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EMBO J. May 1984; 3(5): 1151–1157.
PMCID: PMC557488

A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer.


DNA of a new papillomavirus type was cloned from a cervical carcinoma biopsy. Two EcoRI clones of 7.8 and 6.9 kb in length were obtained, the latter contained a 900-bp deletion. The BamHI fragments of both clones were used to characterize the DNA. It represents a distinct type of papillomavirus as determined by its size, its cross-hybridization with DNA of other papillomavirus types under conditions of low stringency only, the co-linear alignment of its genome with HPV 6 and HPV 16 prototypes and its occasional occurrence as oligomeric episomes. We tentatively propose to designate it as HPV 18. DNA hybridizing with HPV 18 under stringent conditions was detected in 9/36 cervical carcinomas from Africa and Brazil, in 2/13 cervical tumors from Germany and 1/10 penile carcinomas. Benign tumors (17 cervical dysplasias, 29 genital warts), eight carcinomata in situ and 15 biopsies of normal cervical tissue were devoid of detectable HPV 18 DNA. HPV 18-related DNA was found, however, in cells of the HeLa, KB and C4-1 lines all derived from cervical cancer. The state of the viral DNA was investigated in four cervical cancer biopsies. The data reveal that the DNA might be integrated into the host cell genome. One tumor provided evidence for head to tail tandem repeats some of which persisted as circular episomes.

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Selected References

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  • Breitburd F, Favre M, Zoorob R, Fortin D, Orth G. Detection and characterization of viral genomes and search for tumoral antigens in two hamster cell lines derived from tumors induced by bovine papillomavirus type 1. Int J Cancer. 1981 May 15;27(5):693–702. [PubMed]
  • de Villiers EM, Gissmann L, zur Hausen H. Molecular cloning of viral DNA from human genital warts. J Virol. 1981 Dec;40(3):932–935. [PMC free article] [PubMed]
  • Dürst M, Gissmann L, Ikenberg H, zur Hausen H. A papillomavirus DNA from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3812–3815. [PMC free article] [PubMed]
  • Fresen KO, Cho MS, Gissmann L, zur Hausen H. NC37-R1 Epstein-Barr virus (EBV): a possible recombinant between intracellular NC37 viral DNA and superinfecting P3HR-1 EBV. Intervirology. 1980;12(6):303–310. [PubMed]
  • Gissmann L, deVilliers EM, zur Hausen H. Analysis of human genital warts (condylomata acuminata) and other genital tumors for human papillomavirus type 6 DNA. Int J Cancer. 1982 Feb 15;29(2):143–146. [PubMed]
  • Gissmann L, Diehl V, Schultz-Coulon HJ, zur Hausen H. Molecular cloning and characterization of human papilloma virus DNA derived from a laryngeal papilloma. J Virol. 1982 Oct;44(1):393–400. [PMC free article] [PubMed]
  • Gissmann L, Wolnik L, Ikenberg H, Koldovsky U, Schnürch HG, zur Hausen H. Human papillomavirus types 6 and 11 DNA sequences in genital and laryngeal papillomas and in some cervical cancers. Proc Natl Acad Sci U S A. 1983 Jan;80(2):560–563. [PMC free article] [PubMed]
  • Green M, Brackmann KH, Sanders PR, Loewenstein PM, Freel JH, Eisinger M, Switlyk SA. Isolation of a human papillomavirus from a patient with epidermodysplasia verruciformis: presence of related viral DNA genomes in human urogenital tumors. Proc Natl Acad Sci U S A. 1982 Jul;79(14):4437–4441. [PMC free article] [PubMed]
  • Ikenberg H, Gissmann L, Gross G, Grussendorf-Conen EI, zur Hausen H. Human papillomavirus type-16-related DNA in genital Bowen's disease and in Bowenoid papulosis. Int J Cancer. 1983 Nov 15;32(5):563–565. [PubMed]
  • McVay P, Fretz M, Wettstein F, Stevens J, Ito Y. Integrated shope virus DNA is present and transcribed in the transplantable rabbit tumour Vx-7. J Gen Virol. 1982 Jun;60(Pt 2):271–278. [PubMed]
  • Nelson-Rees WA, Flandermeyer RR. HeLa cultures defined. Science. 1976 Jan 9;191(4222):96–98. [PubMed]
  • Orth G, Jablonska S, Favre M, Croissant O, Obalek S, Jarzabek-Chorzelska M, Jibard N. Identification of papillomaviruses in butchers' warts. J Invest Dermatol. 1981 Feb;76(2):97–102. [PubMed]
  • Ostrow RS, Bender M, Niimura M, Seki T, Kawashima M, Pass F, Faras AJ. Human papillomavirus DNA in cutaneous primary and metastasized squamous cell carcinomas from patients with epidermodysplasia verruciformis. Proc Natl Acad Sci U S A. 1982 Mar;79(5):1634–1638. [PMC free article] [PubMed]
  • Pfister H. Biology and biochemistry of papillomaviruses. Rev Physiol Biochem Pharmacol. 1984;99:111–181. [PubMed]
  • Pfister H, Gassenmaier A, Nürnberger F, Stüttgen G. Human papilloma virus 5-DNA in a carcinoma of an epidermodysplasia verruciformis patient infected with various human papillomavirus types. Cancer Res. 1983 Mar;43(3):1436–1441. [PubMed]
  • Rigby PW, Dieckmann M, Rhodes C, Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. [PubMed]
  • Rowson KE, Mahy BW. Human papova (wart) virus. Bacteriol Rev. 1967 Jun;31(2):110–131. [PMC free article] [PubMed]
  • Schwarz E, Dürst M, Demankowski C, Lattermann O, Zech R, Wolfsperger E, Suhai S, zur Hausen H. DNA sequence and genome organization of genital human papillomavirus type 6b. EMBO J. 1983;2(12):2341–2348. [PMC free article] [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Wettstein FO, Stevens JG. Variable-sized free episomes of Shope papilloma virus DNA are present in all non-virus-producing neoplasms and integrated episomes are detected in some. Proc Natl Acad Sci U S A. 1982 Feb;79(3):790–794. [PMC free article] [PubMed]
  • zur Hausen H. Human papillomaviruses and their possible role in squamous cell carcinomas. Curr Top Microbiol Immunol. 1977;78:1–30. [PubMed]
  • zur Hausen H, Schulte-Holthausen H, Klein G, Henle W, Henle G, Clifford P, Santesson L. EBV DNA in biopsies of Burkitt tumours and anaplastic carcinomas of the nasopharynx. Nature. 1970 Dec 12;228(5276):1056–1058. [PubMed]

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