• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of plntphysLink to Publisher's site
Plant Physiol. Mar 1974; 53(3): 426–433.
PMCID: PMC543240

Structural and Functional Responses of Wheat Mitochondrial Membranes to Growth at Low Temperatures 1

Abstract

The responses in membrane lipid composition, structure, and function of four cultivars of wheat (Triticum aestivum L.) to growth at low temperature have been investigated. Marked growth temperature-dependent alterations in the fatty acid composition and unsaturation of the mitochondrial phospholipids correlate with changes in respiratory activity in all the varieties. Parameters such as the respiratory control ratio and the phosphorylative efficiency decrease in cold-adapted seedlings. Three temperature-dependent structural transitions were identified in the mitochondria by the spin-labeling method. The structural transitions occur at lower temperatures in the cold-grown material. The shift in one transition appears to be quantitatively greater in the winter hardy varieties. Cold-induced changes in all of the other measured parameters were indistinguishable in hardy and nonhardy varieties. The results indicate major involvement of the phospholipid matrix in cold acclimation. A link between cold acclimation and winter survival may exist involving the structural and functional modifications in membrane structure which occur during acclimation.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Allen RJ. The estimation of phosphorus. Biochem J. 1940 Jun;34(6):858–865. [PMC free article] [PubMed]
  • Barratt MD, Green DK, Chapman D. Spin-labelled lipid-protein complexes. Biochim Biophys Acta. 1968 Jan 10;152(1):20–27. [PubMed]
  • de la Roche IA, Andrews CJ. Changes in Phospholipid Composition of a Winter Wheat Cultivar during Germination at 2 C and 24 C. Plant Physiol. 1973 Mar;51(3):468–473. [PMC free article] [PubMed]
  • Eletr S, Keith AD. Spin-label studies of dynamics of lipid alkyl chains in biological membranes: role of unsaturated sites. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1353–1357. [PMC free article] [PubMed]
  • Heber U. Freezing injury in relation to loss of enzyme activities and protection against freezing. Cryobiology. 1968 Nov-Dec;5(3):188–201. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Lyons JM, Asmundson CM. Solidification of unsaturated/saturated fatty acid mixtures and its relationship to chilling sensitivity in plants. J Am Oil Chem Soc. 1965 Dec;42(12):1056–1058. [PubMed]
  • Lyons JM, Raison JK. Oxidative activity of mitochondria isolated from plant tissues sensitive and resistant to chilling injury. Plant Physiol. 1970 Apr;45(4):386–389. [PMC free article] [PubMed]
  • Raison JK, Lyons JM, Mehlhorn RJ, Keith AD. Temperature-induced phase changes in mitochondrial membranes detected by spin labeling. J Biol Chem. 1971 Jun 25;246(12):4036–4040. [PubMed]
  • Sarkissian IV, Srivastava HK. On methods of isolation of active, tightly coupled mitochondria of wheat seedlings. Plant Physiol. 1968 Sep;43(9):1406–1410. [PMC free article] [PubMed]
  • Siminovitch D, Rheaume B, Pomeroy K, Lepage M. Phospholipid, protein, and nucleic acid increases in protoplasm and membrane structures associated with development of extreme freezing resistance in black locust tree cells. Cryobiology. 1968 Nov-Dec;5(3):202–225. [PubMed]
  • Tinberg HM, Packer L, Keith AD. Role of lipids in mitochondrial energy coupling: evidence from spin labeling and freeze-fracture electron microscopy. Biochim Biophys Acta. 1972 Nov 17;283(2):193–205. [PubMed]
  • Yamaki S, Uritani I. Mechanism of Chilling Injury in Sweet Potato: X. Change in Lipid-Protein Interaction in Mitochondria from Cold-stored Tissue. Plant Physiol. 1973 May;51(5):883–888. [PMC free article] [PubMed]

Articles from Plant Physiology are provided here courtesy of American Society of Plant Biologists

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...