• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of embojLink to Publisher's site
EMBO J. Nov 1984; 3(11): 2543–2548.
PMCID: PMC557726

Characterization and developmental expression of beta tubulin genes in Drosophila melanogaster.

Abstract

Genomic clones containing beta tubulin sequences were isolated from a lambda library of Drosophila melanogaster. In situ hybridization localized three genes to 56D and 60B on chromosome 2 as well as to 85D on chromosome 3. The latter was known through genetic analysis to be specifically expressed during spermatogenesis. The genomic clone, pTu85, derived from this region contains one complete beta tubulin coding region as well as the 3' end of an additional so far unidentified beta tubulin gene. Genomic Southern hybridizations reveal a total of five fragments with beta tubulin homology. Clone pTu56 codes for an RNA of 1.8 kb which is expressed in all developmental stages. Clone pTu60 codes for a 2.5-kb RNA expressed during embryogenesis and pupation. In testes RNA we detected a 2.2-kb message homologous to pTu85.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.6M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Benton WD, Davis RW. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. [PubMed]
  • Brunke KJ, Young EE, Buchbinder BU, Weeks DP. Coordinate regulation of the four tubulin genes of Chlamydomonas reinhardi. Nucleic Acids Res. 1982 Feb 25;10(4):1295–1310. [PMC free article] [PubMed]
  • Bünemann H. Immobilization of denatured DNA to macroporous supports: II. Steric and kinetic parameters of heterogeneous hybridization reactions. Nucleic Acids Res. 1982 Nov 25;10(22):7181–7196. [PMC free article] [PubMed]
  • Bünemann H, Westhoff P, Herrmann RG. Immobilization of denatured DNA to macroporous supports: I. Efficiency of different coupling procedures. Nucleic Acids Res. 1982 Nov 25;10(22):7163–7180. [PMC free article] [PubMed]
  • Cleveland DW. The tubulins: from DNA to RNA to protein and back again. Cell. 1983 Sep;34(2):330–332. [PubMed]
  • Cleveland DW, Lopata MA, MacDonald RJ, Cowan NJ, Rutter WJ, Kirschner MW. Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes. Cell. 1980 May;20(1):95–105. [PubMed]
  • Cowan NJ, Dudley L. Tubulin isotypes and the multigene tubulin families. Int Rev Cytol. 1983;85:147–173. [PubMed]
  • Goldberg DA. Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene. Proc Natl Acad Sci U S A. 1980 Oct;77(10):5794–5798. [PMC free article] [PubMed]
  • Kalfayan L, Wensink PC. alpha-Tubulin genes of Drosophila. Cell. 1981 Apr;24(1):97–106. [PubMed]
  • Kalfayan L, Wensink PC. Developmental regulation of Drosophila alpha-tubulin genes. Cell. 1982 May;29(1):91–98. [PubMed]
  • Kemphues KJ, Raff RA, Kaufman TC, Raff EC. Mutation in a structural gene for a beta-tubulin specific to testis in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3991–3995. [PMC free article] [PubMed]
  • Kemphues KJ, Raff EC, Raff RA, Kaufman TC. Mutation in a testis-specific beta-tubulin in Drosophila: analysis of its effects on meiosis and map location of the gene. Cell. 1980 Sep;21(2):445–451. [PubMed]
  • Kemphues KJ, Kaufman TC, Raff RA, Raff EC. The testis-specific beta-tubulin subunit in Drosophila melanogaster has multiple functions in spermatogenesis. Cell. 1982 Dec;31(3 Pt 2):655–670. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • LeMeur M, Glanville N, Mandel JL, Gerlinger P, Palmiter R, Chambon P. The ovalbumin gene family: hormonal control of X and Y gene transcription and mRNA accumulation. Cell. 1981 Feb;23(2):561–571. [PubMed]
  • Maxam AM, Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. [PubMed]
  • Mischke D, Pardue ML. Organization and expression of alpha-tubulin genes in Drosophila melanogaster. One member of the alpha-tubulin multigene family is transcribed in both oogenesis and later embryonic development. J Mol Biol. 1982 Apr 15;156(3):449–466. [PubMed]
  • Neff NF, Thomas JH, Grisafi P, Botstein D. Isolation of the beta-tubulin gene from yeast and demonstration of its essential function in vivo. Cell. 1983 May;33(1):211–219. [PubMed]
  • O'Farrell PH. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed]
  • Raff EC, Fuller MT, Kaufman TC, Kemphues KJ, Rudolph JE, Raff RA. Regulation of tubulin gene expression during embryogenesis in Drosophila melanogaster. Cell. 1982 Jan;28(1):33–40. [PubMed]
  • Renkawitz-Pohl R, Bialojan S. A DNA sequence of Drosophila melanogaster with a differential telomeric distribution. Chromosoma. 1984;89(3):206–211. [PubMed]
  • Renkawitz-Pohl R, Glätzer KH, Kunz W. Characterization of cloned ribosomal DNA from Drosophila hydei. Nucleic Acids Res. 1980 Oct 24;8(20):4593–4611. [PMC free article] [PubMed]
  • Sánchez F, Natzle JE, Cleveland DW, Kirschner MW, McCarthy BJ. A dispersed multigene family encoding tubulin in Drosophila melanogaster. Cell. 1980 Dec;22(3):845–854. [PubMed]
  • Silflow CD, Rosenbaum JL. Multiple alpha- and beta-tubulin genes in Chlamydomonas and regulation of tubulin mRNA levels after deflagellation. Cell. 1981 Apr;24(1):81–88. [PubMed]
  • Ullrich A, Shine J, Chirgwin J, Pictet R, Tischer E, Rutter WJ, Goodman HM. Rat insulin genes: construction of plasmids containing the coding sequences. Science. 1977 Jun 17;196(4296):1313–1319. [PubMed]

Articles from The EMBO Journal are provided here courtesy of The European Molecular Biology Organization

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

    Your browsing activity is empty.

    Activity recording is turned off.

    Turn recording back on

    See more...