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J Clin Microbiol. Jan 2000; 38(1): 467.
PMCID: PMC88756

Detection of Bordetella holmesii Using Bordetella pertussis IS481 PCR Assay

After our manuscript comparing the performance of PCR, culture, and direct fluorescent-antibody testing for Bordetella pertussis (3) went to press, we obtained six isolates of Bordetella holmesii for the purpose of testing with our B. pertussis IS481 PCR assay. This request for isolates was prompted by the recent report by Yih et al. (6) on the isolation of B. holmesii from nasopharyngeal (NP) specimens from patients with pertussis-like cough. Previously, B. holmesii has been associated with septicemia in immunocompromised patients (5) and was isolated from the sputum of patients with respiratory failure (4). Yih et al. isolated B. holmesii infrequently from NP specimens during their evaluation (0.26% positivity rate, compared to 6.6% positivity rate for B. pertussis) (6).

B. holmesii isolates (kindly provided by H. George, Massachusetts Department of Public Health) were grown on Trypticase soy agar plates containing 5% sheep blood for 48 h at 35°C. Colony picks were suspended in sterile water and were tested with the B. pertussis PCR assay as previously described (3). Initially, the quantity of organisms added to PCRs was not determined, but it was estimated to be at least 100 to 1,000 CFU per reaction. All six B. holmesii isolates generated strong positive results with the B. pertussis IS481 PCR assay. To evaluate the limit of detection, suspensions of two B. holmesii isolates were prepared, and cell concentrations were estimated based on McFarland turbidimetric standards. Aliquots of serial dilutions were tested with the B. pertussis PCR assay. The analytical sensitivity ranged from 0.06 to 0.3 CFU per PCR, which is similar to the sensitivity we previously reported for B. pertussis (3).

B. pertussis PCR assays that target the IS481 repetitive element may cross-react with B. holmesii. Our PCR assay utilizes previously described primers and probe. The sequences of the upstream and downstream primers were previously described by Glare et al. (1) and He et al. (2), respectively. The sequence of our capture probe is identical to that of the downstream primer used by Glare et al. (1). We have not determined whether actual NP specimens from patients infected or colonized with B. holmesii would generate a positive result with our B. pertussis PCR test. We have not isolated B. holmesii from NP swab specimens in our laboratory. However, our Regan-Lowe transport medium contains 40 μg of cephalexin per ml, to which B. holmesii is reportedly susceptible (E. Mazengia, H. George, E. Silva, and J. Peppe, Abstr. 99th Gen. Meet. Am. Soc. Microbiol. 1999, abstr. A-113, p. 24, 1999). It is possible that some of the patients in our earlier study (3) who were positive by PCR alone were infected or colonized with B. holmesii and not with B. pertussis. However, if the B. holmesii prevalence in our study were similar to that reported in Massachusetts, the number of positive specimens would be very low.

The clinical and public health significance of B. holmesii as a respiratory pathogen in otherwise healthy individuals remains to be determined. In fact, it has yet to be proven conclusively that B. holmesii causes respiratory disease. Nonetheless, these data suggest that B. holmesii may confound B. pertussis PCR assays that target IS481, requiring redefinition of performance characteristics or modification of assay methods.

REFERENCES

1. Glare E M, Paton J C, Premier R R, Lawrence A J, Nisbet I T. Analysis of a repetitive DNA sequence from Bordetella pertussis and its application to the diagnosis of pertussis using the polymerase chain reaction. J Clin Microbiol. 1990;28:1982–1987. [PMC free article] [PubMed]
2. He Q, Mertsola J, Soini H, Skurnik M, Ruuskanen O, Viljanen M K. Comparison of polymerase chain reaction with culture and enzyme immunoassay for diagnosis of pertussis. J Clin Microbiol. 1993;31:642–645. [PMC free article] [PubMed]
3. Loeffelholz M J, Thompson C J, Long K S, Gilchrist M J R. Comparison of PCR, culture, and direct fluorescent-antibody testing for detection of Bordetella pertussis. J Clin Microbiol. 1999;37:2872–2876. [PMC free article] [PubMed]
4. Tang Y W, Hopkins M K, Kolbert C P, Hartley P A, Severance P J, Persing D H. Bordetella holmesii-like organisms associated with septicemia, endocarditis, and respiratory failure. Clin Infect Dis. 1998;26:389–392. [PubMed]
5. Weyant R S, Hollis D G, Weaver R E, Amin M F M, Steigerwalt A G, O'Connor S P, Whitney A M, Daneshvar M I, Moss C W, Brenner D J. Bordetella holmesii sp. nov., a new gram-negative species associated with septicemia. J Clin Microbiol. 1995;33:1–7. [PMC free article] [PubMed]
6. Yih W K, Silva E A, Ida J, Harrington N, Lett S M, George H. Bordetella holmesii-like organisms isolated from Massachusetts patients with pertussis-like symptoms. Emerg Inf Dis. 1999;5:441–443. [PMC free article] [PubMed]

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