Logo of embojLink to Publisher's site
EMBO J. Nov 1987; 6(11): 3449–3454.
PMCID: PMC553802

Mitochondrial protein import: involvement of the mature part of a cleavable precursor protein in the binding to receptor sites.


The precursor of F0-ATPase subunit 9 was bound to mitochondria in the absence of a mitochondrial membrane potential (delta psi). Binding was mediated by a protease-sensitive component on the mitochondrial surface. When delta psi was reestablished, bound precursor was directly imported without prior release from the mitochondrial membranes. A chimaeric protein consisting of the complete subunit 9 precursor fused to cytosolic dihydrofolate reductase (DHFR) was also specifically bound to mitochondria in the absence of delta psi. Two other fusion proteins, consisting either of the entire presequence of subunit 9 and DHFR or of part of the presequence and DHFR, were imported in the presence of delta psi. In the absence of delta psi, however, specific binding to mitochondria did not take place. We suggest that the hydrophobic mature part of subunit 9 is involved in the delta psi-independent binding of the subunit 9 precursor to receptor sites on the mitochondrial surface.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Adrian GS, McCammon MT, Montgomery DL, Douglas MG. Sequences required for delivery and localization of the ADP/ATP translocator to the mitochondrial inner membrane. Mol Cell Biol. 1986 Feb;6(2):626–634. [PMC free article] [PubMed]
  • Arends H, Sebald W. Nucleotide sequence of the cloned mRNA and gene of the ADP/ATP carrier from Neurospora crassa. EMBO J. 1984 Feb;3(2):377–382. [PMC free article] [PubMed]
  • Daum G, Gasser SM, Schatz G. Import of proteins into mitochondria. Energy-dependent, two-step processing of the intermembrane space enzyme cytochrome b2 by isolated yeast mitochondria. J Biol Chem. 1982 Nov 10;257(21):13075–13080. [PubMed]
  • Gasser SM, Ohashi A, Daum G, Böhni PC, Gibson J, Reid GA, Yonetani T, Schatz G. Imported mitochondrial proteins cytochrome b2 and cytochrome c1 are processed in two steps. Proc Natl Acad Sci U S A. 1982 Jan;79(2):267–271. [PMC free article] [PubMed]
  • Guiard B. Structure, expression and regulation of a nuclear gene encoding a mitochondrial protein: the yeast L(+)-lactate cytochrome c oxidoreductase (cytochrome b2). EMBO J. 1985 Dec 1;4(12):3265–3272. [PMC free article] [PubMed]
  • Harnisch U, Weiss H, Sebald W. The primary structure of the iron-sulfur subunit of ubiquinol-cytochrome c reductase from Neurospora, determined by cDNA and gene sequencing. Eur J Biochem. 1985 May 15;149(1):95–99. [PubMed]
  • Hartl FU, Schmidt B, Wachter E, Weiss H, Neupert W. Transport into mitochondria and intramitochondrial sorting of the Fe/S protein of ubiquinol-cytochrome c reductase. Cell. 1986 Dec 26;47(6):939–951. [PubMed]
  • Hase T, Müller U, Riezman H, Schatz G. A 70-kd protein of the yeast mitochondrial outer membrane is targeted and anchored via its extreme amino terminus. EMBO J. 1984 Dec 20;3(13):3157–3164. [PMC free article] [PubMed]
  • Hennig B, Neupert W. Assembly of cytochrome c. Apocytochrome c is bound to specific sites on mitochondria before its conversion to holocytochrome c. Eur J Biochem. 1981 Dec;121(1):203–212. [PubMed]
  • Hennig B, Koehler H, Neupert W. Receptor sites involved in posttranslational transport of apocytochrome c into mitochondria: specificity, affinity, and number of sites. Proc Natl Acad Sci U S A. 1983 Aug;80(16):4963–4967. [PMC free article] [PubMed]
  • Hurt EC, Müller U, Schatz G. The first twelve amino acids of a yeast mitochondrial outer membrane protein can direct a nuclear-coded cytochrome oxidase subunit to the mitochondrial inner membrane. EMBO J. 1985 Dec 16;4(13A):3509–3518. [PMC free article] [PubMed]
  • Kleene R, Pfanner N, Pfaller R, Link TA, Sebald W, Neupert W, Tropschug M. Mitochondrial porin of Neurospora crassa: cDNA cloning, in vitro expression and import into mitochondria. EMBO J. 1987 Sep;6(9):2627–2633. [PMC free article] [PubMed]
  • Pfaller R, Freitag H, Harmey MA, Benz R, Neupert W. A water-soluble form of porin from the mitochondrial outer membrane of Neurospora crassa. Properties and relationship to the biosynthetic precursor form. J Biol Chem. 1985 Jul 5;260(13):8188–8193. [PubMed]
  • Pfaller R, Neupert W. High-affinity binding sites involved in the import of porin into mitochondria. EMBO J. 1987 Sep;6(9):2635–2642. [PMC free article] [PubMed]
  • Pfanner N, Neupert W. Transport of proteins into mitochondria: a potassium diffusion potential is able to drive the import of ADP/ATP carrier. EMBO J. 1985 Nov;4(11):2819–2825. [PMC free article] [PubMed]
  • Pfanner N, Neupert W. Transport of F1-ATPase subunit beta into mitochondria depends on both a membrane potential and nucleoside triphosphates. FEBS Lett. 1986 Dec 15;209(2):152–156. [PubMed]
  • Pfanner N, Neupert W. Distinct steps in the import of ADP/ATP carrier into mitochondria. J Biol Chem. 1987 Jun 5;262(16):7528–7536. [PubMed]
  • Pfanner N, Tropschug M, Neupert W. Mitochondrial protein import: nucleoside triphosphates are involved in conferring import-competence to precursors. Cell. 1987 Jun 19;49(6):815–823. [PubMed]
  • Riezman H, Hay R, Witte C, Nelson N, Schatz G. Yeast mitochondrial outer membrane specifically binds cytoplasmically-synthesized precursors of mitochondrial proteins. EMBO J. 1983;2(7):1113–1118. [PMC free article] [PubMed]
  • Schleyer M, Neupert W. Transport of proteins into mitochondria: translocational intermediates spanning contact sites between outer and inner membranes. Cell. 1985 Nov;43(1):339–350. [PubMed]
  • Schmidt B, Hennig B, Zimmermann R, Neupert W. Biosynthetic pathway of mitochondrial ATPase subunit 9 in Neurospora crassa. J Cell Biol. 1983 Jan;96(1):248–255. [PMC free article] [PubMed]
  • Schmidt B, Wachter E, Sebald W, Neupert W. Processing peptidase of Neurospora mitochondria. Two-step cleavage of imported ATPase subunit 9. Eur J Biochem. 1984 Nov 2;144(3):581–588. [PubMed]
  • Stuart RA, Neupert W, Tropschug M. Deficiency in mRNA splicing in a cytochrome c mutant of neurospora crassa: importance of carboxy terminus for import of apocytochrome c into mitochondria. EMBO J. 1987 Jul;6(7):2131–2137. [PMC free article] [PubMed]
  • Takeda M, Vassarotti A, Douglas MG. Nuclear genes coding the yeast mitochondrial adenosine triphosphatase complex. Primary sequence analysis of ATP2 encoding the F1-ATPase beta-subunit precursor. J Biol Chem. 1985 Dec 15;260(29):15458–15465. [PubMed]
  • Viebrock A, Perz A, Sebald W. The imported preprotein of the proteolipid subunit of the mitochondrial ATP synthase from Neurospora crassa. Molecular cloning and sequencing of the mRNA. EMBO J. 1982;1(5):565–571. [PMC free article] [PubMed]
  • Zimmermann R, Neupert W. Transport of proteins into mitochondria. Posttranslational transfer of ADP/ATP carrier into mitochondria in vitro. Eur J Biochem. 1980 Aug;109(1):217–229. [PubMed]
  • Zwizinski C, Schleyer M, Neupert W. Transfer of proteins into mitochondria. Precursor to the ADP/ATP carrier binds to receptor sites on isolated mitochondria. J Biol Chem. 1983 Apr 10;258(7):4071–4074. [PubMed]
  • Zwizinski C, Schleyer M, Neupert W. Proteinaceous receptors for the import of mitochondrial precursor proteins. J Biol Chem. 1984 Jun 25;259(12):7850–7856. [PubMed]

Articles from The EMBO Journal are provided here courtesy of The European Molecular Biology Organization


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Compound
    PubChem Compound links
  • MedGen
    Related information in MedGen
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...