene delivery into human umbilical cord blood-derived mesenchymal stem cells
Jung Yeon Lim, Sun Hwa Park, Chang Hyun Jeong, Ji Hyeon Oh, Seong Muk Kim, Chung Hun Ryu, Soon A Park, Jae Geun Ahn, Wonil Oh, Sin-Soo Jeun, and Jong Wook Chang
BMC Biotechnol. 2010; 10: 38. Published online 2010 May 13. doi: 10.1186/1472-6750-10-38.
PMCID: PMC2883955
The production of fluorescent transgenic trout to study in vitro myogenic cell differentiation
Jean-Charles Gabillard, Cécile Rallière, Nathalie Sabin, and Pierre-Yves Rescan
BMC Biotechnol. 2010; 10: 39. Published online 2010 May 17. doi: 10.1186/1472-6750-10-39.
PMCID: PMC2887378
Hydrocarbon phenotyping of algal species using pyrolysis-gas chromatography mass spectrometry
Dinesh K Barupal, Tobias Kind, Shankar L Kothari, Do Yup Lee, and Oliver Fiehn
BMC Biotechnol. 2010; 10: 40. Published online 2010 May 21. doi: 10.1186/1472-6750-10-40.
PMCID: PMC2883956
Research Articles
Stable expression and phenotypic impact of attacin E transgene in orchard grown apple trees over a 12 year period
Ewa Borejsza-Wysocka, John L Norelli, Herb S Aldwinckle, and Mickael Malnoy
BMC Biotechnol. 2010; 10: 41. Published online 2010 June 3. doi: 10.1186/1472-6750-10-41.
PMCID: PMC2910661
α-Amylase inhibitor-1 gene from Phaseolus vulgaris expressed in Coffea arabica plants inhibits α-amylases from the coffee berry borer pest
Aulus EAD Barbosa, Érika VS Albuquerque, Maria CM Silva, Djair SL Souza, Osmundo B Oliveira-Neto, Arnubio Valencia, Thales L Rocha, and Maria F Grossi-de-Sa
BMC Biotechnol. 2010; 10: 44. Published online 2010 June 17. doi: 10.1186/1472-6750-10-44.
PMCID: PMC2914071
Susceptible cell lines for the production of porcine reproductive and respiratory syndrome virus by stable transfection of sialoadhesin and CD163
Iris Delrue, Hanne Van Gorp, Jan Van Doorsselaere, Peter L Delputte, and Hans J Nauwynck
BMC Biotechnol. 2010; 10: 48. Published online 2010 June 29. doi: 10.1186/1472-6750-10-48.
PMCID: PMC2908558
A process optimization for bio-catalytic production of substituted catechols (3-nitrocatechol and 3-methylcatechol
Dhan Prakash, Janmejay Pandey, Bhupendra N Tiwary, and Rakesh K Jain
BMC Biotechnol. 2010; 10: 49. Published online 2010 June 30. doi: 10.1186/1472-6750-10-49.
PMCID: PMC2906425
Exobiopolymer production of Ophiocordyceps dipterigena BCC 2073: optimization, production in bioreactor and characterization
Kanokarn Kocharin, Pranee Rachathewee, Jean-Jacques Sanglier, and Wai Prathumpai
BMC Biotechnol. 2010; 10: 51. Published online 2010 July 12. doi: 10.1186/1472-6750-10-51.
PMCID: PMC2912784
Effect of codon optimization and subcellular targeting on Toxoplasma gondii antigen SAG1 expression in tobacco leaves to use in subcutaneous and oral immunization in mice
Melina Laguía-Becher, Valentina Martín, Mauricio Kraemer, Mariana Corigliano, María L Yacono, Alejandra Goldman, and Marina Clemente
BMC Biotechnol. 2010; 10: 52. Published online 2010 July 15. doi: 10.1186/1472-6750-10-52.
PMCID: PMC2920232
SIVsm Tat, Rev, and Nef1: functional characteristics of r-GV internalization on isotypes, cytokines, and intracellular degradation
Marinko Sremac and Elizabeth S Stuart
BMC Biotechnol. 2010; 10: 54. Published online 2010 July 19. doi: 10.1186/1472-6750-10-54.
PMCID: PMC2916889
Testing the interaction between analytical modules: an example with Roundup Ready® soybean line GTS 40-3-2
Gianni Bellocchi, Marzia De Giacomo, Nicoletta Foti, Marco Mazzara, Eleonora Palmaccio, Cristian Savini, Chiara Di Domenicantonio, Roberta Onori, and Guy Van den Eede
BMC Biotechnol. 2010; 10: 55. Published online 2010 August 5. doi: 10.1186/1472-6750-10-55.
PMCID: PMC2927498
Moisture-induced solid state instabilities in α-chymotrypsin and their reduction through chemical glycosylation
Giselle M Flores-Fernández, Miraida Pagán, Mariangely Almenas, Ricardo J Solá, and Kai Griebenow
BMC Biotechnol. 2010; 10: 57. Published online 2010 August 9. doi: 10.1186/1472-6750-10-57.
PMCID: PMC2924255
Novel strong tissue specific promoter for gene expression in human germ cells
Denis Kuzmin, Elena Gogvadze, Roman Kholodenko, Dawid P Grzela, Maxim Mityaev, Tatyana Vinogradova, Eugene Kopantzev, Galina Malakhova, Maria Suntsova, Dmitry Sokov, Zoltán Ivics, and Anton Buzdin
BMC Biotechnol. 2010; 10: 58. Published online 2010 August 17. doi: 10.1186/1472-6750-10-58.
PMCID: PMC2929213
Methodology Articles
Functional genomics tool: Gene silencing in Ixodes scapularis eggs and nymphs by electroporated dsRNA
Shahid Karim, Emily Troiano, and Thomas N Mather
BMC Biotechnol. 2010; 10: 1. Published online 2010 January 14. doi: 10.1186/1472-6750-10-1.
PMCID: PMC2823657
Real-time monitoring of circadian clock oscillations in primary cultures of mammalian cells using Tol2 transposon-mediated gene transfer strategy
Kazuhiro Yagita, Iori Yamanaka, Noriaki Emoto, Koichi Kawakami, and Shoichi Shimada
BMC Biotechnol. 2010; 10: 3. Published online 2010 January 22. doi: 10.1186/1472-6750-10-3.
PMCID: PMC2823658
A novel FRET pair for detection of parallel DNA triplexes by the LightCycler
Uffe V Schneider, Jette K Severinsen, Imrich Géci, Limei M Okkels, Nina Jøhnk, Nikolaj D Mikkelsen, Teena Klinge, Erik B Pedersen, Henrik Westh, and Gorm Lisby
BMC Biotechnol. 2010; 10: 4. Published online 2010 January 27. doi: 10.1186/1472-6750-10-4.
PMCID: PMC2823659
A new approach to dual-color two-photon microscopy with fluorescent proteins
Shane E Tillo, Thomas E Hughes, Nikolay S Makarov, Aleks Rebane, and Mikhail Drobizhev
BMC Biotechnol. 2010; 10: 6. Published online 2010 February 2. doi: 10.1186/1472-6750-10-6.
PMCID: PMC2831818
A versatile polyacrylamide gel electrophoresis based sulfotransferase assay
Zhengliang L Wu, Cheryl M Ethen, Sara Larson, Brittany Prather, and Weiping Jiang
BMC Biotechnol. 2010; 10: 11. Published online 2010 February 10. doi: 10.1186/1472-6750-10-11.
PMCID: PMC2834601
Immunohistochemical detection of transgene expression in the brain using small epitope tags
Evy Lobbestael, Veerle Reumers, Abdelilah Ibrahimi, Kirsten Paesen, Irina Thiry, Rik Gijsbers, Chris Van den Haute, Zeger Debyser, Veerle Baekelandt, and Jean-Marc Taymans
BMC Biotechnol. 2010; 10: 16. Published online 2010 February 18. doi: 10.1186/1472-6750-10-16.
PMCID: PMC2831034
A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering
Morten HH Nørholm
BMC Biotechnol. 2010; 10: 21. Published online 2010 March 16. doi: 10.1186/1472-6750-10-21.
PMCID: PMC2847956
Replication methods and tools in high-throughput cultivation processes - recognizing potential variations of growth and product formation by on-line monitoring
Robert Huber, Thomas G Palmen, Nadine Ryk, Anne-Kathrin Hillmer, Karina Luft, Frank Kensy, and Jochen Büchs
BMC Biotechnol. 2010; 10: 22. Published online 2010 March 16. doi: 10.1186/1472-6750-10-22.
PMCID: PMC2847957
Promoter knock-in: a novel rational method for the fine tuning of genes
Marjan De Mey, Jo Maertens, Sarah Boogmans, Wim K Soetaert, Erick J Vandamme, Raymond Cunin, and Maria R Foulquié-Moreno
BMC Biotechnol. 2010; 10: 26. Published online 2010 March 24. doi: 10.1186/1472-6750-10-26.
PMCID: PMC2858092
Escherichia coli MW005: lambda Red-mediated recombineering and copy-number induction of oriV-equipped constructs in a single host
Marcel Westenberg, Sophie Bamps, Helen Soedling, Ian A Hope, and Colin T Dolphin
BMC Biotechnol. 2010; 10: 27. Published online 2010 March 29. doi: 10.1186/1472-6750-10-27.
PMCID: PMC2864197
Efficient gene delivery and silencing of mouse and human pancreatic islets
Bruno Lefebvre, Brigitte Vandewalle, Justine Longue, Ericka Moerman, Bruno Lukowiak, Valery Gmyr, Kathrin Maedler, Julie Kerr-conte, and François Pattou
BMC Biotechnol. 2010; 10: 28. Published online 2010 March 30. doi: 10.1186/1472-6750-10-28.
PMCID: PMC2853492
Comparative analysis of right element mutant lox sites on recombination efficiency in embryonic stem cells
Kimi Araki, Yuka Okada, Masatake Araki, and Ken-ichi Yamamura
BMC Biotechnol. 2010; 10: 29. Published online 2010 March 31. doi: 10.1186/1472-6750-10-29.
PMCID: PMC2865440
A simple fluorescence based assay for quantification of human immunodeficiency virus particle release
Johannes Hermle, Maria Anders, Anke-Mareil Heuser, and Barbara Müller
BMC Biotechnol. 2010; 10: 32. Published online 2010 April 20. doi: 10.1186/1472-6750-10-32.
PMCID: PMC2873281
Biotinylated-sortase self-cleavage purification (BISOP) method for cell-free produced proteins
Satoko Matsunaga, Kazuhiro Matsuoka, Kouhei Shimizu, Yaeta Endo, and Tatsuya Sawasaki
BMC Biotechnol. 2010; 10: 42. Published online 2010 June 4. doi: 10.1186/1472-6750-10-42.
PMCID: PMC2901362
Accurate non-invasive image-based cytotoxicity assays for cultured cells
Patricia Marqués-Gallego, Hans den Dulk, Claude Backendorf, Jaap Brouwer, Jan Reedijk, and Julian F Burke
BMC Biotechnol. 2010; 10: 43. Published online 2010 June 17. doi: 10.1186/1472-6750-10-43.
PMCID: PMC2906423
Real-time detection of viable microorganisms by intracellular phototautomerism
Remco Kort, Andreas Nocker, Alie de Kat Angelino-Bart, Sjaak van Veen, Herman Verheij, Frank Schuren, and Roy Montijn
BMC Biotechnol. 2010; 10: 45. Published online 2010 June 18. doi: 10.1186/1472-6750-10-45.
PMCID: PMC2906424
A modified fluorimetric host cell reactivation assay to determine the repair capacity of primary keratinocytes, melanocytes and fibroblasts
Katharina Burger, Katja Matt, Nicole Kieser, Daniel Gebhard, and Jörg Bergemann
BMC Biotechnol. 2010; 10: 46. Published online 2010 June 22. doi: 10.1186/1472-6750-10-46.
PMCID: PMC2900224
Optimization and analysis of a quantitative real-time PCR-based technique to determine microRNA expression in formalin-fixed paraffin-embedded samples
Rashmi S Goswami, Levi Waldron, Jerry Machado, Nilva K Cervigne, Wei Xu, Patricia P Reis, Denis J Bailey, Igor Jurisica, Michael R Crump, and Suzanne Kamel-Reid
BMC Biotechnol. 2010; 10: 47. Published online 2010 June 23. doi: 10.1186/1472-6750-10-47.
PMCID: PMC2902407
Ao38, a new cell line from eggs of the black witch moth, Ascalapha odorata (Lepidoptera: Noctuidae), is permissive for AcMNPV infection and produces high levels of recombinant proteins
Yoshifumi Hashimoto, Sheng Zhang, and Gary W Blissard
BMC Biotechnol. 2010; 10: 50. Published online 2010 July 5. doi: 10.1186/1472-6750-10-50.
PMCID: PMC2906426
Using quantitative real-time PCR to detect chimeras in transgenic tobacco and apricot and to monitor their dissociation
Mohamed Faize, Lydia Faize, and Lorenzo Burgos
BMC Biotechnol. 2010; 10: 53. Published online 2010 July 16. doi: 10.1186/1472-6750-10-53.
PMCID: PMC2912785
Automated seamless DNA co-transformation cloning with direct expression vectors applying positive or negative insert selection
Natacha Olieric, Melanie Kuchen, Sandro Wagen, Marion Sauter, Stephanie Crone, Sonia Edmondson, Daniel Frey, Christian Ostermeier, Michel O Steinmetz, and Rolf Jaussi
BMC Biotechnol. 2010; 10: 56. Published online 2010 August 9. doi: 10.1186/1472-6750-10-56.
PMCID: PMC2924254
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