• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of embojLink to Publisher's site
EMBO J. Aug 1990; 9(8): 2579–2585.
PMCID: PMC552290

A new homeotic mutation in the Drosophila bithorax complex removes a boundary separating two domains of regulation.

Abstract

The bithorax complex specifies the identity of parasegments 5-14 of Drosophila. Although nine parasegment-specific functions, abx/bx, bxd/pbx and iab-2 to iab-8,9 have been identified, the whole bithorax complex appears to encode only three classes of proteins, Ubx, abd-A and Abd-B. Many observations suggest that the parasegment-specific functions act as positive cis-regulatory elements of Ubx, abd-A and Abd-B. We report the molecular genetics of a new gain-of-function mutation, Fab-7, which transforms parasegment 11 into parasegment 12. Induction of Abd-B mutations in cis (one of which removes the Abd-B homeobox) causes reversion of the dominant phenotype, demonstrating that Fab-7 misregulates Abd-B. A 4 kb deletion, 30 kb downstream from the Abd-B transcription unit, is solely responsible for the Fab-7 phenotype. We consider that the parasegment-specific functions lie in DNA domains that are sequentially and independently 'opened' along the chromosome. Once a domain is opened, the cis-regulatory sequences within it can carry out their function. We propose that the Fab-7 deletion removes a boundary separating the iab-6 and iab-7 cis-regulatory regions (the functions specific for parasegments 11 and 12) allowing the open configuration of iab-6 to invade iab-7 in parasegment 11. This is strongly supported by our finding that Fab-7 can be caused to revert by lesions not only in iab-7 but also in iab-6.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.9M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Pirrotta V, Steller H, Bozzetti MP. Multiple upstream regulatory elements control the expression of the Drosophila white gene. EMBO J. 1985 Dec 16;4(13A):3501–3508. [PMC free article] [PubMed]
  • Rowe A, Akam M. The structure and expression of a hybrid homeotic gene. EMBO J. 1988 Apr;7(4):1107–1114. [PMC free article] [PubMed]
  • Sánchez-Herrero E, Crosby MA. The Abdominal-B gene of Drosophila melanogaster: overlapping transcripts exhibit two different spatial distributions. EMBO J. 1988 Jul;7(7):2163–2173. [PMC free article] [PubMed]
  • Sánchez-Herrero E, Akam M. Spatially ordered transcription of regulatory DNA in the bithorax complex of Drosophila. Development. 1989 Oct;107(2):321–329. [PubMed]
  • Sánchez-Herrero E, Vernós I, Marco R, Morata G. Genetic organization of Drosophila bithorax complex. Nature. 1985 Jan 10;313(5998):108–113. [PubMed]
  • Struhl G, Akam M. Altered distributions of Ultrabithorax transcripts in extra sex combs mutant embryos of Drosophila. EMBO J. 1985 Dec 1;4(12):3259–3264. [PMC free article] [PubMed]
  • Tiong S, Bone LM, Whittle JR. Recessive lethal mutations within the bithorax-complex in Drosophila. Mol Gen Genet. 1985;200(2):335–342. [PubMed]
  • Zavortink M, Sakonju S. The morphogenetic and regulatory functions of the Drosophila Abdominal-B gene are encoded in overlapping RNAs transcribed from separate promoters. Genes Dev. 1989 Dec;3(12A):1969–1981. [PubMed]
  • Akam M. The molecular basis for metameric pattern in the Drosophila embryo. Development. 1987 Sep;101(1):1–22. [PubMed]
  • Beachy PA, Helfand SL, Hogness DS. Segmental distribution of bithorax complex proteins during Drosophila development. Nature. 1985 Feb 14;313(6003):545–551. [PubMed]
  • Bender W, Spierer P, Hogness DS. Chromosomal walking and jumping to isolate DNA from the Ace and rosy loci and the bithorax complex in Drosophila melanogaster. J Mol Biol. 1983 Jul 25;168(1):17–33. [PubMed]
  • Bender W, Akam M, Karch F, Beachy PA, Peifer M, Spierer P, Lewis EB, Hogness DS. Molecular Genetics of the Bithorax Complex in Drosophila melanogaster. Science. 1983 Jul 1;221(4605):23–29. [PubMed]
  • Casanova J, Sánchez-Herrero E, Morata G. Identification and characterization of a parasegment specific regulatory element of the abdominal-B gene of Drosophila. Cell. 1986 Nov 21;47(4):627–636. [PubMed]
  • Casanova J, Sánchez-Herrero E, Busturia A, Morata G. Double and triple mutant combinations of bithorax complex of Drosophila. EMBO J. 1987 Oct;6(10):3103–3109. [PMC free article] [PubMed]
  • Casanova J, Sánchez-Herrero E, Morata G. Developmental analysis of a hybrid gene composed of parts of the Ubx and abd-A genes of Drosophila. EMBO J. 1988 Apr;7(4):1097–1105. [PMC free article] [PubMed]
  • Celniker SE, Lewis EB. Transabdominal, a dominant mutant of the Bithorax Complex, produces a sexually dimorphic segmental transformation in Drosophila. Genes Dev. 1987 Apr;1(2):111–123. [PubMed]
  • DeLorenzi M, Ali N, Saari G, Henry C, Wilcox M, Bienz M. Evidence that the Abdominal-B r element function is conferred by a trans-regulatory homeoprotein. EMBO J. 1988 Oct;7(10):3223–3231. [PMC free article] [PubMed]
  • Duboule D, Dollé P. The structural and functional organization of the murine HOX gene family resembles that of Drosophila homeotic genes. EMBO J. 1989 May;8(5):1497–1505. [PMC free article] [PubMed]
  • Duncan IM. Polycomblike: a gene that appears to be required for the normal expression of the bithorax and antennapedia gene complexes of Drosophila melanogaster. Genetics. 1982 Sep;102(1):49–70. [PMC free article] [PubMed]
  • Duncan I. The bithorax complex. Annu Rev Genet. 1987;21:285–319. [PubMed]
  • Feinberg AP, Vogelstein B. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984 Feb;137(1):266–267. [PubMed]
  • Frischauf AM, Lehrach H, Poustka A, Murray N. Lambda replacement vectors carrying polylinker sequences. J Mol Biol. 1983 Nov 15;170(4):827–842. [PubMed]
  • Graham A, Papalopulu N, Krumlauf R. The murine and Drosophila homeobox gene complexes have common features of organization and expression. Cell. 1989 May 5;57(3):367–378. [PubMed]
  • Ingham PW. The molecular genetics of embryonic pattern formation in Drosophila. Nature. 1988 Sep 1;335(6185):25–34. [PubMed]
  • Karch F, Weiffenbach B, Peifer M, Bender W, Duncan I, Celniker S, Crosby M, Lewis EB. The abdominal region of the bithorax complex. Cell. 1985 Nov;43(1):81–96. [PubMed]
  • Kollias G, Hurst J, deBoer E, Grosveld F. The human beta-globin gene contains a downstream developmental specific enhancer. Nucleic Acids Res. 1987 Jul 24;15(14):5739–5747. [PMC free article] [PubMed]
  • Kornfeld K, Saint RB, Beachy PA, Harte PJ, Peattie DA, Hogness DS. Structure and expression of a family of Ultrabithorax mRNAs generated by alternative splicing and polyadenylation in Drosophila. Genes Dev. 1989 Feb;3(2):243–258. [PubMed]
  • Kuziora MA, McGinnis W. Different transcripts of the Drosophila Abd-B gene correlate with distinct genetic sub-functions. EMBO J. 1988 Oct;7(10):3233–3244. [PMC free article] [PubMed]
  • Lewis EB. A gene complex controlling segmentation in Drosophila. Nature. 1978 Dec 7;276(5688):565–570. [PubMed]
  • Martinez-Arias A, Lawrence PA. Parasegments and compartments in the Drosophila embryo. Nature. 1985 Feb 21;313(6004):639–642. [PubMed]
  • O'Connor MB, Binari R, Perkins LA, Bender W. Alternative RNA products from the Ultrabithorax domain of the bithorax complex. EMBO J. 1988 Feb;7(2):435–445. [PMC free article] [PubMed]
  • O'Kane CJ, Gehring WJ. Detection in situ of genomic regulatory elements in Drosophila. Proc Natl Acad Sci U S A. 1987 Dec;84(24):9123–9127. [PMC free article] [PubMed]

Articles from The EMBO Journal are provided here courtesy of The European Molecular Biology Organization

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...