• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of embojLink to Publisher's site
EMBO J. Dec 20, 1984; 3(13): 3231–3237.
PMCID: PMC557842

Comparison of the entire genomes of bovine leukemia virus and human T-cell leukemia virus and characterization of their unidentified open reading frames.

Abstract

We have compared the sequence of the entire genomes of bovine leukemia virus (BLV) and human T-cell leukemia virus type I (HTLV-I). Both the gag and pol genes show overall strong homologies indicating the close evolutionary relationship of the two retroviruses. However, a surface glycoprotein portion of the env gene shows no appreciable homology, which probably reflects a difference in their host ranges. The 3' end portion of the BLV genome (designated as pXBL) contains an unidentified long open reading frame that has a typical protein-coding property. The potential product of this open reading frame may be a glycoprotein of approximately 40 000 daltons. We note that its amino acid sequence shows low but appreciable homology, especially in its N-terminal quarter, to that of the HTLV-I counterpart (pX product), and we thus suggest that BLV pXBL and HTLV-I pX have diverged from a common ancestral gene. It is tentatively concluded that both the putative pXBL and pX products are respectively produced from a spliced mRNA.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Copeland TD, Morgan MA, Oroszlan S. Complete amino acid sequence of the nucleic acid-binding protein of bovine leukemia virus. FEBS Lett. 1983 May 30;156(1):37–40. [PubMed]
  • Copeland TD, Oroszlan S, Kalyanaraman VS, Sarngadharan MG, Gallo RC. Complete amino acid sequence of human T-cell leukemia virus structural protein p15. FEBS Lett. 1983 Oct 17;162(2):390–395. [PubMed]
  • Deschamps J, Kettmann R, Burny A. Experiments with cloned complete tumor-derived bovine leukemia virus information prove that the virus is totally exogenous to its target animal species. J Virol. 1981 Nov;40(2):605–609. [PMC free article] [PubMed]
  • Elder JH, Mullins JI. Nucleotide sequence of the envelope gene of Gardner-Arnstein feline leukemia virus B reveals unique sequence homologies with a murine mink cell focus-forming virus. J Virol. 1983 Jun;46(3):871–880. [PMC free article] [PubMed]
  • Fukui K, Noma T, Takeuchi K, Kobayashi N, Hatanaka M, Honjo T. Origin of adult T-cell leukemia virus. Implication for its zoonosis. Mol Biol Med. 1983 Nov;1(4):447–456. [PubMed]
  • Grandgenett DP, Vora AC, Schiff RD. A 32,000-dalton nucleic acid-binding protein from avian retravirus cores possesses DNA endonuclease activity. Virology. 1978 Aug;89(1):119–132. [PubMed]
  • Grandgenett DP, Golomb M, Vora AC. Activation of an Mg2+-dependent DNA endonuclease of avian myeloblastosis virus alpha beta DNA polymerase by in vitro proteolytic cleavage. J Virol. 1980 Jan;33(1):264–271. [PMC free article] [PubMed]
  • Haseltine WA, Sodroski J, Patarca R, Briggs D, Perkins D, Wong-Staal F. Structure of 3' terminal region of type II human T lymphotropic virus: evidence for new coding region. Science. 1984 Jul 27;225(4660):419–421. [PubMed]
  • Hattori S, Imagawa K, Shimizu F, Hashimura E, Seiki M, Yoshida M. Identification of envelope glycoprotein encoded by env gene of human T-cell leukemia virus. Gann. 1983 Dec;74(6):790–793. [PubMed]
  • Kettmann R, Deschamps J, Cleuter Y, Couez D, Burny A, Marbaix G. Leukemogenesis by bovine leukemia virus: proviral DNA integration and lack of RNA expression of viral long terminal repeat and 3' proximate cellular sequences. Proc Natl Acad Sci U S A. 1982 Apr;79(8):2465–2469. [PMC free article] [PubMed]
  • Kettmann R, Deschamps J, Couez D, Claustriaux JJ, Palm R, Burny A. Chromosome integration domain for bovine leukemia provirus in tumors. J Virol. 1983 Jul;47(1):146–150. [PMC free article] [PubMed]
  • Lee TH, Coligan JE, Homma T, McLane MF, Tachibana N, Essex M. Human T-cell leukemia virus-associated membrane antigens: identity of the major antigens recognized after virus infection. Proc Natl Acad Sci U S A. 1984 Jun;81(12):3856–3860. [PMC free article] [PubMed]
  • Lenz J, Crowther R, Straceski A, Haseltine W. Nucleotide sequence of the Akv env gene. J Virol. 1982 May;42(2):519–529. [PMC free article] [PubMed]
  • Maxam AM, Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. [PubMed]
  • Miyoshi I, Taguchi H, Fujishita M, Yoshimoto S, Kubonishi I, Ohtsuki Y, Shiraishi Y, Akagi T. Transformation of monkey lymphocytes with adult T-cell leukaemia virus. Lancet. 1982 May 1;1(8279):1016–1016. [PubMed]
  • Needleman SB, Wunsch CD. A general method applicable to the search for similarities in the amino acid sequence of two proteins. J Mol Biol. 1970 Mar;48(3):443–453. [PubMed]
  • Onuma M, Watarai S, Suneya M, Mikami T, Izawa H. Induction of transformed phenotypes in sheep fibroblasts by culture fluids from cells persistently infected with bovine leukemia virus. Microbiol Immunol. 1981;25(5):445–454. [PubMed]
  • Oroszlan S, Sarngadharan MG, Copeland TD, Kalyanaraman VS, Gilden RV, Gallo RC. Primary structure analysis of the major internal protein p24 of human type C T-cell leukemia virus. Proc Natl Acad Sci U S A. 1982 Feb;79(4):1291–1294. [PMC free article] [PubMed]
  • Poiesz BJ, Ruscetti FW, Gazdar AF, Bunn PA, Minna JD, Gallo RC. Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7415–7419. [PMC free article] [PubMed]
  • Rhim JS, Kraus M, Arnstein P. Neoplastic transformation of fetal lamb kidney cells by bovine leukemia virus. Int J Cancer. 1983 Jun 15;31(6):791–795. [PubMed]
  • Sagata N, Ogawa Y, Kawamura J, Onuma M, Izawa H, Ikawa Y. Molecular cloning of bovine leukemia virus DNA integrated into the bovine tumor cell genome. Gene. 1983 Dec;26(1):1–10. [PubMed]
  • Sagata N, Yasunaga T, Ogawa Y, Tsuzuku-Kawamura J, Ikawa Y. Bovine leukemia virus: unique structural features of its long terminal repeats and its evolutionary relationship to human T-cell leukemia virus. Proc Natl Acad Sci U S A. 1984 Aug;81(15):4741–4745. [PMC free article] [PubMed]
  • Seiki M, Hattori S, Hirayama Y, Yoshida M. Human adult T-cell leukemia virus: complete nucleotide sequence of the provirus genome integrated in leukemia cell DNA. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3618–3622. [PMC free article] [PubMed]
  • Seiki M, Eddy R, Shows TB, Yoshida M. Nonspecific integration of the HTLV provirus genome into adult T-cell leukaemia cells. Nature. 1984 Jun 14;309(5969):640–642. [PubMed]
  • Toh H, Hayashida H, Miyata T. Sequence homology between retroviral reverse transcriptase and putative polymerases of hepatitis B virus and cauliflower mosaic virus. Nature. 305(5937):827–829. [PubMed]
  • Yamamoto N, Okada M, Koyanagi Y, Kannagi M, Hinuma Y. Transformation of human leukocytes by cocultivation with an adult T cell leukemia virus producer cell line. Science. 1982 Aug 20;217(4561):737–739. [PubMed]
  • Yoshida M. Human leukemia virus associated with adult T-cell leukemia. Gann. 1983 Dec;74(6):777–789. [PubMed]
  • Yoshida M, Miyoshi I, Hinuma Y. Isolation and characterization of retrovirus from cell lines of human adult T-cell leukemia and its implication in the disease. Proc Natl Acad Sci U S A. 1982 Mar;79(6):2031–2035. [PMC free article] [PubMed]

Articles from The EMBO Journal are provided here courtesy of The European Molecular Biology Organization

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...