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Commentary Towards universal systems for recombinant gene expression Hans Peter Sørensen PMCID: PMC2876075 Reviews The path to next generation biofuels: successes and challenges in the era of synthetic biology Clementina Dellomonaco, Fabio Fava, and Ramon Gonzalez PMCID: PMC2817670 Production of recombinant proteins in E. coli by the heat inducible expression system based on the phage lambda pL and/or pR promoters Norma A Valdez-Cruz, Luis Caspeta, Néstor O Pérez, Octavio T Ramírez, and Mauricio A Trujillo-Roldán PMCID: PMC2848208 Technical Notes Sandwich ELISA for quantitative detection of human collagen prolyl 4-hydroxylase Ekaterina Osmekhina, Antje Neubauer, Katharina Klinzing, Johanna Myllyharju, and Peter Neubauer PMCID: PMC2895579 Automated assay for screening the enzymatic release of reducing sugars from micronized biomass David Navarro, Marie Couturier, Gabriela Ghizzi Damasceno da Silva, Jean-Guy Berrin, Xavier Rouau, Marcel Asther, and Christophe Bignon PMCID: PMC2919459 Editorial Nanotechnology, bionanotechnology and microbial cell factories Antonio Villaverde PMCID: PMC2916890 Research Metabolic engineering of Agrobacterium sp. strain ATCC 31749 for production of an α-Gal epitope Anne M Ruffing and Rachel R Chen PMCID: PMC2818619 Expression and display of UreA of Helicobacter acinonychis on the surface of Bacillus subtilis spores Krzysztof Hinc, Rachele Isticato, Marcin Dembek, Joanna Karczewska, Adam Iwanicki, Grażyna Peszyńska-Sularz, Maurilio De Felice, Michał Obuchowski, and Ezio Ricca PMCID: PMC2841587 Heterologous expression of glucose oxidase in the yeast Kluyveromyces marxianus Saul N Rocha, José Abrahão-Neto, María E Cerdán, María I González-Siso, and Andreas K Gombert PMCID: PMC2817671 Isolation, characterization and heterologous expression of a novel chitosanase from Janthinobacterium sp. strain 4239 Mads G Johnsen, Ole C Hansen, and Peter Stougaard PMCID: PMC2835661 Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into Streptomyces ansochromogenes lead to an improved nikkomycin production Guojian Liao, Jine Li, Lei Li, Haihua Yang, Yuqing Tian, and Huarong Tan PMCID: PMC2817672 Cloning of the Zygosaccharomyces bailii GAS1 homologue and effect of cell wall engineering on protein secretory phenotype Simone Passolunghi, Luca Riboldi, Laura Dato, Danilo Porro, and Paola Branduardi PMCID: PMC2825207 Metabolic regulation of Escherichia coli and its gdhA, glnL, gltB, D mutants under different carbon and nitrogen limitations in the continuous culture Rahul Kumar and Kazuyuki Shimizu PMCID: PMC2827463 Reduction of oxidative cellular damage by overexpression of the thioredoxin TRX2 gene improves yield and quality of wine yeast dry active biomass Rocío Gómez-Pastor, Roberto Pérez-Torrado, Elisa Cabiscol, Joaquim Ros, and Emilia Matallana PMCID: PMC2835662 Characterization of two diesel fuel degrading microbial consortia enriched from a non acclimated, complex source of microorganisms Giulio Zanaroli, Sara Di Toro, Daniela Todaro, Giovanna C Varese, Antonio Bertolotto, and Fabio Fava PMCID: PMC2830956 A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures Mirja Krause, Kaisa Ukkonen, Tatu Haataja, Maria Ruottinen, Tuomo Glumoff, Antje Neubauer, Peter Neubauer, and Antti Vasala PMCID: PMC2841585 Bacterial diversity and reductive dehalogenase redundancy in a 1,2-dichloroethane-degrading bacterial consortium enriched from a contaminated aquifer Massimo Marzorati, Annalisa Balloi, Francesca de Ferra, Lorenzo Corallo, Giovanna Carpani, Lieven Wittebolle, Willy Verstraete, and Daniele Daffonchio PMCID: PMC2834577 Biosynthesis of 2-hydroxyisobutyric acid (2-HIBA) from renewable carbon Thore Rohwerder and Roland H Müller PMCID: PMC2847961 Evaluation of different expression systems for the heterologous expression of pyranose 2-oxidase from Trametes multicolor in E. coli Oliver Spadiut, Gerald Posch, Roland Ludwig, Dietmar Haltrich, and Clemens K Peterbauer PMCID: PMC2841586 Effect of HXT1 and HXT7 hexose transporter overexpression on wild-type and lactic acid producing Saccharomyces cerevisiae cells Giorgia Rossi, Michael Sauer, Danilo Porro, and Paola Branduardi PMCID: PMC2848207 Fermentation of mixed glucose-xylose substrates by engineered strains of Saccharomyces cerevisiae: role of the coenzyme specificity of xylose reductase, and effect of glucose on xylose utilization Stefan Krahulec, Barbara Petschacher, Michael Wallner, Karin Longus, Mario Klimacek, and Bernd Nidetzky PMCID: PMC2847541 Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast Eva García-Ruiz, Diana Maté, Antonio Ballesteros, Angel T Martinez, and Miguel Alcalde PMCID: PMC2847542 Influence of production process design on inclusion bodies protein: the case of an Antarctic flavohemoglobin Ermenegilda Parrilli, Maria Giuliani, Gennaro Marino, and Maria Luisa Tutino PMCID: PMC2857821 Efficient recombinant expression and secretion of a thermostable GH26 mannan endo-1,4-β-mannosidase from Bacillus licheniformis in Escherichia coli Chomphunuch Songsiriritthigul, Bancha Buranabanyat, Dietmar Haltrich, and Montarop Yamabhai PMCID: PMC2868798 Metabolic engineering for the production of shikimic acid in an evolved Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system Adelfo Escalante, Rocío Calderón, Araceli Valdivia, Ramón de Anda, Georgina Hernández, Octavio T Ramírez, Guillermo Gosset, and Francisco Bolívar PMCID: PMC2873404 Co-expression of Skp and FkpA chaperones improves cell viability and alters the global expression of stress response genes during scFvD1.3 production Dave Siak-Wei Ow, Denis Yong-Xiang Lim, Peter Morin Nissom, Andrea Camattari, and Victor Vai-Tak Wong PMCID: PMC2868799 The intra- and extracellular proteome of Aspergillus niger growing on defined medium with xylose or maltose as carbon substrate Xin Lu, Jibin Sun, Manfred Nimtz, Josef Wissing, An-Ping Zeng, and Ursula Rinas PMCID: PMC2874515 Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst Sandra Abad, Jozef Nahalka, Gabriele Bergler, S Alison Arnold, Robert Speight, Ian Fotheringham, Bernd Nidetzky, and Anton Glieder PMCID: PMC2873405 Enhanced fluorescent properties of an OmpT site deleted mutant of Green Fluorescent Protein Shardul S Salunkhe, Veena A Raiker, Sachin Rewanwar, Prakash Kotwal, Avijeet Kumar, and Sriram Padmanabhan PMCID: PMC2868801 Microbial polyhydroxyalkanote synthesis repression protein PhaR as an affinity tag for recombinant protein purification Shuang Zhang, Zhi Hui Wang, and Guo Qiang Chen PMCID: PMC2873406 A highly efficient pipeline for protein expression in Leishmania tarentolae using infrared fluorescence protein as marker Hakan Dortay and Bernd Mueller-Roeber PMCID: PMC2874516 A novel prokaryotic vector for identification and selection of recombinants: Direct use of the vector for expression studies in E. coli Sampali Banerjee, Jitendra Kumar, Anjali Apte-Deshpande, and Sriram Padmanabhan PMCID: PMC2882348 Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin Chandrasekhar Gurramkonda, Sulena Polez, Natasa Skoko, Ahmad Adnan, Thomas Gäbel, Dipti Chugh, Sathyamangalam Swaminathan, Navin Khanna, Sergio Tisminetzky, and Ursula Rinas PMCID: PMC2882349 Cocktail δ-integration: a novel method to construct cellulolytic enzyme expression ratio-optimized yeast strains Ryosuke Yamada, Naho Taniguchi, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, and Akihiko Kondo PMCID: PMC2876996 Expression of an extremely acidic β-1,4-glucanase from thermoacidophilic Alicyclobacillus sp. A4 in Pichia pastoris is improved by truncating the gene sequence Yingguo Bai, Jianshe Wang, Zhifang Zhang, Pengjun Shi, Huiying Luo, Huoqing Huang, Chunliang Luo, and Bin Yao PMCID: PMC2880286 Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin Anna Zanfardino, Odile F Restaino, Eugenio Notomista, Donatella Cimini, Chiara Schiraldi, Mario De Rosa, Maurilio De Felice, and Mario Varcamonti PMCID: PMC2889854 Novel approach of high cell density recombinant bioprocess development: Optimisation and scale-up from microlitre to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures Juozas Šiurkus, Johanna Panula-Perälä, Uwe Horn, Mario Kraft, Renata Rimšeliene, and Peter Neubauer PMCID: PMC2890543 Quantitative evaluation of yeast's requirement for glycerol formation in very high ethanol performance fed-batch process Julien Pagliardini, Georg Hubmann, Carine Bideaux, Sandrine Alfenore, Elke Nevoigt, and Stéphane E Guillouet PMCID: PMC2887396 Production and purification of staphylococcal nuclease in Lactococcus lactis using a new expression-secretion system and a pH-regulated mini-reactor Nicolas Trémillon, Nicolas Issaly, Julien Mozo, Thomas Duvignau, Hervé Ginisty, Eric Devic, and Isabelle Poquet PMCID: PMC2887397 Reduced evolvability of Escherichia coli MDS42, an IS-less cellular chassis for molecular and synthetic biology applications Kinga Umenhoffer, Tamás Fehér, Gabriella Balikó, Ferhan Ayaydin, János Pósfai, Frederick R Blattner, and György Pósfai PMCID: PMC2891674 High yield expression of an AHL-lactonase from Bacillus sp. B546 in Pichia pastoris and its application to reduce Aeromonas hydrophila mortality in aquaculture Ruidong Chen, Zhigang Zhou, Yanan Cao, Yingguo Bai, and Bin Yao PMCID: PMC2881887 PGM2 overexpression improves anaerobic galactose fermentation in Saccharomyces cerevisiae Rosa Garcia Sanchez, Bärbel Hahn-Hägerdal, and Marie F Gorwa-Grauslund PMCID: PMC2896927 Quality control of inclusion bodies in Escherichia coli Britta Jürgen, Antje Breitenstein, Vlada Urlacher, Knut Büttner, Hongying Lin, Michael Hecker, Thomas Schweder, and Peter Neubauer PMCID: PMC2893105 High cell density cultivation and recombinant protein production with Escherichia coli in a rocking-motion-type bioreactor Julia Glazyrina, Eva-Maria Materne, Thomas Dreher, Dirk Storm, Stefan Junne, Thorsten Adams, Gerhard Greller, and Peter Neubauer PMCID: PMC2891675 Molecular cloning and biochemical characterization of a novel erythrose reductase from Candida magnoliae JH110 Dae-Hee Lee, Ye-Ji Lee, Yeon-Woo Ryu, and Jin-Ho Seo PMCID: PMC2902421 Two heterologously expressed Planobispora rosea proteins cooperatively induce Streptomyces lividans thiostrepton uptake and storage from the extracellular medium Anna Giardina, Rosa Alduina, Elvira Gottardi, Valentina Di Caro, Roderich D Süssmuth, and Anna M Puglia PMCID: PMC2893106 MARINE-EXPRESS: taking advantage of high throughput cloning and expression strategies for the post-genomic analysis of marine organisms Agnès Groisillier, Cécile Hervé, Alexandra Jeudy, Etienne Rebuffet, Pierre F Pluchon, Yann Chevolot, Didier Flament, Claire Geslin, Isabel M Morgado, Déborah Power, Margherita Branno, Hervé Moreau, Gurvan Michel, Catherine Boyen, and Mirjam Czjzek PMCID: PMC2897777 Hyaluronic acid production by Streptococcus zooepidemicus in marine by-products media from mussel processing wastewaters and tuna peptone viscera José A Vázquez, María I Montemayor, Javier Fraguas, and Miguel A Murado PMCID: PMC2901256 Increasing cell biomass in Saccharomyces cerevisiae increases recombinant protein yield: the use of a respiratory strain as a microbial cell factory Cecilia Ferndahl, Nicklas Bonander, Christel Logez, Renaud Wagner, Lena Gustafsson, Christer Larsson, Kristina Hedfalk, Richard AJ Darby, and Roslyn M Bill PMCID: PMC2901257 The HAC1 gene from Pichia pastoris: characterization and effect of its overexpression on the production of secreted, surface displayed and membrane proteins Mouna Guerfal, Stefan Ryckaert, Pieter P Jacobs, Paul Ameloot, Kathleen Van Craenenbroeck, Riet Derycke, and Nico Callewaert PMCID: PMC2905327 Genome-scale metabolic reconstruction and in silico analysis of methylotrophic yeast Pichia pastoris for strain improvement Bevan KS Chung, Suresh Selvarasu, Camattari Andrea, Jimyoung Ryu, Hyeokweon Lee, Jungoh Ahn, Hongweon Lee, and Dong-Yup Lee PMCID: PMC2908565 Whole-cell fungal transformation of precursors into dyes Jolanta Polak and Anna Jarosz-Wilkołazka PMCID: PMC2913955 Aerobic biogenesis of selenium nanospheres by Bacillus cereus isolated from coalmine soil Soniya Dhanjal and Swaranjit Singh Cameotra PMCID: PMC2909957 Production of biohydrogen by recombinant expression of [NiFe]-hydrogenase 1 in Escherichia coli Jaoon YH Kim, Byung Hoon Jo, and Hyung Joon Cha PMCID: PMC2908566 Development of a Bacillus subtilis expression system using the improved Pglv promoter Yang M Ming, Zhang W Wei, Chen Y Lin, and Gong Y Sheng PMCID: PMC2908567 Global expression studies in baker's yeast reveal target genes for the improvement of industrially-relevant traits: the cases of CAF16 and ORC2 Roberto Pérez-Torrado, Joaquín Panadero, María José Hernández-López, José Antonio Prieto, and Francisca Randez-Gil PMCID: PMC2912791 Characterisation of recombinant pyranose oxidase from the cultivated mycorrhizal basidiomycete Lyophyllum shimeji (hon-shimeji) Clara Salaheddin, Yoshimitsu Takakura, Masako Tsunashima, Barbara Stranzinger, Oliver Spadiut, Montarop Yamabhai, Clemens K Peterbauer, and Dietmar Haltrich PMCID: PMC2914677 An antibiotic, heavy metal resistant and halotolerant Bacillus cereus SIU1 and its thermoalkaline protease Sanjay K Singh, Vinayak R Tripathi, Rakesh K Jain, Surendra Vikram, and Satyendra K Garg PMCID: PMC2914678 New method for selection of hydrogen peroxide adapted bifidobacteria cells using continuous culture and immobilized cell technology Valeria Mozzetti , Franck Grattepanche, Déborah Moine , Bernard Berger , Enea Rezzonico , Leo Meile , Fabrizio Arigoni , and Christophe Lacroix PMCID: PMC2922086 Production of glycoprotein vaccines in Escherichia coli Julian Ihssen, Michael Kowarik, Sandro Dilettoso, Cyril Tanner, Michael Wacker, and Linda Thöny-Meyer PMCID: PMC2927510 Correction Gene cloning and characterization of a novel esterase from activated sludge metagenome Tao Zhang and WenJun Han PMCID: PMC2868800 Corrects: . 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