• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Oct 1990; 87(19): 7633–7637.

Wound-induced accumulation of mRNA containing a hevein sequence in laticifers of rubber tree (Hevea brasiliensis).


Hevein is a chitin-binding protein that is present in laticifers of the rubber tree (Hevea brasiliensis). A cDNA clone (HEV1) encoding hevein was isolated by using the polymerase chain reaction with mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea latex cDNA library as a template. HEV1 is 1018 base pairs long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187-amino acid polypeptide. This polypeptide has two striking features. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino termini of wound-inducible proteins in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74-79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems, and latex but not in roots.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Wright CS, Gavilanes F, Peterson DL. Primary structure of wheat germ agglutinin isolectin 2. Peptide order deduced from X-ray structure. Biochemistry. 1984 Jan 17;23(2):280–287. [PubMed]
  • Raikhel NV, Wilkins TA. Isolation and characterization of a cDNA clone encoding wheat germ agglutinin. Proc Natl Acad Sci U S A. 1987 Oct;84(19):6745–6749. [PMC free article] [PubMed]
  • Lerner DR, Raikhel NV. Cloning and characterization of root-specific barley lectin. Plant Physiol. 1989 Sep;91(1):124–129. [PMC free article] [PubMed]
  • Wilkins TA, Raikhel NV. Expression of rice lectin is governed by two temporally and spatially regulated mRNAs in developing embryos. Plant Cell. 1989 May;1(5):541–549. [PMC free article] [PubMed]
  • Broglie KE, Gaynor JJ, Broglie RM. Ethylene-regulated gene expression: molecular cloning of the genes encoding an endochitinase from Phaseolus vulgaris. Proc Natl Acad Sci U S A. 1986 Sep;83(18):6820–6824. [PMC free article] [PubMed]
  • Shinshi H, Mohnen D, Meins F. Regulation of a plant pathogenesis-related enzyme: Inhibition of chitinase and chitinase mRNA accumulation in cultured tobacco tissues by auxin and cytokinin. Proc Natl Acad Sci U S A. 1987 Jan;84(1):89–93. [PMC free article] [PubMed]
  • Parsons TJ, Bradshaw HD, Jr, Gordon MP. Systemic accumulation of specific mRNAs in response to wounding in poplar trees. Proc Natl Acad Sci U S A. 1989 Oct;86(20):7895–7899. [PMC free article] [PubMed]
  • Stanford A, Bevan M, Northcote D. Differential expression within a family of novel wound-induced genes in potato. Mol Gen Genet. 1989 Jan;215(2):200–208. [PubMed]
  • Kush A, Goyvaerts E, Chye ML, Chua NH. Laticifer-specific gene expression in Hevea brasiliensis (rubber tree). Proc Natl Acad Sci U S A. 1990 Mar;87(5):1787–1790. [PMC free article] [PubMed]
  • Silflow CD, Hammett JR, Key JL. Sequence complexity of polyadenylated ribonucleic acid from soybean suspension culture cells. Biochemistry. 1979 Jun 26;18(13):2725–2731. [PubMed]
  • Gubler U, Hoffman BJ. A simple and very efficient method for generating cDNA libraries. Gene. 1983 Nov;25(2-3):263–269. [PubMed]
  • Vieira J, Messing J. Production of single-stranded plasmid DNA. Methods Enzymol. 1987;153:3–11. [PubMed]
  • Feinberg AP, Vogelstein B. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984 Feb;137(1):266–267. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Mizusawa S, Nishimura S, Seela F. Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP. Nucleic Acids Res. 1986 Feb 11;14(3):1319–1324. [PMC free article] [PubMed]
  • Dale RM, Arrow A. A rapid single-stranded cloning, sequencing, insertion, and deletion strategy. Methods Enzymol. 1987;155:204–214. [PubMed]
  • Kirk MM, Kirk DL. Translational regulation of protein synthesis, in response to light, at a critical stage of Volvox development. Cell. 1985 Jun;41(2):419–428. [PubMed]
  • von Heijne G. Signal sequences. The limits of variation. J Mol Biol. 1985 Jul 5;184(1):99–105. [PubMed]
  • Boutry M, Chua NH. A nuclear gene encoding the beta subunit of the mitochondrial ATP synthase in Nicotiana plumbaginifolia. EMBO J. 1985 Sep;4(9):2159–2165. [PMC free article] [PubMed]
  • Broekaert WF, VAN Parijs J, Leyns F, Joos H, Peumans WJ. A chitin-binding lectin from stinging nettle rhizomes with antifungal properties. Science. 1989 Sep 8;245(4922):1100–1102. [PubMed]
  • Bohlmann H, Clausen S, Behnke S, Giese H, Hiller C, Reimann-Philipp U, Schrader G, Barkholt V, Apel K. Leaf-specific thionins of barley-a novel class of cell wall proteins toxic to plant-pathogenic fungi and possibly involved in the defence mechanism of plants. EMBO J. 1988 Jun;7(6):1559–1565. [PMC free article] [PubMed]
  • Hedrick SA, Bell JN, Boller T, Lamb CJ. Chitinase cDNA cloning and mRNA induction by fungal elicitor, wounding, and infection. Plant Physiol. 1988 Jan;86(1):182–186. [PMC free article] [PubMed]
  • Ecker JR, Davis RW. Plant defense genes are regulated by ethylene. Proc Natl Acad Sci U S A. 1987 Aug;84(15):5202–5206. [PMC free article] [PubMed]
  • Pēna-Cortés H, Sánchez-Serrano JJ, Mertens R, Willmitzer L, Prat S. Abscisic acid is involved in the wound-induced expression of the proteinase inhibitor II gene in potato and tomato. Proc Natl Acad Sci U S A. 1989 Dec;86(24):9851–9855. [PMC free article] [PubMed]
  • Henstrand JM, Handa AK. Effect of Ethylene Action Inhibitors upon Wound-Induced Gene Expression in Tomato Pericarp. Plant Physiol. 1989 Sep;91(1):157–162. [PMC free article] [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...