• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of plntphysLink to Publisher's site
Plant Physiol. Sep 1976; 58(3): 315–319.
PMCID: PMC542238

Cytochemical Localization of Glycolate Dehydrogenase in Mitochondria of Chlamydomonas1

Abstract

Mildly disrupted cells of Chlamydomonas reinhardi Dangeard were incubated in a reaction medium containing glycolate, ferricyanide, and cupric ions, and then processed for electron microscopy. As a result of the cytochemical treatment, an electron opaque product was deposited specifically in the outer compartment of mitochondria; other cellular components, including microbodies, did not accumulate stain. Incubation with d-lactate yielded similar results, while treatment with l-lactate produced only a weak reaction. Oxamate, which inhibits glycolate dehydrogenase activity in cell-free extracts, also inhibited the cytochemical reaction. These findings demonstrate in situ that glycolate dehydrogenase is localized in mitochondria, and thus corroborate similar conclusions reached on the basis of enzymic studies of isolated algal organelles.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (2.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Burke JJ, Trelease RN. Cytochemical demonstration of malate synthase and glycolate oxidase in microbodies of cucumber cotyledons. Plant Physiol. 1975 Nov;56(5):710–717. [PMC free article] [PubMed]
  • Codd GA, Lord JM, Merrett MJ. The glycollate oxidising enzyme of algae. FEBS Lett. 1969 Dec 30;5(5):341–342. [PubMed]
  • Collins N, Brown RH, Merrett MJ. Oxidative phosphorylation during glycollate metabolism in mitochondria from phototrophic Euglena gracilis. Biochem J. 1975 Sep;150(3):373–377. [PMC free article] [PubMed]
  • Collins N, Merrett MJ. The localization of glycollate-pathway enzymes in Euglena. Biochem J. 1975 May;148(2):321–328. [PMC free article] [PubMed]
  • Curtis VA, Brand JJ, Togasaki RK. Partial reactions of photosynthesis in briefly sonicated chlamydomonas: I. Cell breakage and electron transport activities. Plant Physiol. 1975 Feb;55(2):183–186. [PMC free article] [PubMed]
  • Frederick SE, Gruber PJ, Tolbert NE. The occurrence of glycolate dehydrogenase and glycolate oxidase in green plants: an evolutionary survey. Plant Physiol. 1973 Oct;52(4):318–323. [PMC free article] [PubMed]
  • Gruber PJ, Frederick SE, Tolbert NE. Enzymes related to lactate metabolism in green algae and lower land plants. Plant Physiol. 1974 Feb;53(2):167–170. [PMC free article] [PubMed]
  • Hand AR. Ultrastructural localization of L-alpha-hydroxy acid oxidase in rat liver perioxisomes. Histochemistry. 1975;41(3):195–206. [PubMed]
  • Huang AH, Beevers H. Localization of enzymes within microbodies. J Cell Biol. 1973 Aug;58(2):379–389. [PMC free article] [PubMed]
  • Nelson EB, Tolbert NE. Glycolate dehydrogenase in green algae. Arch Biochem Biophys. 1970 Nov;141(1):102–110. [PubMed]
  • Paul JS, Sullivan CS, Bolxani BE. Photorespiration in diatoms. Mitochondrial glycolate dehydrogenase in clyindrotheca fusiformis and Nitzschia alba. Arch Biochem Biophys. 1975 Jul;169(1):152–159. [PubMed]
  • Shnitka TK, Seligman AM. Ultrastructural localization of enzymes. Annu Rev Biochem. 1971;40:375–396. [PubMed]
  • Spurr AR. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969 Jan;26(1):31–43. [PubMed]
  • Stabenau H. Localization of Enzymes of Glycolate Metabolism in the Alga Chlorogonium elongatum. Plant Physiol. 1974 Dec;54(6):921–924. [PMC free article] [PubMed]

Articles from Plant Physiology are provided here courtesy of American Society of Plant Biologists

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...