• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of embojLink to Publisher's site
EMBO J. Dec 30, 1985; 4(13B): 3797–3803.
PMCID: PMC554733

Developmentally regulated transcription of Dictyostelium discoideum plasmid Ddp1


The presence of high copy number plasmid DNA has been described for several independent wild isolates of the lower eukaryote Dictyostelium discoideum, a model organism in developmental biology. The function of these plasmids has remained unclear so far. To understand the possible functions of the plasmids we have analyzed the transcription of one plasmid, Ddp1, in the wild strains NC4 and V12. The plasmid is transcribed in an identical fashion in both strains. Ddp1 codes for three RNA species in growth-phase cells, at least two of which are present in the poly(A)+ fraction. RNA isolated from cells of different developmental stages revealed the existence of five more transcripts, the majority of them being found in the poly(A)+ fraction. These transcripts are present at distinct time points during development. Both growth-phase and development-specific transcripts were found exclusively in Ddp1-containing strains. Mapping of the transcripts to the plasmid showed that some of them originated from the same area. These results indicated an overlapping of their coding regions.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.5M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Alton TH, Lodish HF. Developmental changes in messenger RNAs and protein synthesis in Dictyostelium discoideum. Dev Biol. 1977 Oct 1;60(1):180–206. [PubMed]
  • Birnboim HC, Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. [PMC free article] [PubMed]
  • Chang AC, Cohen SN. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. [PMC free article] [PubMed]
  • Chisholm RL, Barklis E, Lodish HF. Mechanism of sequential induction of cell-type specific mRNAs in Dictyostelium differentiation. Nature. 1984 Jul 5;310(5972):67–69. [PubMed]
  • Dretzen G, Bellard M, Sassone-Corsi P, Chambon P. A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. Anal Biochem. 1981 Apr;112(2):295–298. [PubMed]
  • Engel JD, Sugarman BJ, Dodgson JB. A chicken histone H3 gene contains intervening sequences. Nature. 1982 Jun 3;297(5865):434–436. [PubMed]
  • Firtel RA, Lodish HF. A small nuclear precursor of messenger RNA in the cellular slime mold Dictyostelium discoideum. J Mol Biol. 1973 Sep 15;79(2):295–314. [PubMed]
  • Goldberg DA. Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene. Proc Natl Acad Sci U S A. 1980 Oct;77(10):5794–5798. [PMC free article] [PubMed]
  • Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. [PubMed]
  • Holmes DS, Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981 Jun;114(1):193–197. [PubMed]
  • Krefft M, Voet L, Gregg JH, Mairhofer H, Williams KL. Evidence that positional information is used to establish the prestalk-prespore pattern in Dictyostelium discoideum aggregates. EMBO J. 1984 Jan;3(1):201–206. [PMC free article] [PubMed]
  • Landfear SM, Lefebvre P, Chung S, Lodish HF. Transcriptional control of gene expression during development of Dictyostelium discoideum. Mol Cell Biol. 1982 Nov;2(11):1417–1426. [PMC free article] [PubMed]
  • Mehdy MC, Ratner D, Firtel RA. Induction and modulation of cell-type-specific gene expression in Dictyostelium. Cell. 1983 Mar;32(3):763–771. [PubMed]
  • Metz BA, Ward TE, Welker DL, Williams KL. Identification of an endogenous plasmid in Dictyostelium discoideum. EMBO J. 1983;2(4):515–519. [PMC free article] [PubMed]
  • Noegel A, Welker DL, Metz BA, Williams KL. Presence of nuclear associated plasmids in the lower eukaryote Dictyostelium discoideum. J Mol Biol. 1985 Sep 20;185(2):447–450. [PubMed]
  • Palatnik CM, Storti RV, Jacobson A. Fractionation and functional analysis of newly synthesized and decaying messenger RNAs from vegetative cells of Dictyostelium discoideum. J Mol Biol. 1979 Mar 5;128(3):371–395. [PubMed]
  • Palatnik CM, Wilkins C, Jacobson A. Translational control during early Dictyostelium development: possible involvement of poly(A) sequences. Cell. 1984 Apr;36(4):1017–1025. [PubMed]
  • Rigby PW, Dieckmann M, Rhodes C, Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Watts DJ, Ashworth JM. Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture. Biochem J. 1970 Sep;119(2):171–174. [PMC free article] [PubMed]
  • Williams JG, Lloyd MM. Changes in the abundance of polyadenylated RNA during slime mould development measured using cloned molecular hybridization probes. J Mol Biol. 1979 Mar 25;129(1):19–35. [PubMed]

Articles from The EMBO Journal are provided here courtesy of The European Molecular Biology Organization


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...