• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of embojLink to Publisher's site
EMBO J. Mar 1985; 4(3): 781–785.
PMCID: PMC554256

Molecular cloning of Erwinia chrysanthemi pectinase and cellulase structural genes


Erwinia chrysanthemi 3937 secretes four major pectate lyase isoenzymes (PL, EC and one endocellulase (Cx, EC A genomic library of this strain was constructed in the Lambda L47-1 vector, and screened for the presence of PL and Cx on pectate and caboxymethylcellulose agar. Among the seven Cx-positive phage clones, three were shown to encode an enzyme of the same mol. wt. as the one found in the culture supernatant of strain 3937. The 34 PL-positive phage clones were analyzed by electrofocusing and could, according to the PL they produced, be arranged in five classes. Phages from three classes produced three different single PL, named PLb, c and d. No common fragment was evidenced between the inserts of the phages of these three classes. This demonstrated that, in strain 3937, PLb, C, and d were encoded by three different genes called pelB, C, and D. Furthermore, our results suggest the existence of two additional genes encoding PLa and e. In addition, a pectin methylesterase gene was found closely linked to pelD.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Andro T, Chambost JP, Kotoujansky A, Cattaneo J, Bertheau Y, Barras F, Van Gijsegem F, Coleno A. Mutants of Erwinia chrysanthemi defective in secretion of pectinase and cellulase. J Bacteriol. 1984 Dec;160(3):1199–1203. [PMC free article] [PubMed]
  • Bertheau Y, Madgidi-Hervan E, Kotoujansky A, Nguyen-The C, Andro T, Coleno A. Detection of depolymerase isoenzymes after electrophoresis or electrofocusing, or in titration curves. Anal Biochem. 1984 Jun;139(2):383–389. [PubMed]
  • Chatterjee AK, Brown MA. Generalized transduction in the enterobacterial phytopathogen Erwinia chrysanthemi. J Bacteriol. 1980 Sep;143(3):1444–1449. [PMC free article] [PubMed]
  • Chatterjee AK, Starr MP. Donor strains of the soft-rot bacterium Erwinia chrysanthemi and conjugational transfer of the pectolytic capacity. J Bacteriol. 1977 Dec;132(3):862–869. [PMC free article] [PubMed]
  • Clarke L, Carbon J. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell. 1976 Sep;9(1):91–99. [PubMed]
  • Cruickshank RH, Wade GC. Detection of pectic enzymes in pectin-acrylamide gels. Anal Biochem. 1980 Sep 1;107(1):177–181. [PubMed]
  • Enquist L, Sternberg N. In vitro packaging of lambda Dam vectors and their use in cloning DNA fragments. Methods Enzymol. 1979;68:281–298. [PubMed]
  • Hohn B. In vitro packaging of lambda and cosmid DNA. Methods Enzymol. 1979;68:299–309. [PubMed]
  • Kotoujansky A, Lemattre M, Boistard P. Utilization of a thermosensitive episome bearing transposon TN10 to isolate Hfr donor strains of Erwinia carotovora subsp. chrysanthemi. J Bacteriol. 1982 Apr;150(1):122–131. [PMC free article] [PubMed]
  • Loenen WA, Brammar WJ. A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes. Gene. 1980 Aug;10(3):249–259. [PubMed]
  • PREISS J, ASHWELL G. Polygalacturonic acid metabolism in bacteria. I. Enzymatic formation of 4-deoxy-L-threo-5-hexoseulose uronic acid. J Biol Chem. 1963 May;238:1571–1583. [PubMed]
  • Schoonejans E, Toussaint A. Utilization of plasmid pULB113 (RP4::mini-Mu) to construct a linkage map of Erwinia carotovora subsp. chrysanthemi. J Bacteriol. 1983 Jun;154(3):1489–1492. [PMC free article] [PubMed]
  • Starr MP, Chatterjee AK. The genus Erwinia: enterobacteria pathogenic to plants and animals. Annu Rev Microbiol. 1972;26:389–426. [PubMed]

Articles from The EMBO Journal are provided here courtesy of The European Molecular Biology Organization


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...