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EMBO J. Dec 30, 1985; 4(13B): 3867–3872.
PMCID: PMC554742

DNA intercalators induce specific release of HMG 14, HMG 17 and other DNA-binding proteins from chicken erythrocyte chromatin.

Abstract

Chicken erythrocyte nuclei were incubated with DNA intercalating agents in order to isolate from chromatin specific DNA-binding proteins whose binding specificity may be determined by DNA secondary and/or tertiary structure. The intercalating agents ethidium bromide (EtBr) and propidium iodide induce the specific release of high mobility group proteins HMG 14 and HMG 17 under low ionic strength conditions. Chloroquine (CQ) intercalation also results in the selective liberation of HMG 14 and HMG 17, but, in addition, selectively releases other nuclear proteins (including histone H1A) in a pH- and ionic strength-dependent fashion. The use of this new 'elutive intercalation' technique for the isolation and purification of 'sequence-specific' and 'helix-specific' DNA-binding proteins is suggested.

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  • Frederick CA, Grable J, Melia M, Samudzi C, Jen-Jacobson L, Wang BC, Greene P, Boyer HW, Rosenberg JM. Kinked DNA in crystalline complex with EcoRI endonuclease. Nature. 1984 May 24;309(5966):327–331. [PubMed]
  • Hunkapiller MW, Lujan E, Ostrander F, Hood LE. Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis. Methods Enzymol. 1983;91:227–236. [PubMed]
  • Hunkapiller MW, Hewick RM, Dreyer WJ, Hood LE. High-sensitivity sequencing with a gas-phase sequenator. Methods Enzymol. 1983;91:399–413. [PubMed]
  • Igo-Kemenes T, Hörz W, Zachau HG. Chromatin. Annu Rev Biochem. 1982;51:89–121. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Levy W B, Wong NC, Dixon GH. Selective association of the trout-specific H6 protein with chromatin regions susceptible to DNase I and DNase II: possible location of HMG-T in the spacer region between core nucleosomes. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2810–2814. [PMC free article] [PubMed]
  • Levy-Wilson B, Kuehl L, Dixon GH. The release of high mobility group protein H6 and protamine gene sequences upon selective DNase I degradation of trout testis chromatin. Nucleic Acids Res. 1980 Jul 11;8(13):2859–2869. [PMC free article] [PubMed]
  • Lottspeich F. Identification of the phenylthiohydantoin derivatives of amino acids by high pressure liquid chromatography, using a ternary, isocratic solvent system. Hoppe Seylers Z Physiol Chem. 1980 Dec;361(12):1829–1834. [PubMed]
  • Nickol JM, Felsenfeld G. DNA conformation at the 5' end of the chicken adult beta-globin gene. Cell. 1983 Dec;35(2 Pt 1):467–477. [PubMed]
  • Nordheim A, Tesser P, Azorin F, Kwon YH, Möller A, Rich A. Isolation of Drosophila proteins that bind selectively to left-handed Z-DNA. Proc Natl Acad Sci U S A. 1982 Dec;79(24):7729–7733. [PMC free article] [PubMed]
  • Pabo CO, Sauer RT. Protein-DNA recognition. Annu Rev Biochem. 1984;53:293–321. [PubMed]
  • Paton AE, Wilkinson-Singley E, Olins DE. Nonhistone nuclear high mobility group proteins 14 and 17 stabilize nucleosome core particles. J Biol Chem. 1983 Nov 10;258(21):13221–13229. [PubMed]
  • Pohl FM, Jovin TM, Baehr W, Holbrook JJ. Ethidium bromide as a cooperative effector of a DNA structure. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3805–3809. [PMC free article] [PubMed]
  • Richmond TJ, Finch JT, Rushton B, Rhodes D, Klug A. Structure of the nucleosome core particle at 7 A resolution. Nature. 1984 Oct 11;311(5986):532–537. [PubMed]
  • Sandeen G, Wood WI, Felsenfeld G. The interaction of high mobility proteins HMG14 and 17 with nucleosomes. Nucleic Acids Res. 1980 Sep 11;8(17):3757–3778. [PMC free article] [PubMed]
  • Schröter H, Bode J. The binding sites for large and small high-mobility-group (HMG) proteins. Studies on HMG-nucleosome interactions in vitro. Eur J Biochem. 1982 Oct;127(2):429–436. [PubMed]
  • Spiker S, Mardian JK, Isenberg I. Chomosomal HMG proteins occur in three eukaryotic kingdoms. Biochem Biophys Res Commun. 1978 May 15;82(1):129–135. [PubMed]
  • Strätling WH, Seidel I. Relaxation of chromatin structure by ethidium bromide binding: determined by viscometry and histone dissociation studies. Biochemistry. 1976 Nov 2;15(22):4803–4809. [PubMed]
  • Walker JM, Johns EW. The isolation, characterization and partial sequences of the chicken erythrocyte non-histone chromosomal proteins HMG14 and HMG17. Comparison with the homologous calf thymus proteins. Biochem J. 1980 Feb 1;185(2):383–386. [PMC free article] [PubMed]
  • Walker JM, Stearn C, Johns EW. The primary structure of non-histone chromosomal protein HMG17 from chicken erythrocyte nuclei. FEBS Lett. 1980 Apr 7;112(2):207–210. [PubMed]
  • Wang JC, Peck LJ, Becherer K. DNA supercoiling and its effects on DNA structure and function. Cold Spring Harb Symp Quant Biol. 1983;47(Pt 1):85–91. [PubMed]
  • Watson DC, Wong NC, Dixon GH. The complete amino-acid sequence of a trout-testis non-histone protein, H6, localized in a subset of nucleosomes and its similarity to calf-thymus non-histone proteins HMG-14 and HMG-17. Eur J Biochem. 1979 Mar 15;95(1):193–202. [PubMed]
  • Weisbrod S. Active chromatin. Nature. 1982 May 27;297(5864):289–295. [PubMed]
  • Weisbrod S, Weintraub H. Isolation of a subclass of nuclear proteins responsible for conferring a DNase I-sensitive structure on globin chromatin. Proc Natl Acad Sci U S A. 1979 Feb;76(2):630–634. [PMC free article] [PubMed]
  • Yanisch-Perron C, Vieira J, Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. [PubMed]
  • Zimmerman SB. The three-dimensional structure of DNA. Annu Rev Biochem. 1982;51:395–427. [PubMed]

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