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Figure 1

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Substrates with 3′-PG termini. (A) Oligomeric substrate. The 3′-PG terminus can be converted to a 3′-phosphate by TDP1 and thence to a 3′-OH by PNKP. (B) The 5.5 kb 3′-PG plasmid substrate is internally labeled, and 3′-terminal processing can be monitored by cleaving the substrate with AvaI and analyzing the labeled fragment by denaturing gel electrophoresis.

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