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Research articles Characterization of proton production and consumption associated with microbial metabolism Karthikeyan Srinivasan and Radhakrishnan Mahadevan PMCID: PMC2831035 Senescent vs. non-senescent cells in the human annulus in vivo: Cell harvest with laser capture microdissection and gene expression studies with microarray analysis Helen E Gruber, Gretchen L Hoelscher, Jane A Ingram, Natalia Zinchenko, and Edward N Hanley, Jr PMCID: PMC2828399 Heterologous expression of leader-less pga gene in Pichia pastoris: intracellular production of prokaryotic enzyme Helena Marešová, Zdena Marková, Renáta Valešová, Jan Sklenář, and Pavel Kyslík PMCID: PMC2845550 Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA Kristin S Gunnarsen, Elin Lunde, Per E Kristiansen, Bjarne Bogen, Inger Sandlie, and Geir Å Løset PMCID: PMC2834602 Comparative transfection of DNA into primary and transformed mammalian cells from different lineages Rosalie Maurisse, David De Semir, Hamid Emamekhoo, Babak Bedayat, Alireza Abdolmohammadi, Hooman Parsi, and Dieter C Gruenert PMCID: PMC2830169 High-throughput detection of mutations responsible for childhood hearing loss using resequencing microarrays Prachi Kothiyal, Stephanie Cox, Jonathan Ebert, Ammar Husami, Margaret A Kenna, John H Greinwald, Bruce J Aronow, and Heidi L Rehm PMCID: PMC2841091 Protein microarray: sensitive and effective immunodetection for drug residues Li Zhong, Wei Zhang, Cindy Zer, Kun Ge, Xu Gao, and Kemp H Kernstine PMCID: PMC2827365 Broad spectrum microarray for fingerprint-based bacterial species identification Frédérique Pasquer, Cosima Pelludat, Brion Duffy, and Jürg E Frey PMCID: PMC2830168 High-level expression and purification of soluble recombinant FGF21 protein by SUMO fusion in Escherichia coli Huiyan Wang, Yechen Xiao, Lianjun Fu, Hongxin Zhao, Yaofang Zhang, Xiaoshan Wan, Yuxia Qin, Yadong Huang, Hongchang Gao, and Xiaokun Li PMCID: PMC2831817 Characterization of a molecular switch system that regulates gene expression in mammalian cells through a small molecule Jennifer L Taylor, Priyanka Rohatgi, H Trent Spencer, Donald F Doyle, and Bahareh Azizi PMCID: PMC2831033 PhiC31 recombination system demonstrates heritable germinal transmission of site-specific excision from the Arabidopsis genome James G Thomson, Ronald Chan, Roger Thilmony, Yuan-Yeu Yau, and David W Ow PMCID: PMC2837860 Heterologous expression of a tannic acid-inducible laccase3 of Cryphonectria parasitica in Saccharomyces cerevisiae Jung-Mi Kim, Seung-Moon Park, and Dae-Hyuk Kim PMCID: PMC2839966 Anti-tumor therapy with macroencapsulated endostatin producer cells Danielle B Rodrigues, Roger Chammas, Natália V Malavasi, Patrícia LN da Costa, Rosa M Chura-Chambi, Keli N Balduino, and Ligia Morganti PMCID: PMC2845092 pEPito: a significantly improved non-viral episomal expression vector for mammalian cells Rudolf Haase, Orestis Argyros, Suet-Ping Wong, Richard P Harbottle, Hans J Lipps, Manfred Ogris, Terese Magnusson, Maria G Vizoso Pinto, Jürgen Haas, and Armin Baiker PMCID: PMC2847955 Yeast functional screen to identify genetic determinants capable of conferring abiotic stress tolerance in Jatropha curcas Nalini Eswaran, Sriram Parameswaran, Balaji Sathram, Bhagyam Anantharaman, Raja Krishna Kumar G, and Sudhakar Johnson Tangirala PMCID: PMC2851662 Phenotypic analysis of images of zebrafish treated with Alzheimer's γ-secretase inhibitors Dilyara Arslanova, Ting Yang, Xiaoyin Xu, Stephen T Wong, Corinne E Augelli-Szafran, and Weiming Xia PMCID: PMC2851663 A proteomic study of cMyc improvement of CHO culture Darrin Kuystermans, Michael J Dunn, and Mohamed Al-Rubeai PMCID: PMC2859402 Use of the piggyBac transposon to create HIV-1 gag transgenic insect cell lines for continuous VLP production Alisson G Lynch, Fiona Tanzer, Malcolm J Fraser, Enid G Shephard, Anna-Lise Williamson, and Edward P Rybicki PMCID: PMC2853493 Generation of anti-TLR2 intrabody mediating inhibition of macrophage surface TLR2 expression and TLR2-driven cell activation Carsten J Kirschning, Stefan Dreher, Björn Maaß, Sylvia Fichte, Jutta Schade, Mario Köster, Andreas Noack, Werner Lindenmaier, Hermann Wagner, and Thomas Böldicke PMCID: PMC2873280 Production of recombinant cholesterol oxidase containing covalently bound FAD in Escherichia coli Federica Volontè, Loredano Pollegioni, Gianluca Molla, Luca Frattini, Flavia Marinelli, and Luciano Piubelli PMCID: PMC2890692 Detection of tmRNA molecules on microarrays at low temperatures using helper oligonucleotides Lauris Kaplinski, Ott Scheler, Sven Parkel, Priit Palta, Kadri Toome, Ants Kurg, and Maido Remm PMCID: PMC2873282 Heterogeneous detection of circulating tumor cells in patients with colorectal cancer by immunomagnetic enrichment using different EpCAM-specific antibodies Dalibor Antolovic, Luis Galindo, Anina Carstens, Nuh Rahbari, Markus W Büchler, Jürgen Weitz, and Moritz Koch PMCID: PMC2868015 Degradation or excretion of quantum dots in mouse embryonic stem cells Qing Meng Pi, Wen Jie Zhang, Guang Dong Zhou, Wei Liu, and Yilin Cao PMCID: PMC2876065 Exploitation of the interaction of measles virus fusogenic envelope proteins with the surface receptor CD46 on human cells for microcell-mediated chromosome transfer Motonobu Katoh, Yasuhiro Kazuki, Kanako Kazuki, Naoyo Kajitani, Masato Takiguchi, Yuji Nakayama, Takafumi Nakamura, and Mitsuo Oshimura PMCID: PMC2874513 Microporation is a valuable transfection method for efficient gene delivery into human umbilical cord blood-derived mesenchymal stem cells Jung Yeon Lim, Sun Hwa Park, Chang Hyun Jeong, Ji Hyeon Oh, Seong Muk Kim, Chung Hun Ryu, Soon A Park, Jae Geun Ahn, Wonil Oh, Sin-Soo Jeun, and Jong Wook Chang PMCID: PMC2883955 The production of fluorescent transgenic trout to study in vitro myogenic cell differentiation Jean-Charles Gabillard, Cécile Rallière, Nathalie Sabin, and Pierre-Yves Rescan PMCID: PMC2887378 Hydrocarbon phenotyping of algal species using pyrolysis-gas chromatography mass spectrometry Dinesh K Barupal, Tobias Kind, Shankar L Kothari, Do Yup Lee, and Oliver Fiehn PMCID: PMC2883956 Research Articles Stable expression and phenotypic impact of attacin E transgene in orchard grown apple trees over a 12 year period Ewa Borejsza-Wysocka, John L Norelli, Herb S Aldwinckle, and Mickael Malnoy PMCID: PMC2910661 α-Amylase inhibitor-1 gene from Phaseolus vulgaris expressed in Coffea arabica plants inhibits α-amylases from the coffee berry borer pest Aulus EAD Barbosa, Érika VS Albuquerque, Maria CM Silva, Djair SL Souza, Osmundo B Oliveira-Neto, Arnubio Valencia, Thales L Rocha, and Maria F Grossi-de-Sa PMCID: PMC2914071 Susceptible cell lines for the production of porcine reproductive and respiratory syndrome virus by stable transfection of sialoadhesin and CD163 Iris Delrue, Hanne Van Gorp, Jan Van Doorsselaere, Peter L Delputte, and Hans J Nauwynck PMCID: PMC2908558 A process optimization for bio-catalytic production of substituted catechols (3-nitrocatechol and 3-methylcatechol Dhan Prakash, Janmejay Pandey, Bhupendra N Tiwary, and Rakesh K Jain PMCID: PMC2906425 Exobiopolymer production of Ophiocordyceps dipterigena BCC 2073: optimization, production in bioreactor and characterization Kanokarn Kocharin, Pranee Rachathewee, Jean-Jacques Sanglier, and Wai Prathumpai PMCID: PMC2912784 Effect of codon optimization and subcellular targeting on Toxoplasma gondii antigen SAG1 expression in tobacco leaves to use in subcutaneous and oral immunization in mice Melina Laguía-Becher, Valentina Martín, Mauricio Kraemer, Mariana Corigliano, María L Yacono, Alejandra Goldman, and Marina Clemente PMCID: PMC2920232 SIVsm Tat, Rev, and Nef1: functional characteristics of r-GV internalization on isotypes, cytokines, and intracellular degradation Marinko Sremac and Elizabeth S Stuart PMCID: PMC2916889 Testing the interaction between analytical modules: an example with Roundup Ready® soybean line GTS 40-3-2 Gianni Bellocchi, Marzia De Giacomo, Nicoletta Foti, Marco Mazzara, Eleonora Palmaccio, Cristian Savini, Chiara Di Domenicantonio, Roberta Onori, and Guy Van den Eede PMCID: PMC2927498 Moisture-induced solid state instabilities in α-chymotrypsin and their reduction through chemical glycosylation Giselle M Flores-Fernández, Miraida Pagán, Mariangely Almenas, Ricardo J Solá, and Kai Griebenow PMCID: PMC2924255 Novel strong tissue specific promoter for gene expression in human germ cells Denis Kuzmin, Elena Gogvadze, Roman Kholodenko, Dawid P Grzela, Maxim Mityaev, Tatyana Vinogradova, Eugene Kopantzev, Galina Malakhova, Maria Suntsova, Dmitry Sokov, Zoltán Ivics, and Anton Buzdin PMCID: PMC2929213 Methodology Articles Functional genomics tool: Gene silencing in Ixodes scapularis eggs and nymphs by electroporated dsRNA Shahid Karim, Emily Troiano, and Thomas N Mather PMCID: PMC2823657 Real-time monitoring of circadian clock oscillations in primary cultures of mammalian cells using Tol2 transposon-mediated gene transfer strategy Kazuhiro Yagita, Iori Yamanaka, Noriaki Emoto, Koichi Kawakami, and Shoichi Shimada PMCID: PMC2823658 A novel FRET pair for detection of parallel DNA triplexes by the LightCycler Uffe V Schneider, Jette K Severinsen, Imrich Géci, Limei M Okkels, Nina Jøhnk, Nikolaj D Mikkelsen, Teena Klinge, Erik B Pedersen, Henrik Westh, and Gorm Lisby PMCID: PMC2823659 A new approach to dual-color two-photon microscopy with fluorescent proteins Shane E Tillo, Thomas E Hughes, Nikolay S Makarov, Aleks Rebane, and Mikhail Drobizhev PMCID: PMC2831818 A versatile polyacrylamide gel electrophoresis based sulfotransferase assay Zhengliang L Wu, Cheryl M Ethen, Sara Larson, Brittany Prather, and Weiping Jiang PMCID: PMC2834601 Immunohistochemical detection of transgene expression in the brain using small epitope tags Evy Lobbestael, Veerle Reumers, Abdelilah Ibrahimi, Kirsten Paesen, Irina Thiry, Rik Gijsbers, Chris Van den Haute, Zeger Debyser, Veerle Baekelandt, and Jean-Marc Taymans PMCID: PMC2831034 A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering Morten HH Nørholm PMCID: PMC2847956 Replication methods and tools in high-throughput cultivation processes - recognizing potential variations of growth and product formation by on-line monitoring Robert Huber, Thomas G Palmen, Nadine Ryk, Anne-Kathrin Hillmer, Karina Luft, Frank Kensy, and Jochen Büchs PMCID: PMC2847957 Promoter knock-in: a novel rational method for the fine tuning of genes Marjan De Mey, Jo Maertens, Sarah Boogmans, Wim K Soetaert, Erick J Vandamme, Raymond Cunin, and Maria R Foulquié-Moreno PMCID: PMC2858092 Escherichia coli MW005: lambda Red-mediated recombineering and copy-number induction of oriV-equipped constructs in a single host Marcel Westenberg, Sophie Bamps, Helen Soedling, Ian A Hope, and Colin T Dolphin PMCID: PMC2864197 Efficient gene delivery and silencing of mouse and human pancreatic islets Bruno Lefebvre, Brigitte Vandewalle, Justine Longue, Ericka Moerman, Bruno Lukowiak, Valery Gmyr, Kathrin Maedler, Julie Kerr-conte, and François Pattou PMCID: PMC2853492 Comparative analysis of right element mutant lox sites on recombination efficiency in embryonic stem cells Kimi Araki, Yuka Okada, Masatake Araki, and Ken-ichi Yamamura PMCID: PMC2865440 A simple fluorescence based assay for quantification of human immunodeficiency virus particle release Johannes Hermle, Maria Anders, Anke-Mareil Heuser, and Barbara Müller PMCID: PMC2873281 Biotinylated-sortase self-cleavage purification (BISOP) method for cell-free produced proteins Satoko Matsunaga, Kazuhiro Matsuoka, Kouhei Shimizu, Yaeta Endo, and Tatsuya Sawasaki PMCID: PMC2901362 Accurate non-invasive image-based cytotoxicity assays for cultured cells Patricia Marqués-Gallego, Hans den Dulk, Claude Backendorf, Jaap Brouwer, Jan Reedijk, and Julian F Burke PMCID: PMC2906423 Real-time detection of viable microorganisms by intracellular phototautomerism Remco Kort, Andreas Nocker, Alie de Kat Angelino-Bart, Sjaak van Veen, Herman Verheij, Frank Schuren, and Roy Montijn PMCID: PMC2906424 A modified fluorimetric host cell reactivation assay to determine the repair capacity of primary keratinocytes, melanocytes and fibroblasts Katharina Burger, Katja Matt, Nicole Kieser, Daniel Gebhard, and Jörg Bergemann PMCID: PMC2900224 Optimization and analysis of a quantitative real-time PCR-based technique to determine microRNA expression in formalin-fixed paraffin-embedded samples Rashmi S Goswami, Levi Waldron, Jerry Machado, Nilva K Cervigne, Wei Xu, Patricia P Reis, Denis J Bailey, Igor Jurisica, Michael R Crump, and Suzanne Kamel-Reid PMCID: PMC2902407 Ao38, a new cell line from eggs of the black witch moth, Ascalapha odorata (Lepidoptera: Noctuidae), is permissive for AcMNPV infection and produces high levels of recombinant proteins Yoshifumi Hashimoto, Sheng Zhang, and Gary W Blissard PMCID: PMC2906426 Using quantitative real-time PCR to detect chimeras in transgenic tobacco and apricot and to monitor their dissociation Mohamed Faize, Lydia Faize, and Lorenzo Burgos PMCID: PMC2912785 Automated seamless DNA co-transformation cloning with direct expression vectors applying positive or negative insert selection Natacha Olieric, Melanie Kuchen, Sandro Wagen, Marion Sauter, Stephanie Crone, Sonia Edmondson, Daniel Frey, Christian Ostermeier, Michel O Steinmetz, and Rolf Jaussi PMCID: PMC2924254 Other Issues: Journal List
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