• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of embojLink to Publisher's site
EMBO J. May 1987; 6(5): 1459–1465.
PMCID: PMC553952

The receptor-binding and membrane-fusion properties of influenza virus variants selected using anti-haemagglutinin monoclonal antibodies.

Abstract

A monoclonal antibody raised against X-31 influenza virus reacted with the majority of natural H3N2 viruses isolated between 1968 and 1982. A number of variants of X-31 and of a receptor-binding mutant of X-31 were selected by the antibody during virus replication in eggs and MDCK cells. Antibody-binding assays indicated that the viruses selected were not antigenic variants and analyses using derivatized erythrocytes showed that their receptor-binding properties differed from those of the parent viruses. The amino acid substitutions in the variants were all located in the vicinity of the receptor-binding site and the structural consequences are discussed in relation to the three-dimensional structure of X-31 HA. In addition all of the variants fused membranes at higher pH than wild-type virus indicating that structural modifications in the distal globular region of HA influence the low pH-induced conformational change required for membrane fusion.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Appleyard G, Maber HB. Plaque formation by influenza viruses in the presence of trypsin. J Gen Virol. 1974 Dec;25(3):351–357. [PubMed]
  • Bos ES, van der Doelen AA, van Rooy N, Schuurs AH. 3,3',5,5' - Tetramethylbenzidine as an Ames test negative chromogen for horse-radish peroxidase in enzyme-immunoassay. J Immunoassay. 1981;2(3-4):187–204. [PubMed]
  • Brand CM, Skehel JJ. Crystalline antigen from the influenza virus envelope. Nat New Biol. 1972 Aug 2;238(83):145–147. [PubMed]
  • Carroll SM, Higa HH, Paulson JC. Different cell-surface receptor determinants of antigenically similar influenza virus hemagglutinins. J Biol Chem. 1981 Aug 25;256(16):8357–8363. [PubMed]
  • Caton AJ, Brownlee GG, Yewdell JW, Gerhard W. The antigenic structure of the influenza virus A/PR/8/34 hemagglutinin (H1 subtype). Cell. 1982 Dec;31(2 Pt 1):417–427. [PubMed]
  • Daniels RS, Douglas AR, Skehel JJ, Wiley DC. Analyses of the antigenicity of influenza haemagglutinin at the pH optimum for virus-mediated membrane fusion. J Gen Virol. 1983 Aug;64(Pt 8):1657–1662. [PubMed]
  • Daniels RS, Douglas AR, Skehel JJ, Wiley DC, Naeve CW, Webster RG, Rogers GN, Paulson JC. Antigenic analyses of influenza virus haemagglutinins with different receptor-binding specificities. Virology. 1984 Oct 15;138(1):174–177. [PubMed]
  • Daniels RS, Downie JC, Hay AJ, Knossow M, Skehel JJ, Wang ML, Wiley DC. Fusion mutants of the influenza virus hemagglutinin glycoprotein. Cell. 1985 Feb;40(2):431–439. [PubMed]
  • Laver WG, Gerhard W, Webster RG, Frankel ME, Air GM. Antigenic drift in type A influenza virus: peptide mapping and antigenic analysis of A/PR/8/34 (HON1) variants selected with monoclonal antibodies. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1425–1429. [PMC free article] [PubMed]
  • Laver WG, Air GM, Webster RG, Gerhard W, Ward CW, Dopheide TA. The mechanism of antigenic drift in influenza virus: sequence changes in the haemagglutinin of variants selected with monoclonal hybridoma antibodies. Philos Trans R Soc Lond B Biol Sci. 1980 Feb 25;288(1029):313–326. [PubMed]
  • Rogers GN, Paulson JC. Receptor determinants of human and animal influenza virus isolates: differences in receptor specificity of the H3 hemagglutinin based on species of origin. Virology. 1983 Jun;127(2):361–373. [PubMed]
  • Rogers GN, Paulson JC, Daniels RS, Skehel JJ, Wilson IA, Wiley DC. Single amino acid substitutions in influenza haemagglutinin change receptor binding specificity. Nature. 1983 Jul 7;304(5921):76–78. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Skehel JJ, Schild GC. The polypeptide composition of influenza A viruses. Virology. 1971 May;44(2):396–408. [PubMed]
  • Skehel JJ, Stevens DJ, Daniels RS, Douglas AR, Knossow M, Wilson IA, Wiley DC. A carbohydrate side chain on hemagglutinins of Hong Kong influenza viruses inhibits recognition by a monoclonal antibody. Proc Natl Acad Sci U S A. 1984 Mar;81(6):1779–1783. [PMC free article] [PubMed]
  • Underwood PA. An antigenic map of the haemagglutinin of the influenza Hong Kong subtype (H3N2), constructed using mouse monoclonal antibodies. Mol Immunol. 1984 Jul;21(7):663–671. [PubMed]
  • Underwood PA, Skehel JJ, Wiley DC. Receptor-binding characteristics of monoclonal antibody-selected antigenic variants of influenza virus. J Virol. 1987 Jan;61(1):206–208. [PMC free article] [PubMed]
  • Webster RG, Laver WG. Determination of the number of nonoverlapping antigenic areas on Hong Kong (H3N2) influenza virus hemagglutinin with monoclonal antibodies and the selection of variants with potential epidemiological significance. Virology. 1980 Jul 15;104(1):139–148. [PubMed]
  • Wharton SA, Skehel JJ, Wiley DC. Studies of influenza haemagglutinin-mediated membrane fusion. Virology. 1986 Feb;149(1):27–35. [PubMed]
  • Wiley DC, Wilson IA, Skehel JJ. Structural identification of the antibody-binding sites of Hong Kong influenza haemagglutinin and their involvement in antigenic variation. Nature. 1981 Jan 29;289(5796):373–378. [PubMed]
  • Wilson IA, Skehel JJ, Wiley DC. Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 A resolution. Nature. 1981 Jan 29;289(5796):366–373. [PubMed]
  • Yewdell JW, Gerhard W. Antigenic characterization of viruses by monoclonal antibodies. Annu Rev Microbiol. 1981;35:185–206. [PubMed]
  • Yewdell JW, Caton AJ, Gerhard W. Selection of influenza A virus adsorptive mutants by growth in the presence of a mixture of monoclonal antihemagglutinin antibodies. J Virol. 1986 Feb;57(2):623–628. [PMC free article] [PubMed]

Articles from The EMBO Journal are provided here courtesy of The European Molecular Biology Organization

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links