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EMBO J. Dec 16, 1985; 4(13A): 3553–3556.
PMCID: PMC554696

Cloning of opal suppressor tRNA genes of a filamentous fungus reveals two tRNASerUGA genes with unexpected structural differences.


The informational suppressors su4-1 and su8-1 of Podospora anserina were isolated by transformation of Schizosaccharomyces pombe UGA mutants. The DNA sequence revealed that they were opal (UGA) suppressor tRNAs. Wild-type alleles were also isolated by hybridization. The DNA sequence showed that they both encode species of tRNASerUGA. The gene SU8 has an 18-bp intervening sequence and its primary sequence is very different from that of SU4.

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  • Amstutz H, Munz P, Heyer WD, Leupoid U, Kohli J. Concerted evolution of tRNA genes: intergenic conversion among three unlinked serine tRNA genes in S. pombe. Cell. 1985 Apr;40(4):879–886. [PubMed]
  • Beach D, Piper M, Nurse P. Construction of a Schizosaccharomyces pombe gene bank in a yeast bacterial shuttle vector and its use to isolate genes by complementation. Mol Gen Genet. 1982;187(2):326–329. [PubMed]
  • Broach JR, Friedman L, Sherman F. Correspondence of yeast UAA suppressors to cloned tRNASerUCA genes. J Mol Biol. 1981 Aug 15;150(3):375–387. [PubMed]
  • Cedergren RJ, Sankoff D, LaRue B, Grosjean H. The evolving tRNA molecule. CRC Crit Rev Biochem. 1981;11(1):35–104. [PubMed]
  • Crick FH. Codon--anticodon pairing: the wobble hypothesis. J Mol Biol. 1966 Aug;19(2):548–555. [PubMed]
  • Gamulin V, Mao J, Appel B, Sumner-Smith M, Yamao F, Söll D. Six Schizosaccharomyces pombe tRNA genes including a gene for a tRNALys with an intervening sequence which cannot base-pair with the anticodon. Nucleic Acids Res. 1983 Dec 20;11(24):8537–8546. [PMC free article] [PubMed]
  • Grosjean H, Cedergren RJ, McKay W. Structure in tRNA data. Biochimie. 1982 Jun;64(6):387–397. [PubMed]
  • Gupta RC, Roe BA, Randerath K. Sequence of human glycine transfer ribonucleic acid (anticodon CCC). Determination by a newly developed thin-layer readout sequencing technique and comparison with other glycine transfer ribonucleic acids. Biochemistry. 1980 Apr 15;19(8):1699–1705. [PubMed]
  • Johnson PF, Abelson J. The yeast tRNATyr gene intron is essential for correct modification of its tRNA product. Nature. 1983 Apr 21;302(5910):681–687. [PubMed]
  • Klein HL, Petes TD. Intrachromosomal gene conversion in yeast. Nature. 1981 Jan 15;289(5794):144–148. [PubMed]
  • Kobayashi T, Irie T, Yoshida M, Takeishi K, Ukita T. The primary structure of yeast glutamic acid tRNA specific to the GAA codon. Biochim Biophys Acta. 1974 Oct 11;366(2):168–181. [PubMed]
  • Munz P, Amstutz H, Kohli J, Leupold U. Recombination between dispersed serine tRNA genes in Schizosaccharomyces pombe. Nature. 1982 Nov 18;300(5889):225–231. [PubMed]
  • Munz P, Leupold U, Agris P, Kohli J. In vivo decoding rules in Schizosaccharomyces pombe are at variance with in vitro data. Nature. 1981 Nov 12;294(5837):187–188. [PubMed]
  • Page GS, Hall BD. Characterization of the yeast tRNA Ser genomic organization and DNA sequence. Nucleic Acids Res. 1981 Feb 25;9(4):921–934. [PMC free article] [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Sekiya T, Takeishi K, Ukita T. Specificity of yeast glutamic acid transfer RNA for codon recognition. Biochim Biophys Acta. 1969 Jun 17;182(2):411–426. [PubMed]
  • Shortridge RD, Pirtle IL, Pirtle RM. Nucleotide sequence and transcription of a gene encoding human tRNAGlyCCC. Gene. 1985;33(3):269–277. [PubMed]
  • Sprinzl M, Gauss DH. Compilation of tRNA sequences. Nucleic Acids Res. 1984;12 (Suppl):r1–57. [PMC free article] [PubMed]
  • Sumner-Smith M, Hottinger H, Willis I, Koch TL, Arentzen R, Söll D. The sup8 tRNALeu gene of Schizosaccharomyces pombe has an unusual intervening sequence and reduced pairing in the anticodon stem. Mol Gen Genet. 1984;197(3):447–452. [PubMed]
  • Traboni C, Ciliberto G, Cortese R. A novel method for site-directed mutagenesis: its application to an eukaryotic tRNAPro gene promoter. EMBO J. 1982;1(4):415–420. [PMC free article] [PubMed]

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