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Journal List > Appl Microbiol > v.15(3); May 1967

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Appl Microbiol. 1967 May; 15(3): 468–470.
PMCID: PMC546944
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Independent Measurement of Ampicillin and Cloxacillin in Mixtures
Leon D. Sabath, Valerie J. Estey, and Maxwell Finland
Thorndike Memorial Laboratory, Second and Fourth (Harvard) Medical Services, Boston City Hospital, Boston, Massachusetts 02118
Department of Medicine, Harvard Medical School, Boston, Massachusetts 02118
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Abstract
A simplified method of assaying mixtures of ampicillin and cloxacillin is presented. The method consists of cylinder-plate assays of appropriately diluted samples in three assay systems in which: (i) cloxacillin is measured, utilizing agar seeded with a penicillinase-producing Staphylococcus aureus; (ii) ampicillin is measured after the greatest dilution practicable, by use of agar seeded with Sarcina lutea; and (iii) the proportion of activity found in the second assay that remains after treatment with staphylococcal penicillinase is determined on agar seeded with S. lutea. The error was 4% or less when each system was run in quadruplicate, except when there was 10 or more times as much cloxacillin as ampicillin, in which case the greatest error was 12%. Mixtures of methicillin with benzylpenicillin or with ampicillin can also be quantitated by this method.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
  • COLE M, ROLINSON GN. 6-Aminopenicillanic acid. II. Formation of 6-aminopenicillanic acid by Emericellopsis minima (Stolk) and related fungi. Proc R Soc Lond B Biol Sci. 1961 Aug 15;154:490–497. [PubMed]
  • Heatley NG. A method for the assay of penicillin. Biochem J. 1944;38(1):61–65. [PubMed]
  • Kirby WM. PROPERTIES OF A PENICILLIN INACTIVATOR EXTRACTED FROM PENICILLIN-RESISTANT STAPHYLOCOCCI. J Clin Invest. 1945 Mar;24(2):170–174. [PubMed]
  • SABATH LD, ABRAHAM EP. SYNERGISTIC ACTION OF PENICILLINS AND CEPHALOSPORINS AGAINST PSEUDOMONAS PYOCYANEA. Nature. 1964 Dec 12;204:1066–1069. [PubMed]
  • Sabath LD, McCall CE, Steigbigel NH, Finland M. Synergistic penicillin combinations for treatment of human urinary-tract infections. Antimicrob Agents Chemother (Bethesda). 1966;6:149–155. [PubMed]
  • SABATH LD, FINLAND M. Sterile penicillinase from phagelysed staphylococci. Nature. 1962 Jan 20;193:272–273. [PubMed]
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