• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Oct 1990; 87(20): 8105–8109.

Cleavage of tRNA within the mature tRNA sequence by the catalytic RNA of RNase P: implication for the formation of the primer tRNA fragment for reverse transcription in copia retrovirus-like particles.


The retrovirus-like particles of Drosophila are intermediates of retrotransposition of the transposable element copia. In these particles, a 39-nucleotide-long fragment from the 5' region of Drosophila initiator methionine tRNA (tRNA(iMet) is used as the primer for copia minus-strand reverse transcription. To function as primer for this reverse transcription, the Drosophila tRNA(iMet) must be cleaved in vivo at the site between nucleotides 39 and 40. When a synthetic Drosophila tRNA(iMet) precursor was incubated with M1RNA, the catalytic RNA of Escherichia coli RNase P, other cleavages within the mature tRNA sequence were detected in addition to the efficient removal of the 5' leader sequence of this tRNA precursor. One of these cleavage sites is between nucleotides 39 and 40 of Drosophila tRNA(iMet). Based on this result, we propose a model for formation of the primer tRNA fragment for reverse transcription in copia retrovirus-like particles.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Shiba T, Saigo K. Retrovirus-like particles containing RNA homologous to the transposable element copia in Drosophila melanogaster. Nature. 1983 Mar 10;302(5904):119–124. [PubMed]
  • Kikuchi Y, Ando Y, Shiba T. Unusual priming mechanism of RNA-directed DNA synthesis in copia retrovirus-like particles of Drosophila. Nature. 323(6091):824–826. [PubMed]
  • Sharp S, DeFranco D, Silberklang M, Hosbach HA, Schmidt T, Kubli E, Gergen JP, Wensink PC, Söll D. The initiator tRNA genes of Drosophila melanogaster: evidence for a tRNA pseudogene. Nucleic Acids Res. 1981 Nov 25;9(22):5867–5882. [PMC free article] [PubMed]
  • Guerrier-Takada C, Gardiner K, Marsh T, Pace N, Altman S. The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme. Cell. 1983 Dec;35(3 Pt 2):849–857. [PubMed]
  • McClain WH, Guerrier-Takada C, Altman S. Model substrates for an RNA enzyme. Science. 1987 Oct 23;238(4826):527–530. [PubMed]
  • Kikuchi Y, Hishinuma F, Sakaguchi K. Addition of mononucleotides to oligoribonucleotide acceptors with T4 RNA ligase. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1270–1273. [PMC free article] [PubMed]
  • Guerrier-Takada C, van Belkum A, Pleij CW, Altman S. Novel reactions of RNAase P with a tRNA-like structure in turnip yellow mosaic virus RNA. Cell. 1988 Apr 22;53(2):267–272. [PubMed]
  • Green CJ, Vold BS, Morch MD, Joshi RL, Haenni AL. Ionic conditions for the cleavage of the tRNA-like structure of turnip yellow mosaic virus by the catalytic RNA of RNase P. J Biol Chem. 1988 Aug 25;263(24):11617–11620. [PubMed]
  • Barat C, Lullien V, Schatz O, Keith G, Nugeyre MT, Grüninger-Leitch F, Barré-Sinoussi F, LeGrice SF, Darlix JL. HIV-1 reverse transcriptase specifically interacts with the anticodon domain of its cognate primer tRNA. EMBO J. 1989 Nov;8(11):3279–3285. [PMC free article] [PubMed]
  • Masukata H, Tomizawa J. Control of primer formation for ColE1 plasmid replication: conformational change of the primer transcript. Cell. 1986 Jan 17;44(1):125–136. [PubMed]
  • Yanofsky C. Attenuation in the control of expression of bacterial operons. Nature. 1981 Feb 26;289(5800):751–758. [PubMed]
  • Lawrence N, Wesolowski D, Gold H, Bartkiewicz M, Guerrier-Takada C, McClain WH, Altman S. Characteristics of ribonuclease P from various organisms. Cold Spring Harb Symp Quant Biol. 1987;52:233–238. [PubMed]
  • Bartkiewicz M, Gold H, Altman S. Identification and characterization of an RNA molecule that copurifies with RNase P activity from HeLa cells. Genes Dev. 1989 Apr;3(4):488–499. [PubMed]
  • Lee JY, Engelke DR. Partial characterization of an RNA component that copurifies with Saccharomyces cerevisiae RNase P. Mol Cell Biol. 1989 Jun;9(6):2536–2543. [PMC free article] [PubMed]
  • Carrara G, Calandra P, Fruscoloni P, Doria M, Tocchini-Valentini GP. Site selection by Xenopus laevis RNAase P. Cell. 1989 Jul 14;58(1):37–45. [PubMed]
  • Flavell AJ, Ish-Horowicz D. The origin of extrachromosomal circular copia elements. Cell. 1983 Sep;34(2):415–419. [PubMed]
  • Flavell AJ, Brierley C. The termini of extrachromosomal linear copia elements. Nucleic Acids Res. 1986 May 12;14(9):3659–3669. [PMC free article] [PubMed]
  • Taylor J, Sharmeen L. Retrovirus genome replication: priming specificities of plus-strand DNA synthesis. J Cell Sci Suppl. 1987;7:189–195. [PubMed]
  • Yudelevich A. Specific cleavage of an Escherichia coli leucine transfer RNA following bacteriophage T4 infection. J Mol Biol. 1971 Aug 28;60(1):21–29. [PubMed]
  • David M, Borasio GD, Kaufmann G. Bacteriophage T4-induced anticodon-loop nuclease detected in a host strain restrictive to RNA ligase mutants. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7097–7101. [PMC free article] [PubMed]
  • Weiner AM, Deininger PL, Efstratiadis A. Nonviral retroposons: genes, pseudogenes, and transposable elements generated by the reverse flow of genetic information. Annu Rev Biochem. 1986;55:631–661. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...