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Journal List > Infect Immun > v.29(2); Aug 1980

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Infect Immun. 1980 August; 29(2): 564–574.
PMCID: PMC551156
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Antigenic Studies of Oral and Nonoral Black-Pigmented Bacteroides Strains
Michael J. Reed,† Jørgen Slots, Christian Mouton, and Robert J. Genco
Department of Oral Biology and Periodontal Disease, Clinical Research Center, State University of New York at Buffalo, Buffalo, New York 14226
†Present address: School of Dentistry, University of Mississippi Medical Center, Jackson, MS 39216.
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Abstract
Antigens of several oral and nonoral strains of Bacteroides asaccharolyticus (proposed classification of oral B. asaccharolyticus, Bacteroides gingivalis), Bacteroides melaninogenicus subsp. intermedius, B. melaninogenicus subsp. melaninogenicus, and B. melaninogenicus subsp. levii were identified in soluble preparations obtained by sonication, autoclaving, and NaOH treatment of whole bacterial cells. The sonicate preparations contained the most complete representation of soluble antigens using antisera to the whole organism in gel precipitation tests. Among strains of B. melaninogenicus subsp. intermedius many common antigens were detected, and no consistent antigenic differences were seen between strains from oral and nonoral sites. None of the antigens of B. melaninogenicus subsp. intermedius reacted with sera raised to several strains of oral or nonoral B. asaccharolyticus, nor did antigens prepared from the latter strains react with antisera to B. melaninogenicus subsp. intermedius. At least one common antigen was shared by strains of B. melaninogenicus subsp. intermedius and strains of B. melaninogenicus subsp. melaninogenicus; however, subspecies-specific antigens were also found. Antigens from and antisera to oral and nonoral strains of B. asaccharolyticus did not react with sera to and antigens from B. melaninogenicus subsp. melaninogenicus. Strains of B. asaccharolyticus isolated from the oral cavity were antigenically distinct from strains of B. asaccharolyticus obtained from nonoral sites and lesions. This lack of cross-reactivity between the oral and nonoral strains of B. asaccharolyticus together with recent findings of marked genetic differences between oral and nonoral strains of B. asaccharolyticus suggest that these groups of organisms may represent different species.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
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